Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (8): 464-468.doi: 10.12016/j.issn.2096-1456.2016.08.006

• Basic Study • Previous Articles     Next Articles

Study of the cellular growth inhibited by tetrandrine with the expression of β-catenin in oral squamous cell carcinoma cell line

Yan-fei CHENG1(),Yong-rong ZHONG2   

  1. 1. Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510110, China
    2. Guangdong Provincial Stomatological & the Affiliated Stomatological Hospital of Southern Medical Universtity, Guangzhou 510280, China
  • Received:2016-03-15 Revised:2016-04-28 Online:2016-08-20 Published:2016-08-20
  • Contact: Yan-fei CHENG E-mail:feifeicow007@163.com

Abstract:

Objective To study the effect of tetrandrine (TET) on oral squamous cell carcinoma cell line Tca8113 and its influence against the expression of β-catenin.Methods CCK-8 method was used to detect the effect of TET on Tca8113 by different dose and time. Western blot assay was used to detect the expression of β-catenin after treatment by TET meanwhile the distribution of the β-catenin treated by TET was detected by the immunofluorescence method.Results With the increasing dose of TET, the inhibition rate against Tca8113 was raising (P < 0.05). The half maximal inhibitory concentration (IC50) value of TET against Tca8113 was 64 μmol/L. With this concentration, the inhibition rate against Tca8113 exhibited a time-dependent manner. The results of western blot revealed that the expression of β-catenin was significantly inhibited after treatment by TET (P < 0.05). The results of immunofluorescence method showed that the β-catenin protein mainly located at nucleus in Tca8113, while after treatment by TET, the β-catenin protein mainly located at cellular membrane and cytoplasm in a diffused distribution manner.Conclusion Our data suggest that TET can inhibit the cell growth of oral squamous cell carcinoma, and influence the cellular distribution of β-catenin protein in tumor cells.

Key words: Tetrandrine, Oral squamous cell carcinoma, β-catenin, Cell growth, Cell culture

CLC Number: 

  • R739.8

Figure 1

The inhibition rates after treatment by TET for 48 h in different dose against Tca8113 detected by CCK-8 assay"

Figure 2

The influence on inhibition rates against Tca8113 by 64 μmol/L TET in different time detected by CCK-8 assay"

Figure 3

The modulation of β-catenin after treatment by 64 μmol/L TET against Tca8113 detected by western blot assay"

Figure 4

The distribution modulation of β-catenin after treatment by 64 μmol/L TET against Tca8113 detected by immunofluorescence method × 400"

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