Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (4): 218-222.doi: 10.12016/j.issn.2096-1456.2016.04.004

• Basic Study • Previous Articles     Next Articles

A study on enamel demineralization produced by citric acid

Cheng-xia LIU1,Jian-zhen YANG1,Hui-li NING1,Tao GONG1,Su-lan ZHONG1,Xiao-qing SHEN2()   

  1. 1. Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China
  • Received:2015-11-16 Revised:2015-12-30 Online:2016-04-20 Published:2016-04-20
  • Contact: Xiao-qing SHEN E-mail:1061787412@qq.com

Abstract:

Objective To observe the effect of different etching time of citric acid on enamel blocks to acquire a reliable in vitro model for early enamel caries, and explore more effective experiment method for enamel demineralization.Methods Human premolars were selected and made into enamel blocks. The morphologies of enamel blocks surfaces etched by 10% citric acid for different time periods (90 s, 120 s, 150 s) and partially saturated acid buffer demineralization system for a week were observed by scanning electron microscope. And the surface micro hardness of these specimen before and after demineralization were tested by the micro hardness tester.Results After demineralization, enamel surface had an obvious acid etching appearance. In the 90 s etching time group, only a small part of enamel prism lattice was demineralized and dissolved, and the ability of demineralization was limited. In 120 s etching time group, the morphology of enamel blocks surface was similar to natural early enamel lesions, like the honeycomb. In the 150 s etching time group, the enamel surface had an acid corrosion damage morphology, and it was excessive etched. However,the demineralization effect of partially saturated acid buffer demineralization system was relatively low.Conclusion Citric acid has a strong etching effect, and its ability of demineralization is enhanced with the extension of time. The model of early enamel lesions can be obtained by 10% citric acid for 120 s.

Key words: Citric acid, Early enamel caries, Enamel, Scanning electron microscope, Demineralization

CLC Number: 

  • R780.1

"

"

组别 基线表面显微硬度 脱矿后表面显微硬度 脱矿率(%) t P
90 s组 331.4±10.4 232.0±5.7 29.9±2.6 28.636 <0.001
120 s组 328.3±8.8 170.5±4.2 48.0±1.5 60.732 <0.001
150 s组 327.3±10.0 130.5±4.0 60.1±1.1 73.593 <0.001
部分饱和酸缓冲脱矿系统组 332.6±5.6 259.5±3.7 22.0±0.9 59.270 <0.001
F 0.789 1 714 1 095
P 0.508 <0.001 <0.001
[1] 黄冠玮, 邹玲 . 人工龋模型的建立方法[J]. 国际口腔医学杂志, 2010,37(5):537-540.
doi: 10.3969/j.issn.1673-5749.2010.05.012
[2] 叶玲, 苏勤 . 体外人工龋模型的建立方法及检测新技术[J]. 广东牙病防治, 2002,10(4):310-312.
doi: 10.3969/j.issn.1006-5245.2002.04.043
[3] 元佩燕, 徐平平, 黄建生 , 等. 枸橼酸酸蚀牙本质的最佳浓度和时间[J]. 中国组织工程研究, 2012,16(16):2959-2963.
doi: 10.3969/j.issn.1673-8225.2012.16.026
[4] 吴瑞 . 人牙釉质在改性柠檬酸介质中的酸蚀行为研究[D]. 成都:西南交通大学, 2014.
[5] 朱文婷, 汪俊, 岑莲 . 柠檬酸酸蚀后再植牙根表面的脱矿研究[J]. 组织工程与重建外科杂志, 2014,10(5):259-262.
[6] 高峰, 李涢 . 柠檬酸对牙本质酸蚀作用的体外研究[J]. 现代口腔医学杂志, 2015,29(3):144-148.
[7] 楚金普, 郝玉庆, 李继遥 , 等. 中药五倍子化学成分对早期龋再矿化作用的扫描电镜观察[J]. 时珍国医国药, 2009,20(6):1306-1307.
doi: 10.3969/j.issn.1008-0805.2009.06.005
[8] 王海芹, 符大勇, 张秀荣 , 等. 年轻恒牙与成人恒牙釉质酸蚀时间的研究[J]. 广东牙病防治, 2014,22(5):234-237.
[9] Huang S, Gao S, Cheng L , et al. Combined effects of nano-hydroxyapatite and Galla chinensis on remineralisation of initial enamel lesion in vitro[J]. J Dent, 2010,38(10):811-819.
doi: 10.1016/j.jdent.2010.06.013 pmid: 20619311
[10] 马伟群, 黄建生, 徐平平 . 掺羟基磷灰石牙膏对早期龋的修复及吸附镉离子作用研究[J]. 日用化学工业, 2014,44(5):266-269.
doi: 10.13218/j.cnki.csdc.2014.05.059
[11] Möller H, Schröder U . Early natural subsurface caries. A SEM study of the enamel surface before and after remineralization[J]. Caries Res, 1986,20(2):97-102.
doi: 10.1159/000260927
[12] 张玲, 王绮, 张炜 . 儿童龋病预防方法的研究进展[J]. 临床合理用药杂志, 2014,7(17):174-175.
doi: 10.3969/j.issn.1674-3296.2014.17.145
[13] 齐小秋 . 第三次全国口腔健康流行病学调查报告[M]. 北京: 人民卫生出版社, 2008.
[14] Borsboom PC, vd Mei HC, Arends J . Enamel lesion formation with and without 0.12 ppm F in solution[J]. Caries Res, 1985,19(5):396-402.
doi: 10.1159/000260873 pmid: 3864541
[15] Fejerskov O, Kidd EAM . Dental caries: the disease and its clinical management[M]. 2nd Edition. Copenhagen: Blackwell Munksgaard, 2003: 80-83.
[16] Amaechi BT, Higham SM, Edgar WM . Factors influencing the development of dental erosion in vitro: enamel type, temperature and exposure time[J]. J Oral Rehabil, 1999,26(8):624-630.
doi: 10.1046/j.1365-2842.1999.00433.x pmid: 10447814
[17] Yoshida Y, Van Meerbeek B, Nakayama Y , et al. Adhesion to and de-calcification of hydroxyapatite by carboxylic acids[J]. J Dent Res, 2001,80(6):1565-1569.
[1] XIONG Kaixin,ZOU Ling. Research progress in laser treatment of dentin hypersensitivity [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 472-476.
[2] LU Lizhu, QIU Hongtian, CAI Qiuyun, ZHOU Wei. Progress in clinical treatment and etiology of gingival recession [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(5): 331-336.
[3] HU Beibei,BAI Hai,JIA Wanping,LIANG Yongqiang. Remineralization effect of nanohydroxyapatite on adjacent glazed surfaces [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 231-235.
[4] Lizhong SUN, Qi WANG, Ruofan WANG, Fanglin MI. Research progress of the influence of sodium hypochlorite deproteinization on the bonding of brackets [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(6): 396-400.
[5] Baoshan LIN, Yuan ZHOU, Biao HU, Ting LI. An in vitro study of the efficacy of tooth liquid polish sealant and Tooth Mousse in prevention of enamel demineralization around the bracket during orthodontic treatment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 565-568.
[6] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560-564.
[7] Jian-zhen YANG, Xiao-qing SHEN, Cheng-xia LIU, Ping-ping XU. The blocking effect and abrasion resistance of hydroxyapatite with different particle sizes and morphologies for dentinal tubules [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(5): 288-293.
[8] Yun LIANG, Ke CHEN. Research progress on the bonding strength of primary teeth after Er, Cr: YSGG laser irradiation [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(3): 196-199.
[9] Jin LEI,Yi WANG,Han-fei ZHANG. Preventive effects of Duraphat on enamel demineralization during orthodontic treatment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(6): 369-371.
[10] Xiao-ling SUN,Peng WANG,Yu-mei CAO,Shu-tai LIU. Comparative study of enamel demineralization of different dairy (drink) goods on the primary teeth in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(10): 574-577.
[11] LIU Fang. Clinical assessment of two fissure sealant techniques in children’s dental caries prevention [J]. journal1, 2016, 24(1): 44-45.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!
This work is licensed under a Creative Commons Attribution 3.0 License.