Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (9): 557-563.doi: 10.12016/j.issn.2096-1456.2018.09.003

• Basic Study • Previous Articles     Next Articles

Antibacterial activity of the nisin-containing single-bond universal adhesive

Yi TAN,Sui MAI,Jia LIU,Lisha GU()   

  1. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
  • Received:2018-04-15 Revised:2018-05-03 Online:2018-09-20 Published:2018-09-30

RichHTML

22

PDF (PC)

78

Abstract:

Objective To investigate the antibacterial activity to Streptococcus mutans of a nisin-containing single-bond universal adhesive. Methods Nisin was mixed into the bonding agent to produce concentrations ranging from 0.01 g/mL to 0.05 g/mL for the experiments, and adhesive without nisin was used as the control. Dentin-resin specimens were prepared for the microtensile strength test to evaluate changes in the bonding strength. The proper concentrations were selected for more tests. ① An agar diffusion test was applied with filter paper to detect the release of nisin, and adhesive without nisin was used as the negative control, 0.01 g/mL Nisin aqueous solution was used as the positive control. ② Solidification; resin adhesive specimens were prepared for the assessment of direct contact inhibition activity. ③ Confocal laser scanning microscopy was used to examine the effect of the adhesive on the biological film activity and the ability of Streptococcus mutans to produce extracellular polysaccharides. Results Nisin did not significantly reduce the bond strength of the modified adhesive at 0.01-0.03 g/mL (P < 0.05); these concentrations were selected for the subsequent antibiosis experiment. Rings could not be observed in the agar diffusion test, except for in the group of adhesive modified with 0.01-0.03 g/mL nisin. Resin adhesive with 0.01-0.03 g/mL nisin could significantly inhibit the proliferation of Streptococcus mutans on the surface of the specimens. The confocal laser scanning microscopy results indicate that only the adhesive resin modified with nisin could reduce the bacteria in the biofilm and the production of extracellular polysaccharides. Conclusion Single-bond universal adhesive with 0.01-0.03 g/mL nisin can inhibit the growth of Streptococcus mutans and its biofilms on the bonding interface, as well as decrease the production of extracellular polysaccharides, and thus has the potential to decrease the occurrence of secondary caries.

Key words: Nisin, Streptococcus mutans, Biofilm, Extracellular polysaccharides, Resin adhesive

CLC Number: 

  • R781.5

Figure 1

Microtensile strength in each group"

Figure 2

Agar diffusion test results"

Table 1

Colony counts on the adhesive surface after 24 hours"

组别（g/mL） 菌落数（CFU/mL）
对照组 （2.37 ± 2.74） × 106
0.01 g/mL组 （5.63 ± 2.67） × 104 1）
0.02 g/mL组 （5.13 ± 3.12） × 104 1）
0.03 g/mL组 （5.83 ± 1.10） × 104 1）

Figure 3

Dead/live biofilm staining"

Figure 4

Extracellular polysaccharide biofilm staining"

[1] Demarco FF, Correa MB, Cenci MS , et al. Longevity of posterior composite restorations: not only a matter of materials[J]. Dental Mater, 2012,28(1):87-101.
doi: 10.1016/j.dental.2011.09.003 pmid: 22192253
[2] Totiam P, González-Cabezas C, Fontana MR , et al. A new in vitro model to study the relationship of gap size and secondary caries[J]. Caries Res, 2007,41(6):467-473.
doi: 10.1159/000107934 pmid: 17827964
[3] Hollanders ACC, Kuper NK, Opdam NJM , et al. Preventive dentistry 5. Secondary caries[J]. Ned Tijdschr Tandheelkd, 2017,124(5):257-263.
doi: 10.5177/ntvt.2017.05.16212 pmid: 28501880
[4] Bourbia M, Ma D, Cvitkovitch DG , et al. Cariogenic bacteria degrade dental resin composites and adhesives[J]. J Dent Res, 2013,92(11):989-994.
doi: 10.1177/0022034513504436 pmid: 3797536
[5] Imazato S, Ma S, Chen JH , et al. Therapeutic polymers for dental adhesives: loading resins with bio-active components[J]. Dent Mater, 2014,30(1):97-104.
doi: 10.1016/j.dental.2013.06.003 pmid: 4312699
[6] Dutra-Correa M, Leite AABV, de Cara SPHM , et al. Antibacterial effects and cytotoxicity of an adhesive containing low concentration of silver nanoparticles[J]. J Dent, 2018,pii: S0300-5712(18) 30206-9. doi: 10.1016/j.jdent. 2018.07.010.
[7] Yue S, Wu J, Zhang Q , et al. Novel dental adhesive resin with crack self-healing, antimicrobial and remineralization properties[J]. J Dent, 2018,75:48-57.
doi: 10.1016/j.jdent.2018.05.009
[8] Lukomska-Szymanska M, Zarzycka B, Grzegorczyk J , et al. Antibacterial properties of calcium fluoride-based composite materials: in vitro study[J]. Biomed Res Int, 2016,2016:1048320.
doi: 10.1155/2016/1048320
[9] Wang X, Zhang N, Wang B , et al. Novel self-etching and antibacterial orthodontic adhesive containing dimethylaminohexadecyl methacrylate to inhibit enamel demineralization[J]. Dent Mater J, 2018,37(4):555-561.
doi: 10.4012/dmj.2017-286
[10] Ge Y, Wang S, Zhou X , et al. The use of quaternary ammonium to combat dental caries[J]. Materials (Basel), 2015,8(6):3532-3549.
doi: 10.3390/ma8063532 pmid: 4665981
[11] Teixeira Barbosa AA, Mantovani HC, Jain S . Bacteriocins from lactic acid bacteria and their potential in the preservation of fruit products[J]. Crit Rev Biotechnol, 2017,37(7):852-864.
doi: 10.1080/07388551.2016.1262323 pmid: 28049350
[12] Shin JM, Ateia I, Paulus JR , et al. Antimicrobial nisin acts against saliva derived multi-species biofilms without cytotoxicity to human oral cells[J]. Front Microbiol. 2015,6:617.
[13] Moura JS, Lima EM, Paes Leme AF , et al. Effect of luting cement on dental biofilm composition and secondary caries around metallic restorations in situ[J]. Oper Dent, 2004,29(5):509-514.
[14] Buonocore MG . A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces[J]. J Dent Res, 1955,34(6):849-853.
doi: 10.1177/00220345550340060801 pmid: 13271655
[15] Nakabayashi N, Kojima K, Masuhara E . The promotion of adhesion by the infiltration of monomers into tooth substrates[J]. J Biomed Mater Res, 1982,16(3):265-273.
doi: 10.1002/jbm.820160307 pmid: 7085687
[16] Hanabusa M, Mine A, Kuboki T , et al. Bonding effectiveness of a new ′multi-mode′ adhesive to enamel and dentine[J]. J Dent, 2012,40(6):475-484.
doi: 10.1016/j.jdent.2012.02.012 pmid: 22381614
[17] Rayman MK, Aris B, Hurst A . Nisin: a possible alternative or adjunct to nitrite in the preservation of meats[J]. Appl Environ Microbiol, 1981,41(2):375-380.
doi: 10.1007/BF00443249 pmid: 7195188
[18] Pashley DH, Tay FR, Breschi L , et al. State of the art etch-and-rinse adhesives[J]. Dent Mater, 2011,27(1):1-16.
doi: 10.1016/j.dental.2010.10.016 pmid: 21112620
[19] Van Meerbeek B, Yoshihara K, Yoshida Y , et al. State of the art of self-etch adhesives[J]. Dent Mater, 2011,27(1):17-28.
doi: 10.1016/j.dental.2010.10.023 pmid: 21109301
[20] Zhou Y, Millhouse E, Shaw T , et al. Evaluating Streptococcus mutans strain dependent characteristics in a polymicrobial biofilm community[J]. Front Microbiol, 2018,9:1498.
doi: 10.3389/fmicb.2018.01498
[21] Silva TSO, Freitas AR, Pinheiro MLL , et al. Oral biofilm formation on different materials for dental implants[J]. J Vis Exp, 2018, ( 136). doi: 10.3791/57756.
[22] Segev-Zarko L, Saar-Dover R, Brumfeld V , et al. Mechanisms of biofilm inhibition and degradation by antimicrobial peptides[J]. Biochem J, 2015,468(2):259-270.
doi: 10.1042/BJ20141251 pmid: 25761937
[23] Chowdhury A, Nandy SK, Sarkar J , et al. Inhibition of pro-/active MMP-2 by green tea catechins and prediction of their interaction by molecular docking studies[J]. Mol Cell Biochem, 2017,427(1/2):111-122.
[1] REN Lizhi,SUN Rui. New progress in the clinical application of GBR membrane materials [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 404-408.
[2] HE Yuanli,REN Biao,CHEN Xuan,ZOU Ling. Mechanism research of srtA gene on the oxidation tolerance of Streptococcus mutans [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 292-297.
[3] PENG Xinyu,PENG Xian,CHENG Lei. Research progress on the effect of pH-sensitive drug delivery systems on oral microorganisms [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 189-194.
[4] ZHOU Wen,PENG Xian,CHENG Lei. Research progress on factors affecting bacterial adhesion on the oral implant surface [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(2): 102-106.
[5] WANG Zheng,CHENG Lei,ZHOU Xuedong,REN Biao. Research progress on the mechanism of radiocaries formation [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 255-259.
[6] YANG Ting,ZHANG Wanting,LI Beibei,DONG Ying,CAO Hongfei,ZHAO Jin. Distribution of oral Streptococcus mutans and its correlation with dental caries in children of Bortala Mongolian Autonomous Prefecture [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 219-225.
[7] WU Zhengxi,LI Fenglan. Effect of two aging methods on the bonding interface between glass ceramics and dentin [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(11): 703-710.
[8] Ru ZHANG, Lei LEI, Yingming YANG, Tao HU. Regulation mechanism of rnc gene on Streptococcus mutans environmental tolerance and its mechanism [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 504-507.
[9] Yang LIU, Yueping ZHAO. Research advances in the correlations between periodontal disease and autophagy [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(4): 268-272.
[10] ZHOU Qi,LI Jun-xin,ZHONG Bo,ZHANG Xiao-jie,JIANG Shuang-feng. Comparison of the bacterial community structures in root canals with different severity of infection by metagenomic analysis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(9): 519-523.
[11] TIAN Yuan-yuan,YU Xuan,FANG Hong-zhi,YANG Ying-ming,YANG Hui,HU Tao. Effects of exogenous dextranase and sodium fluoride on Actinomyces viscosus biofilm [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(11): 640-644.
[12] Hai-xia LIU,Xuan CHEN,Ling ZOU. An in vitro evaluation of antibacterial properties of 2 pulp capping materials [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(10): 578-581.
[13] YU Pei, XUE Jing, ZHANG Xiao-wei, ZHENG Cang-shang. The influence of the roughness of zirconia ceramic surface on microbial attachment [J]. journal1, 2016, 24(1): 20-25.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Hong-chang LAI,Jun-yu SHI. Maxillary sinus floor elevation[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(1): 8 -12 .
[2] Pin ZHOU, Yang-fei LI. MRI study of temporomandibular joint disc position in asymptomatic volunteers[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(4): 239 -244 .
[3] Xinxin XIA, Fang FANG, Lijuan CHENG. Shaping ability of Pathfile and WaveOne in simulated root canals[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(6): 365 -368 .
[4] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560 -564 .
[5] Chengzhang LI. Masticatory muscles in occlusion[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 755 -760 .
[6] . [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 1 .
[7] Zhirong WU, Shiguang Huang. Research progress on the etiology, clinical examination and treatment of peri-implantitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(6): 401 -405 .
[8] Xiaowu YAO, Shisheng CHEN, Zizheng LU, Minxiao LIN. Clinical report and literature review on the amyloidosis of salivary glands[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 533 -536 .
[9] Lan LIAO, Lijun ZENG. Updated research on digitalization in aesthetic restoration[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 409 -414 .
[10] Yu LU, Chengxia LIU, Zhongjun LIU. Role of TRAF6 in inflammatory responses of human osteoblast-like cells with Enterococcusfaecalis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(7): 420 -425 .

Copyright © Editorial Board of Journal of Prevention and Treatment for Stomatological Diseases

This work is licensed under a Creative Commons Attribution 3.0 License.

Address:Guangzhou City Jiangnan Road South 366, Editorial office ofJournal of Prevention and Treatment for Stomatological Diseases, zip code: 510280　Fax:020-34304115　E-mail：kqjbfz@vip.126.com

Supported by:Beijing Magtech

This work is licensed under a Creative Commons Attribution 3.0 License.