Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (4): 222-226.doi: 10.12016/j.issn.2096-1456.2018.04.004

• Basic Study • Previous Articles     Next Articles

Expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells

Wenxi HUANG1(), Ying OUYANG2, Changbo WEI2, Dongsheng YU2()   

  1. 1. Department of Stomatology, Foshan Chancheng District Centre Hospital, Foshan 528000, China
    2. Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
  • Received:2017-10-10 Revised:2017-11-07 Online:2018-04-20 Published:2018-08-31

Abstract:

Objective To assess the expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells. Methods CAL27 tongue squamous cell carcinoma cells and normal human oral keratinocytes (HOKs) were cultured, and then, the cultured supernatant was collected to separate the exosomes. Exosomes were detected by electron microscopy, and the expression levels of miR-21, miR-221, and miR-222 in the exosomes of tongue cancer cells were measured by qRT-PCR. Results Exosomes existed in the cultured supernatants of CAL27 cells and HOKs. Additionally, the expression levels of miR-21, miR-221, and miR-222 in the exosomes of CAL27 cells were significantly enhanced compared with those in the HOK exosomes (P < 0.05). Conclusion The expression levels of miR-21, miR-221, and miR-222 were markedly enhanced in the exosomes of CAL27 tongue squamous cell carcinoma cells.

Key words: Carcinoma, Tongue, Squamous cell, Exosome, miR-21, miR-221, miR-222, qRT-PCR

Table 1

Primer sequences"

miRNA 序列(5′-3′)
H-miR-21-5p-F TAGCTTATCAGACTGATGTT
H-miR221-3p-F AGCTACATTGTCTGCTGGGT
H-miR222-3p-F AGCTACATCTGGCTACTGGGT
H-U6-F CGCTTCGGCAGCACATATACTAA
Universal Primer-R TTACCTAGCGTATCGTTGAC

Figure 1

Exosomes and exosome markers were measured"

Figure 2

The electrophoretogram of total RNA in exosomes"

Figure 3

Amplification curves and melting curves for miR-21, miR-221 and miR-222 expression in exosomes detected by qRT-PCR"

Figure 4

The expression of miR-21, miR-221 and miR-222 in the exosomes of HOKs and CAL27 cells"

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