Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (5): 285-291.doi: 10.12016/j.issn.2096-1456.2020.05.003

• Basic Study • Previous Articles     Next Articles

Effect of different doses of aspirin on the early osseointegration of titanium alloy implants in rats

WEN Dandan,LV Yalin()   

  1. Department of Stomatology, Beijing Anzhen hospital, Capital Medical University, Beijing 100029, China
  • Received:2019-12-23 Revised:2020-01-21 Online:2020-05-20 Published:2020-04-24
  • Contact: Yalin LV E-mail:lvyalin@vip.sina.com

Abstract:

Objective To investigate the effects of different doses of aspirin on the early osteointegration of titanium alloy implants in the femurs of rats to provide a reference for dental implantation in patients who take aspirin.Methods Forty-eight 8-week-old SD male rats were randomly divided into four groups, namely, the control, A, B, and C groups. Forty-eight Ti-6Al-4 V implants with a diameter of 1.4 mm and a length of 6 mm were implanted at the distal end of the right femur. In the A, B, and C groups, dosages of aspirin of 8.93 mg/kg/d, 17.86 mg/kg/d, and 26.79 mg/kg/d were administered by gavage starting on the day of surgery, and the control group was given similar doses of 0.9% saline. HE, Masson, BMP-2 immunohistochemical and TRAP staining evaluations were performed in the 2nd and 4th weeks after surgery.Results Compared with the results in the control group, the HE staining results showed that the amount of new bone formation was reduced, the trabeculae were more sparse, and the bone marrow cavity was enlarged around the implants in the B and C groups, with the C group showing the most obvious effects. The Masson staining results showed that, compared with that in the control group, the red-stained area in the new bone tissue around the implant was reduced in groups B and C, and the reduction in group C was more significant. However, there was no significant difference between group A and the control group. The BMP-2 staining results indicated that the expression of BMP-2 was not significantly different between the control group and the A group (P > 0.05), and the expression in group C was lower than that in the other groups (P < 0.05). The TRAP staining results demonstrated that the number of positive cells per unit area was decreased sequentially in the control, A, B, and C groups, and the difference was statistically significant (P < 0.05).Conclusion Aspirin may reduce the formation of bone tissue by inhibiting the activity of osteoblasts and expression in osteoclasts. This effect on osteogenesis was aspirin dose-dependent, and large doses of aspirin can inhibit osteogenesis more significantly.

Key words: aspirin, titanium alloy implants, Sprague-Dawley rat, osteoblast, osteoclast, osteogenesis, bone morphogenetic protein-2, osseointegration

CLC Number: 

  • R78

Figure 1

Results of HE, Masson, BMP-2 and TRAP staining in each group in the 2nd week a, b, c, and d are 10 × microscopic images of HE, Masson, BMP-2, and TRAP staining, respectively Red arrows indicate BMP-2 positive staining areas, blue arrows indicate TRAP positive staining areas, blue rectangles indicate the region of interest, and black rectangles show an enlarged image of TRAP positive staining; the blank area where the green triangle is located indicates where the implant was before it was removed The HE 200 × images show the amount of new bone formation, the sparseness of bone trabeculae, and the gradually decrease in the size of the bone marrow cavity in the control group and the A, B, and C groups; the Masson 200 × images show that the red stained area in the new bone tissue around the implants gradually decreased in size in the control, A, B, and C groups; the BMP-2 200 × images show that the expression of BMP-2 gradually decreased in the control, A, B, and C groups; the TRAP 200 × images show that the number of positive cells per unit area decreased sequentially in the control, A, B, and C group"

Figure 2

Results of HE, Masson, BMP-2 and TRAP staining in each group in the 4th week a, b, c, and d are 10 × microscopic images of HE, Masson, BMP-2, and TRAP staining, respectively Red arrows indicate BMP-2 positive staining areas, blue arrows indicate TRAP positive staining areas, blue rectangles indicate the region of interest, and black rectangles show an enlarged image of TRAP positive staining; the blank area where the green triangle is located indicates where the implant was before it was removed The HE 200 × images show that the amount of new bone formation, the sparseness of the bone trabeculae, and the bone marrow cavity size gradually decreased in the control group and the A, B, and C groups; the Masson 200 × images show that the red stained area in the new bone tissue around the implants was gradually decreased in the control, A, B, and C groups; the BMP-2 200 × images show that the expression of BMP-2 gradually decreased in the control, A, B, and C groups; the TRAP 200 × images show that the number of positive cells per unit area decreased sequentially in the control, A, B, and C groups"

Figure 3

MOD values of BMP-2-positive staining in each group in the 2nd and 4th week a: MOD values of BMP-2-positive staining in each group in the 2nd week; b: MOD values of BMP-2-positive staining in each group in the 4th week; * indicates P < 0.05 compared with the control group; # indicates P < 0.05 compared with the C group"

Figure 4

Amount of TRAP-positive staining per unit area in the 2nd and 4th week *indicates P < 0.05 compared with the other groups in the 2nd and 4th week"

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