口腔疾病防治 ›› 2021, Vol. 29 ›› Issue (8): 515-522.DOI: 10.12016/j.issn.2096-1456.2021.08.002

• 基础研究 • 上一篇    下一篇

血液环境下Biodentine与MTA的颜色稳定性研究

王怡天1(),吴寅龙2,3,余钒源1,吴凡子1,汪成林1,叶玲1()   

  1. 1.口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院牙体牙髓科,四川 成都(610041)
    2.口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院,四川 成都(610041)
    3.山东大学齐鲁医学院口腔医学院·口腔医院牙体牙髓科 山东省口腔组织再生重点实验室 山东省口腔生物材料与组织再生工程实验室,山东 济南(250000)
  • 收稿日期:2021-01-08 修回日期:2021-03-10 出版日期:2021-08-20 发布日期:2021-05-13
  • 通讯作者: 叶玲
  • 作者简介:王怡天,硕士研究生,Email:wangyitian13098@126.com
  • 基金资助:
    四川省科技计划项目(2019YFS0035);四川大学华西口腔医院探索与研发项目(LCYJ2019-18)

Research on the color stability of Biodentine and MTA within the blood environment

WANG Yitian1(),WU Yinlong2,3,YU Fanyuan1,WU Fanzi1,WANG Chenglin1,YE Ling1()   

  1. 1. State Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases & Department of Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2. State Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    3. Department of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Ji’nan 250000, China
  • Received:2021-01-08 Revised:2021-03-10 Online:2021-08-20 Published:2021-05-13
  • Contact: Ling YE
  • Supported by:
    The Scientific-Plan Program of Sichuan Province(2019YFS0035);The Exploration and Creation Project of West China Hospital of Stomatology, Sichuan University(LCYJ2019-18)

摘要:

目的 比较牙体牙髓治疗中常用的骨水泥材料Biodentine与三氧化矿物凝聚体(mineral trioxide aggregrate,MTA)在与血液接触条件下的颜色稳定性,并探讨其可能的变色原因。方法 制备直径5 mm、高3 mm的Biodentine和MTA圆盘,将每种材料的24个圆盘随机分配到去离子水组和脱纤维羊血组中,浸泡1 d和7 d后进行后续检测。进行各类检测的3个时间点为:圆盘固化脱模后即刻、浸泡1 d和7 d后。检测指标如下:在同样光照环境下对圆盘进行拍照,直观比较两种材料的颜色变化;采用比色仪检测两种材料的色度;借助扫描电镜观察两种材料的表面形态;使用X射线能谱仪测量两种材料各元素含量。结果 肉眼观察到与脱膜后即刻相比,在脱纤维羊血组浸泡1 d后仅MTA在明暗度上发生改变,脱纤维羊血组浸泡7 d后Biodentine与MTA颜色均加深变红,但MTA暗度明显增加。比色仪结果显示脱纤维羊血组MTA的7 d色差值?E(21.257 ± 0.955)大于Biodentine的7 d ?E(5.833 ± 0.501)(t=24.781,P < 0.001);MTA材料自身变色随着与血液接触时间延长而加深,其7 d ?E与1 d ?E(6.233 ± 0.888)相比,差异具有统计学意义(t=19.956,P < 0.001);而Biodentine材料自身变色随时间延长则无统计学意义[7 d ?E与1 d ?E(6.790 ± 0.831)相比,t=1.707,P=0.163]。扫描电镜结果表明:用脱纤维羊血浸泡7 d后,MTA表面孔隙率大于Biodentine,且MTA的晶体边缘相较Biodentine也更为圆钝。X射线能谱仪检测显示:在脱纤维羊血组浸泡7 d后MTA中氧元素含量下降、铋元素含量上升;与此同时Biodentine因阻射能力低未被检测出锆元素,但其他元素含量稳定。结论 Biodentine在与血液接触条件下的颜色稳定性优于MTA,这主要与Biodentine表面孔隙率小、阻射剂成分稳定有关。

关键词: Biodentine, MTA, 颜色稳定性, 牙髓血运重建术, 牙体变色, 骨水泥, 牙髓再生, 材料表征

Abstract:

Objective To compare the color stability of Biodentine and mineral trioxide aggregate (MTA) within the blood environment in vitro and to further investigate the underlying reasons for such color instability. Methods We first generated Biodentine and MTA discs with a diameter of 5 mm and a height of 3 mm. 24 discs of each material were randomly divided into two groups: the deionized water group and the defibrinated sheep blood group. Discs of each group were immersed for 1 day or 7 days before assessments. First, all discs were photographed to directly compare the discoloration of Biodentine and MTA. The color degree of the two materials was tested by a spectrophotometer. Then, the high-resolution morphological characteristics were observed by scanning electron microscopy. Finally, the chemical contents of each element in the material were measured by energy-dispersive spectroscopy. Results Compared to immediately after stripping, a change in the brightness of discs after immersion in defibrinated sheep blood for 1 day was observed only in MTA. On the 7th day after being immersed in blood, the colors of both the Biodentine and MTA discs darkened and turned deep red, but the darkness of the MTA discs increased significantly. The color change of MTA immersed in blood was measured on a spectrophotometer with a greater 7-day ?E (21.257 ± 0.955) than the Biodentine 7-day ?E (5.833 ± 0.501) (t=24.781, P < 0.001). MTA exhibits more discoloration as the immersion time goes on. A significant difference was noted between the 1-day ?E(6.233 ± 0.888) and the 7-day ?E(t=19.956, P < 0.001) of MTA immersed in blood. However, there was no statistically significant difference between the 1-day ?E (6.790 ± 0.831) and the 7-day ?E(t=1.707, P=0.163) of Biodentine immersed in blood. It was observed by scanning electron microscopy that after 7 days of immersion in the defibrinated sheep ablood, the surface porosity of MTA was larger than that of Biodentine, and the crystal edge of MTA became rounded and blunt. The analysis by energy-dispersive X-ray spectroscopy showed that the oxygen content decreased and the bismuth content increased in MTA after immersion in defibrinated sheep blood for 7 days. Zirconium was not detected in Biodentine due to its low radiodensity, but the contents of other elements were stable in Biodentine after immersion in defibrinated sheep blood for 7 days. Conclusion The color stability of Biodentine within the blood environment is better than that of MTA in vitro, which is mainly related to the low surface porosity and stable composition of the anti-radiation agent of Biodentine.

Key words: Biodentine, MTA, color stability, pulp revascularization, tooth discoloration, bone cement, dental pulp regeneration, material characterization

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