口腔疾病防治 ›› 2022, Vol. 30 ›› Issue (8): 542-548.DOI: 10.12016/j.issn.2096-1456.2022.08.002

• 基础研究 • 上一篇    下一篇

金银花对变异链球菌UA159体外作用的实验研究

曾华倩1,2(), 毛玲1,2, 晋雅恒2, 李适廷1, 徐皑1()   

  1. 1.西南医科大学附属口腔医院,四川 泸州(646000)
    2.西南医科大学口腔医学院,四川 泸州(646000
  • 收稿日期:2022-01-06 修回日期:2022-02-20 出版日期:2022-08-20 发布日期:2022-05-09
  • 通讯作者: 徐皑
  • 作者简介:曾华倩,硕士研究生;Email: 2495094705@qq.com
  • 基金资助:
    四川省科技计划项目(2018JY0399)

In vitro study of the effects of honeysuckle on Streptococcus mutans UA159

ZENG Huaqian1,2(), MAO Ling1,2, JIN Yaheng2, LI Shiting1, XU Ai1()   

  1. 1. The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China
    2. School of Stomatology, Southwest Medical University, Luzhou 646000, China
  • Received:2022-01-06 Revised:2022-02-20 Online:2022-08-20 Published:2022-05-09
  • Contact: XU Ai
  • Supported by:
    Sichuan Province Science and Technology Plan Project(2018JY0399)

摘要:

目的 探讨金银花对变异链球菌UA159的体外抑制作用。方法 通过液体二倍稀释法测定金银花对变异链球菌UA159的最低抑菌浓度(minimium inhibitory concentration,MIC);以溶剂DMSO溶解金银花粉末,配制不同浓度药液,加入菌液,同时设置溶剂对照组与菌液对照组。通过抑菌实验测定金银花对变异链球菌UA159生长及产酸作用,绘制生长曲线和产酸曲线;计算黏附率及黏附抑制率;采用结晶紫定量法测定金银花对变异链球菌UA159生物膜形成量的影响;利用正置显微镜、扫描电子显微镜(scanning electron microscope,SEM)观察生物膜形成情况及细菌数量变化。结果 金银花对变异链球菌UA159的MIC值为12.5 mg/mL;抑菌实验显示金银花对变异链球菌UA159生长、产酸、黏附的抑制作用较对照组强,差异均具有统计学意义(P<0.05),且随着药液浓度增强,抑制作用增加。结晶紫定量法结果显示药液组生物膜形成量与对照组相比下降,差异有统计学意义(P<0.05),同时正置显微镜下显示生物膜形成量明显下降。扫描电镜下,药液组0、6、12 h加入金银花细菌数量均明显下降。结论 金银花能够抑制变异链球菌UA159的生长、产酸,同时也能抑制其黏附及生物膜的形成。

关键词: 金银花, 天然药物, 变异链球菌UA159, 龋病, 细菌生长, 最低抑菌浓度, 细菌产酸, 黏附率, 黏附抑制率, 生物膜

Abstract:

Objective To investigate the inhibitory effect of honeysuckle on Streptococcus mutans UA159 in vitro. Methods We used a double-dilution method to measure the minimum inhibitory concentration (MIC) of honeysuckle against Streptococcus mutans UA159. Lonicerae lonicerae powder was dissolved in the solvent DMSO, different concentrations of liquid medicine were prepared, and bacterial liquid was added. The solution control group and bacterial liquid control group were set at the same time. The growth and acid production of UA159 were determined using antibacterial experiments. A growth curve and acid production curves were drawn, and the adhesion rate and adhesion inhibition rate were calculated. The effect of honeysuckle on the formation of Streptococcus mutans UA159 was determined by crystal violet quantification, and a microscope and a scanning electron microscope were used to observe biofilm formation and structural changes. Results The MIC of honeysuckle against Streptococcus mutans UA159 was 12.5 mg/mL. The bacteriostatic experiments showed a difference in the growth, acid production and adhesion of UA159 after honeysuckle treatment (P<0.05) compared with the controls, and the inhibitory effect increased as the drug liquid concentration increased. Crystal violet quantification showed a significant difference in biofilm formation between the pharmaceutical liquid group and the control group (P<0.05). Meanwhile, the forward microscope showed a significant decrease in biofilm formation. Under SEM, the number of bacteria decreased significantly at 0, 6 and 12 h after honeysuckle addition. Conclusion Honeysuckle inhibits the growth and acid production of UA159 and inhibits adhesion and the formation of biofilms.

Key words: honeysuckle, natural medicine, Streptococcus mutans UA159, caries, growth of bacteria, minimum inhibitory concentration, acid-producing bacteria, adhesion rate, adhesion inhibition rate, biofilm

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