Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (12): 809-819.DOI: 10.12016/j.issn.2096-1456.2021.12.003

• Basic Study • Previous Articles     Next Articles

Prediction of the pharmacological characteristics and therapeutic mechanism of Tripterygium wilfordii in treating oral lichen planus based on network pharmacology

WU Zeyu1,2(),ZHAO Jin1,2,WANG Chen1,GONG Yi1,XUE Rui2,3()   

  1. 1. Department of Cariology and Endodontics, The First Affiliated Hospital of Xinjiang Medical University (The Affiliated Stomatology Hospital of Xinjiang Medical University), Urumqi 830054, China
    2. Stomatology Disease Institute of Xinjiang Uyghur Autonomous Region, Urumqi 830054, China
    3. Department of Integrated Traditional Chinese Medicine and Oral Medicine, The First Affiliated Hospital of Xinjiang Medical University (The Affiliated Stomatology Hospital of Xinjiang Medical University), Urumqi 830054, China
  • Received:2020-12-05 Revised:2021-06-07 Online:2021-12-20 Published:2021-08-17
  • Contact: Rui XUE
  • Supported by:
    Natural Science Foundation of Xinjiang Province(2016D01C248)

网络药理学探索雷公藤治疗口腔扁平苔藓的药理特性和治疗机制

吴泽钰1,2(),赵今1,2,王琛1,龚怡1,薛瑞2,3()   

  1. 1.新疆医科大学第一附属医院(附属口腔医院)牙体牙髓病科,新疆维吾尔自治区 乌鲁木齐(830054)
    2.新疆维吾尔自治区口腔医学研究所,新疆维吾尔自治区 乌鲁木齐(830054)
    3.新疆医科大学第一附属医院(附属口腔医院)口腔黏膜中西医结合科,新疆维吾尔自治区 乌鲁木齐(830054)
  • 通讯作者: 薛瑞
  • 作者简介:吴泽钰,主治医师,博士,Email: wuzeyuyu@hotmail.com
  • 基金资助:
    新疆维吾尔自治区自然科学基金-联合基金(2016D01C248)

Abstract:

Objective To explore the potential mechanism of the main active component Tripterygium wilfordii in the treatment of oral lichen planus based on network pharmacology. Methods The components of Tripterygium wilfordii and targets were searched through the Traditional Chinese Medicine system pharmacology database and analysis platform (TCMSP) and the Traditional Chinese Medicine integrated database (TCMID) databases. The related targets of oral lichen planus (OLP) were obtained through databases such as Gene Cards. The OLP targets were mapped by Venn analysis to the targets of Tripterygium wilfordii to screen out the common targets as the treatment of OLP targets of Tripterygium wilfordii. The Cytoscape software and STRING were used to construct a chemical component-target network and protein-protein interaction network, a network analyzer was used to compute the network topology properties, a cluster profiler software was used to analyze the GO classification enrichment analysis and KEGG signal path analysis, and a Tripterygium wilfordii chemical components-targets-pathway network diagram was constructed. Results Twenty-three components and 44 OLP treatmenttargets of Tripterygium wilfordii were obtained. The key active ingredients of Tripterygium wilfordii in the treatment of OLP are triptolide, kaempferol, and tangerine peel. The key targets include TNF and AKT1. The GO classification enrichment analysis obtained 63 GO terms, which are mainly involved in the leukocyte differentiation and reaction to lipopolysaccharides. The KEGG analysis identified 111 signaling pathways, which are mainly related to the TNF signaling pathway and IL17 signaling pathway. Conclusion Based on the network pharmacology, this study preliminarily revealed themain components, targets and pathways of Tripterygium wilfordii in the treatment of OLP. This study can provide a theoretical basis for further research to explore drugs with high activity and low toxicity to treat OLP from Tripterygium wilfordii.

Key words: Tripterygium wilfordii, triptolide, kaempferol, network pharmacology, pharmacological characteristics, active ingredient, OLP, target, therapeutic mechanism

摘要:

目的 基于网络药理学方法研究雷公藤治疗口腔扁平苔藓(oral lichen planus,OLP)的主要活性成分及其潜在的药理特性和作用机制。方法 基于中药系统药理学分析平台等获取雷公藤化学成分及靶点,Gene Cards等数据库获取OLP靶点,将药物与疾病靶点进行韦恩分析,得到雷公藤治疗OLP的潜在靶点。采用Cytoscape 3.7.2构建化学成分-靶点网络,STRING构建蛋白质-蛋白质相互作用网络,Network Analyzer计算网络拓扑属性,利用Cluster Profiler软件包进行GO、KEGG富集分析,并构建雷公藤化学成分-靶点-通路网络。结果 获得雷公藤23个成分和150个靶点,OLP靶点472个,交集靶点44个。雷公藤治疗OLP的核心活性成分有雷公藤内酯、山奈酚、川陈皮素等,关键靶点包括肿瘤坏死因子、丝氨酸/苏氨酸蛋白激酶1等。GO富集分析得到白细胞分化、对脂多糖的反应等63个GO条目。KEGG富集分析得到TNF信号通路、IL17信号通路等111条通路。结论 应用网络药理学方法初步揭示雷公藤治疗OLP的主要成分、作用靶点和作用途径,为将来从雷公藤中开发出高活性、低毒性的治疗OLP的药物的深入研究提供了理论依据。

关键词: 雷公藤, 雷公藤内酯, 山奈酚, 网络药理学, 药理特性, 活性成分, 口腔扁平苔藓, 靶点, 治疗机制

CLC Number: