Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (9): 557-563.doi: 10.12016/j.issn.2096-1456.2018.09.003

• Basic Study • Previous Articles     Next Articles

Antibacterial activity of the nisin-containing single-bond universal adhesive

Yi TAN,Sui MAI,Jia LIU,Lisha GU()   

  1. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
  • Received:2018-04-15 Revised:2018-05-03 Online:2018-09-20 Published:2018-09-30

Abstract:

Objective To investigate the antibacterial activity to Streptococcus mutans of a nisin-containing single-bond universal adhesive. Methods Nisin was mixed into the bonding agent to produce concentrations ranging from 0.01 g/mL to 0.05 g/mL for the experiments, and adhesive without nisin was used as the control. Dentin-resin specimens were prepared for the microtensile strength test to evaluate changes in the bonding strength. The proper concentrations were selected for more tests. ① An agar diffusion test was applied with filter paper to detect the release of nisin, and adhesive without nisin was used as the negative control, 0.01 g/mL Nisin aqueous solution was used as the positive control. ② Solidification; resin adhesive specimens were prepared for the assessment of direct contact inhibition activity. ③ Confocal laser scanning microscopy was used to examine the effect of the adhesive on the biological film activity and the ability of Streptococcus mutans to produce extracellular polysaccharides. Results Nisin did not significantly reduce the bond strength of the modified adhesive at 0.01-0.03 g/mL (P < 0.05); these concentrations were selected for the subsequent antibiosis experiment. Rings could not be observed in the agar diffusion test, except for in the group of adhesive modified with 0.01-0.03 g/mL nisin. Resin adhesive with 0.01-0.03 g/mL nisin could significantly inhibit the proliferation of Streptococcus mutans on the surface of the specimens. The confocal laser scanning microscopy results indicate that only the adhesive resin modified with nisin could reduce the bacteria in the biofilm and the production of extracellular polysaccharides. Conclusion Single-bond universal adhesive with 0.01-0.03 g/mL nisin can inhibit the growth of Streptococcus mutans and its biofilms on the bonding interface, as well as decrease the production of extracellular polysaccharides, and thus has the potential to decrease the occurrence of secondary caries.

Key words: Nisin, Streptococcus mutans, Biofilm, Extracellular polysaccharides, Resin adhesive

CLC Number: 

  • R781.5

Figure 1

Microtensile strength in each group"

Figure 2

Agar diffusion test results"

Table 1

Colony counts on the adhesive surface after 24 hours"

组别(g/mL) 菌落数(CFU/mL)
对照组 (2.37 ± 2.74) × 106
0.01 g/mL组 (5.63 ± 2.67) × 104 1)
0.02 g/mL组 (5.13 ± 3.12) × 104 1)
0.03 g/mL组 (5.83 ± 1.10) × 104 1)

Figure 3

Dead/live biofilm staining"

Figure 4

Extracellular polysaccharide biofilm staining"

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