%0 Journal Article %A Jun WEN %A Shuai-mei XU %A Ying LIU %T Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells %D 2016 %R 10.12016/j.issn.2096-1456.2016.08.005 %J Journal of Prevention and Treatment for Stomatological Diseases %P 459-463 %V 24 %N 8 %X

Objective To evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and adipogenic differentiation of dental pulp stem cells (DPSCs).Methods The effect of XAV-939 on the proliferation of DPSCs was analyzed by cell counting kit-8(CCK-8). The effect of XAV-939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of glycogen synthase kinase-3β(GSK3β) and β-catenin which were Wnt signaling marker genes, and peroxisome proliferator activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) which were adipogenic marker genes.Results After 14 days, XAV-939 up-regulates expression of GSK3β、PPARγ2 and C/EBPα, while down-regulates β-catenin.Conclusion XAV-939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt signalling pathway.

%U https://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2016.08.005