口腔疾病防治 ›› 2022, Vol. 30 ›› Issue (7): 457-463.DOI: 10.12016/j.issn.2096-1456.2022.07.001

• 基础研究 • 上一篇    下一篇

蛋白聚糖与小鼠牙周炎牙槽骨吸收的相关性

王思远(), 张璠, 王雪奎, 孙瑶()   

  1. 上海牙组织修复与再生工程技术研究中心,同济大学口腔医学院,同济大学附属口腔医院种植科,上海(200072)
  • 收稿日期:2021-11-08 修回日期:2021-12-31 出版日期:2022-07-20 发布日期:2022-04-25
  • 通讯作者: 孙瑶
  • 作者简介:王思远,硕士研究生,Email: 907480514@qq.com
  • 基金资助:
    国家自然科学基金项目(81822012);国家重点研发计划项目(2016YFC102705)

Correlation between proteoglycan and alveolar bone resorption in a mouse periodontitis model

WANG Siyuan(), ZHANG Fan, WANG Xuekui, SUN Yao()   

  1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Implantology, School & Hospital of Stomatology, Tongji University, Shanghai 200072, China
  • Received:2021-11-08 Revised:2021-12-31 Online:2022-07-20 Published:2022-04-25
  • Contact: SUN Yao
  • Supported by:
    Natural Science Foundation of China(81822012);National Key R&D Program of China(2016YFC102705)

摘要:

目的 探讨小鼠牙周炎牙槽骨中蛋白聚糖含量的改变及其与牙周炎牙槽骨吸收的相关性。方法 选取12只8周龄C57BL/6J雄性鼠,6-0丝线结扎右侧上颌第二磨牙建立牙周炎模型,左侧上颌未结扎处作为对照,术后14 d处死小鼠。Micro-CT扫描分析牙槽骨吸收情况;HE染色观察牙槽骨形态变化;TRAP染色观察破骨细胞阳性率的改变;RT-qPCR检测细胞外蛋白聚集蛋白聚糖(aggrecan,ACAN)、双链蛋白聚糖(biglycan,BGN)、饰胶蛋白聚糖(decorin,DCN)和多能蛋白聚糖(versican,VCAN)等蛋白聚糖相关基因表达情况,组织蛋白酶K(cathepsin K,CTSK)、基质金属蛋白酶-9(matrix metalloprotein-9,MMP-9)、核因子κB受体活化因子配体(receptor activator of nuclear factor kappa-B ligand,RANKL)等破骨细胞相关基因表达情况,白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)等炎症相关基因表达情况;最后对蛋白聚糖与破骨相关基因表达量,蛋白聚糖与炎症相关基因表达量进行Pearson相关性分析。结果 牙周炎侧牙槽骨吸收增多。TRAP染色结果显示牙周炎侧牙槽骨破骨细胞数量上升。RT-qPCR结果显示牙周炎侧蛋白聚糖相关基因ACAN、BGN、DCN的表达较对照侧降低,VCAN的表达较对照侧升高。牙周炎侧破骨细胞相关基因CTSK、MMP-9、RANKL及炎症相关基因IL-1β、IL-6、TNF-α的表达较对照侧升高(P<0.05)。Pearson相关性分析表明牙周炎中蛋白聚糖与破骨细胞相关基因表达量、炎症相关基因表达量均具有负相关性(P<0.05)。结论 牙周炎时牙槽骨蛋白聚糖的表达与牙槽骨吸收密切相关。

关键词: 牙周炎, 牙周组织, 细胞外基质蛋白, 蛋白聚糖, 糖基化, 破骨细胞, 炎症, 牙槽骨吸收

Abstract:

Objective To analyze changes in proteoglycan and its correlation with alveolar bone resorption in periodontitis. Methods Twelve eight-week-old C57BL/6J male mice were selected, and the periodontitis model was established by ligating the right maxillary second molar with 6-0 silk thread. The nonligated part of the left maxilla was used as the control. The mice were killed 14 days after the operation. Micro-CT was used to assess alveolar bone resorption. HE staining was used to observe the alveolar bone profile, and TRAP staining was conducted to examine the positive rate of osteoclasts. The expression of proteoglycan-related genes, such as aggrecan (ACAN), biglycan (BGN), versican (VCAN), decorin (DCN), osteoclast-related genes, such as cathepsin K (CTSK), matrix metalloprotein-9 (MMP-9), and receptor activator of nuclear factor kappa-B ligand (RANKL), and inflammation-related genes, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), was detected by real-time quantitative PCR. Additionally, the correlation of the expression of proteoglycans with osteoclast-related genes and inflammation-related genes was evaluated by Pearson correlation analysis. Results The resorption of alveolar bone on the periodontitis side increased. TRAP staining showed that the number of osteoclasts was substantially increased in the maxilla with periodontitis. Real-time quantitative PCR demonstrated that compared with the control side, the expression of proteoglycan-related genes, such as ACAN, BGN, and DCN, was decreased, whereas the expression of the VCAN gene was significantly increased in the periodontitis side. Meanwhile, the expression of osteoclast-related genes, such as CTSK, MMP-9, and RANKL, and inflammation-related genes, such as IL-1β, IL-6, and TNF-α, was markedly increased in the periodontitis side (P<0.05). Pearson correlation analysis indicated a negative correlation between the expression of proteoglycans and the mRNA levels of osteoclast-related genes and inflammation-related genes (P<0.05). Conclusion The expression of proteoglycan was closely related to alveolar bone resorption in a periodontitis model.

Key words: periodontitis, periodontal tissue, extracellular matrix protein, proteoglycan, glycosylation, osteoclasts, inflammation, alveolar bone resorption

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