口腔疾病防治 ›› 2016, Vol. 24 ›› Issue (4): 218-222.DOI: 10.12016/j.issn.2096-1456.2016.04.004

• 基础研究 • 上一篇    下一篇

柠檬酸致牙釉质脱矿的研究

刘成霞1,杨剑珍1,宁晖丽1,龚涛1,钟素兰1,申晓青2()   

  1. 1. 广东省口腔医院,广东 广州(510280)
    2. 南方医科大学珠江医院口腔科
  • 收稿日期:2015-11-16 修回日期:2015-12-30 出版日期:2016-04-20 发布日期:2016-04-20
  • 通讯作者: 申晓青
  • 作者简介:刘成霞,主治医师,博士, Email: chengxialiu@163.com
  • 基金资助:
    广东省自然科学基金(2014A030313330)

A study on enamel demineralization produced by citric acid

Cheng-xia LIU1,Jian-zhen YANG1,Hui-li NING1,Tao GONG1,Su-lan ZHONG1,Xiao-qing SHEN2()   

  1. 1. Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China
  • Received:2015-11-16 Revised:2015-12-30 Online:2016-04-20 Published:2016-04-20
  • Contact: Xiao-qing SHEN

摘要:

目的 观察10%柠檬酸酸蚀牙釉质不同时间的效果,以获得研究早期釉质龋的可靠体外实验模型,探索更高效的牙釉质脱矿实验方法。方法选取离体人恒前磨牙切割制成牙釉质块,经10%柠檬酸及部分饱和酸缓冲脱矿系统脱矿,扫描电镜观察牙釉质表面经柠檬酸酸蚀不同时间(90 s、120 s、150 s)及部分饱和酸缓冲脱矿系统脱矿1周后的形态学特征,并用显微硬度计检测标本脱矿前后表面显微硬度。结果牙釉质进行脱矿处理后,釉质表面呈现酸蚀状外观。酸蚀90 s时,仅见小部分釉柱晶格脱矿溶解,脱矿牙釉质的能力有限;酸蚀120 s时,与早期自然龋损近似,呈蜂窝状;酸蚀150 s时,牙釉质表面呈酸蚀样破坏形貌,属于过度酸蚀;而部分饱和酸缓冲脱矿系统脱矿效果相对较差。结论柠檬酸具有较强的酸蚀作用,其脱矿牙釉质的能力随着时间的延长而增强,10%的柠檬酸酸蚀120 s可获得早期釉质龋模型。

关键词: 柠檬酸, 早期釉质龋, 牙釉质, 扫描电镜, 脱矿

Abstract:

Objective To observe the effect of different etching time of citric acid on enamel blocks to acquire a reliable in vitro model for early enamel caries, and explore more effective experiment method for enamel demineralization.Methods Human premolars were selected and made into enamel blocks. The morphologies of enamel blocks surfaces etched by 10% citric acid for different time periods (90 s, 120 s, 150 s) and partially saturated acid buffer demineralization system for a week were observed by scanning electron microscope. And the surface micro hardness of these specimen before and after demineralization were tested by the micro hardness tester.Results After demineralization, enamel surface had an obvious acid etching appearance. In the 90 s etching time group, only a small part of enamel prism lattice was demineralized and dissolved, and the ability of demineralization was limited. In 120 s etching time group, the morphology of enamel blocks surface was similar to natural early enamel lesions, like the honeycomb. In the 150 s etching time group, the enamel surface had an acid corrosion damage morphology, and it was excessive etched. However,the demineralization effect of partially saturated acid buffer demineralization system was relatively low.Conclusion Citric acid has a strong etching effect, and its ability of demineralization is enhanced with the extension of time. The model of early enamel lesions can be obtained by 10% citric acid for 120 s.

Key words: Citric acid, Early enamel caries, Enamel, Scanning electron microscope, Demineralization

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