血液环境下Biodentine与MTA的颜色稳定性研究

doi:10.12016/j.issn.2096-1456.2021.08.002

口腔疾病防治 ›› 2021, Vol. 29 ›› Issue (8): 515-522.DOI: 10.12016/j.issn.2096-1456.2021.08.002

血液环境下Biodentine与MTA的颜色稳定性研究

王怡天¹(),吴寅龙^2,³,余钒源¹,吴凡子¹,汪成林¹,叶玲¹()

1.口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院牙体牙髓科,四川成都（610041）
2.口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院,四川成都（610041）
3.山东大学齐鲁医学院口腔医学院·口腔医院牙体牙髓科山东省口腔组织再生重点实验室山东省口腔生物材料与组织再生工程实验室,山东济南（250000）

收稿日期:2021-01-08 修回日期:2021-03-10 出版日期:2021-08-20 发布日期:2021-05-13
通讯作者: 叶玲
作者简介:王怡天,硕士研究生,Email:wangyitian13098@126.com
基金资助:
四川省科技计划项目(2019YFS0035);四川大学华西口腔医院探索与研发项目(LCYJ2019-18)

Research on the color stability of Biodentine and MTA within the blood environment

WANG Yitian¹(),WU Yinlong^2,³,YU Fanyuan¹,WU Fanzi¹,WANG Chenglin¹,YE Ling¹()

1. State Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases & Department of Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
2. State Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
3. Department of Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Ji’nan 250000, China

Received:2021-01-08 Revised:2021-03-10 Online:2021-08-20 Published:2021-05-13
Contact: Ling YE
Supported by:
The Scientific-Plan Program of Sichuan Province(2019YFS0035);The Exploration and Creation Project of West China Hospital of Stomatology, Sichuan University(LCYJ2019-18)

摘要/Abstract

摘要：

目的比较牙体牙髓治疗中常用的骨水泥材料Biodentine与三氧化矿物凝聚体（mineral trioxide aggregrate,MTA）在与血液接触条件下的颜色稳定性,并探讨其可能的变色原因。方法制备直径5 mm、高3 mm的Biodentine和MTA圆盘,将每种材料的24个圆盘随机分配到去离子水组和脱纤维羊血组中,浸泡1 d和7 d后进行后续检测。进行各类检测的3个时间点为：圆盘固化脱模后即刻、浸泡1 d和7 d后。检测指标如下：在同样光照环境下对圆盘进行拍照,直观比较两种材料的颜色变化;采用比色仪检测两种材料的色度;借助扫描电镜观察两种材料的表面形态;使用X射线能谱仪测量两种材料各元素含量。结果肉眼观察到与脱膜后即刻相比,在脱纤维羊血组浸泡1 d后仅MTA在明暗度上发生改变,脱纤维羊血组浸泡7 d后Biodentine与MTA颜色均加深变红,但MTA暗度明显增加。比色仪结果显示脱纤维羊血组MTA的7 d色差值?E（21.257 ± 0.955）大于Biodentine的7 d ?E（5.833 ± 0.501）（t=24.781,P < 0.001）;MTA材料自身变色随着与血液接触时间延长而加深,其7 d ?E与1 d ?E（6.233 ± 0.888）相比,差异具有统计学意义（t=19.956,P < 0.001）;而Biodentine材料自身变色随时间延长则无统计学意义[7 d ?E与1 d ?E（6.790 ± 0.831）相比,t=1.707,P=0.163]。扫描电镜结果表明：用脱纤维羊血浸泡7 d后,MTA表面孔隙率大于Biodentine,且MTA的晶体边缘相较Biodentine也更为圆钝。X射线能谱仪检测显示：在脱纤维羊血组浸泡7 d后MTA中氧元素含量下降、铋元素含量上升;与此同时Biodentine因阻射能力低未被检测出锆元素,但其他元素含量稳定。结论 Biodentine在与血液接触条件下的颜色稳定性优于MTA,这主要与Biodentine表面孔隙率小、阻射剂成分稳定有关。

关键词: Biodentine, MTA, 颜色稳定性, 牙髓血运重建术, 牙体变色, 骨水泥, 牙髓再生, 材料表征

Abstract:

Objective To compare the color stability of Biodentine and mineral trioxide aggregate (MTA) within the blood environment in vitro and to further investigate the underlying reasons for such color instability. Methods We first generated Biodentine and MTA discs with a diameter of 5 mm and a height of 3 mm. 24 discs of each material were randomly divided into two groups: the deionized water group and the defibrinated sheep blood group. Discs of each group were immersed for 1 day or 7 days before assessments. First, all discs were photographed to directly compare the discoloration of Biodentine and MTA. The color degree of the two materials was tested by a spectrophotometer. Then, the high-resolution morphological characteristics were observed by scanning electron microscopy. Finally, the chemical contents of each element in the material were measured by energy-dispersive spectroscopy. Results Compared to immediately after stripping, a change in the brightness of discs after immersion in defibrinated sheep blood for 1 day was observed only in MTA. On the 7th day after being immersed in blood, the colors of both the Biodentine and MTA discs darkened and turned deep red, but the darkness of the MTA discs increased significantly. The color change of MTA immersed in blood was measured on a spectrophotometer with a greater 7-day ?E (21.257 ± 0.955) than the Biodentine 7-day ?E (5.833 ± 0.501) (t=24.781, P < 0.001). MTA exhibits more discoloration as the immersion time goes on. A significant difference was noted between the 1-day ?E(6.233 ± 0.888) and the 7-day ?E(t=19.956, P < 0.001) of MTA immersed in blood. However, there was no statistically significant difference between the 1-day ?E (6.790 ± 0.831) and the 7-day ?E(t=1.707, P=0.163) of Biodentine immersed in blood. It was observed by scanning electron microscopy that after 7 days of immersion in the defibrinated sheep ablood, the surface porosity of MTA was larger than that of Biodentine, and the crystal edge of MTA became rounded and blunt. The analysis by energy-dispersive X-ray spectroscopy showed that the oxygen content decreased and the bismuth content increased in MTA after immersion in defibrinated sheep blood for 7 days. Zirconium was not detected in Biodentine due to its low radiodensity, but the contents of other elements were stable in Biodentine after immersion in defibrinated sheep blood for 7 days. Conclusion The color stability of Biodentine within the blood environment is better than that of MTA in vitro, which is mainly related to the low surface porosity and stable composition of the anti-radiation agent of Biodentine.

Key words: Biodentine, MTA, color stability, pulp revascularization, tooth discoloration, bone cement, dental pulp regeneration, material characterization

中图分类号:

王怡天,吴寅龙,余钒源,吴凡子,汪成林,叶玲. 血液环境下Biodentine与MTA的颜色稳定性研究[J]. 口腔疾病防治, 2021, 29(8): 515-522.

WANG Yitian,WU Yinlong,YU Fanyuan,WU Fanzi,WANG Chenglin,YE Ling. Research on the color stability of Biodentine and MTA within the blood environment[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 515-522.

图/表 6

表1 两种材料的基本颜色和组成

Table 1 Basic color and composition of the two materials

Material	Basic color	Composition
MTA	Gray	Tricalcium silicate, dicalcium silicate, tricalcium aluminate, tetracalcium aluminoferrite, bismuth oxide
Biodentine	Primrose yellow	Powder: tricalcium silicate, dicalcium silicate, calcium carbonate, calcium oxide, zirconium oxide, ferric oxide Liquid: calcium chloride, water-soluble polymer

图1 Biodentine和MTA圆盘浸于去离子水或脱纤维羊血中不同时间的照片

Figure 1 Photographs of Biodentine and MTA discs immersed in deionized water or defibrinated sheep blood at different time points Photographs of Biodentine and MTA discs after different treatments were arrayed as shown. The color of the MTA disc immersed in deionized water for 7 days darkened and turned yellow. After immersion in defibrinated sheep blood for 1 day, only the MTA disc displayed a decline in brightness. On the 7th day after being immersed in blood, the colors of both the Biodentine and MTA discs darkened and turned deep red, but the darkness of the MTA discs increased significantly. MTA： mineral trioxide aggregate

表2 Biodentine和MTA圆盘浸于去离子水或脱纤维羊血中不同时间的色差值ΔE

Table 2 ΔE of Biodentine and MTA discs immersed in deionized water or defibrinated sheep blood for 1 day or 7 days $\overline x$ ± s

	Deionized water 1 d	Deionized water 7 d	t	P	Defibrinated sheep blood 1 d	Defibrinated sheep blood 7 d	t	P
Biodentine	2.937 ± 0.099	4.063 ± 0.450	4.239	0.013	6.790 ± 0.831	5.833 ± 0.501	1.707	0.163
MTA	6.163 ± 0.241	13.427 ± 0.926	13.145	< 0.001	6.233 ± 0.888	21.257 ± 0.955	19.956	< 0.001
t	21.437	15.752			0.793	24.781
P	< 0.001	< 0.001			0.472	< 0.001

图2 Biodentine和MTA浸于去离子水或脱纤维羊血中7 d的ΔL、Δa、Δb比较

Figure 2 Comparison of ΔL, Δa and Δb of Biodentine and MTA immersed in deionized water or defibrinated sheep blood for 7 days a： ΔL,Δa and Δb of Biodentine and MTA immersed in deionized water for 7 days; b： ΔL, Δa and Δb of Biodentine and MTA immersed in defibrinated sheep blood for 7 days. MTA： mineral trioxide aggregate; ***: P < 0.001 vs. MTA

图3 扫描电镜检测浸于去离子水或脱纤维羊血中不同时间的Biodentine和MTA的形态学特征

Figure 3 Morphological characteristics of Biodentine and MTA discs immersed in deionized water or defibrinated sheep blood at different time points detected by scanning electron microscopy The surface porosity of MTA was significantly larger than that of Biodentine at each time point in both liquid environments. MTA： mineral trioxide aggregate. Scale plate： 10 μm

图4 脱纤维羊血组浸泡7 d的Biodentine、MTA与未处理组元素含量测定结果

Figure 4 Results of elemental content determination of Biodentine and MTA discs immersed in defibrinated sheep blood for 7 days compared with untreated discs a： EDS results of Biodentine and MTA discs immersed in defibrinated sheep blood for 7 days with untreated discs as controls; b： atomic ratio of oxygen and bismuth in untreated MTA discs and MTA discs immersed in defibrinated sheep blood for 7 days, there was a statistically significant difference between untreated MTA discs and MTA discs immersed in defibrinated sheep blood for 7 days. EDS： energy dispersive spectroscopy; MTA： mineral trioxide aggregation. *： P < 0.05

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血液环境下Biodentine与MTA的颜色稳定性研究

Research on the color stability of Biodentine and MTA within the blood environment

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