口腔疾病防治 ›› 2021, Vol. 29 ›› Issue (11): 746-751.doi: 10.12016/j.issn.2096-1456.2021.11.004

• 基础研究 • 上一篇    下一篇

中老年2型糖尿病牙周炎患者龈沟液抗菌肽水平及其与牙周临床指标的相关性

张雅萌1(),张惠媛1,阮世红2,陈晓春2,甘雪琦1,于海洋1()   

  1. 1.口腔疾病研究国家重点实验室 国家口腔疾病临床研究中心 四川大学华西口腔医院,四川 成都(610041)
    2.深圳市慢性病防治中心,广东 深圳(518000)
  • 收稿日期:2021-03-12 修回日期:2021-05-07 出版日期:2021-11-20 发布日期:2021-07-20
  • 通讯作者: 于海洋 E-mail:1287660531@qq.com;yhyang6812@scu.edu.cn
  • 作者简介:张雅萌,硕士,Email: 1287660531@qq.com
  • 基金资助:
    十三五国家重点研发计划项目(2016YFC1102704)

The level of antimicrobial peptides in gingival crevicular fluid and its correlation with periodontal clinical indexes in elderly patients with type 2 diabetic periodontitis

ZHANG Yameng1(),ZHANG Huiyuan1,RUAN Shihong2,CHEN Xiaochun2,GAN Xueqi1,YU Haiyang1()   

  1. 1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2. Shenzhen Center for Chronic Disease Control, Shenzhen 518000, China
  • Received:2021-03-12 Revised:2021-05-07 Online:2021-11-20 Published:2021-07-20
  • Contact: Haiyang YU E-mail:1287660531@qq.com;yhyang6812@scu.edu.cn
  • Supported by:
    13th Five Year Plan for National Key R&D Program of China(2016YFC1102704)

摘要:

目的 探究牙周炎合并2型糖尿病(type 2 diabetes mellitus, T2DM)患者龈沟液中抗菌肽人-β-防御素-2(human beta-defensin-2, HBD-2)和LL-37的水平变化规律及其与牙周临床指标的相关性。方法 选取于深圳市慢性病防治中心口腔科和内科就诊的中老年人(45至85岁)为研究对象,分为全身健康并牙周健康对照组22人、全身健康牙周炎组19人、T2DM牙周健康组15人、T2DM合并牙周炎组21人。采用佛罗里达牙周探针进行牙周检查,记录受试者牙周临床指标,包括探诊深度(probing depth, PD)、附着水平(attachment level, CAL)和探诊出血(probing bleeding, BOP)。采用酶联免疫吸附法检测龈沟液中抗菌肽HBD-2、LL-37浓度,比较组间抗菌肽HBD-2、抗菌肽LL-37及牙周临床指标间的差异,并进行相关性分析。结果 T2DM合并牙周炎组的PD值大于全身健康牙周炎组(P < 0.05);全身健康牙周炎组龈沟液中HBD-2和抗菌肽LL-37水平均高于全身健康并牙周健康对照组( P< 0.05),全身健康牙周炎组龈沟液中HBD-2水平高于T2DM合并牙周炎组(P< 0.05)。全身健康牙周炎组龈沟液中抗菌肽HBD-2、LL-37与PD、CAL呈正相关(P< 0.05),T2DM合并牙周炎组龈沟液中抗菌肽HBD-2、LL-37与PD、CAL相关性无统计学意义(P > 0.05)。 结论 中老年T2DM牙周炎患者龈沟液抗菌肽HBD-2、LL-37水平较低,与牙周临床指标PD、CAL无显著相关性。

关键词: 中老年; 2型糖尿病; 牙周炎; 龈沟液; 抗菌肽; LL-37; 人-β-防御素-2; 探诊深度; 附着水平; 探诊出血

Abstract:

Objective To investigate the changes and significance of human beta-defensin-2 (HBD-2) and LL-37 in the gingival crevicular fluid of patients with periodontitis and type 2 diabetes mellitus (T2DM). Methods This study was conducted among 45- to 85-year-old patients in the Department of Stomatology and Internal Medicine of Shenzhen Center for Chronic Disease Control, including a healthy control group of 22 people, a systemically healthy control group of 19 people with periodontitis, a T2DM periodontal health group of 15 people, and a T2DM group of 21 people with periodontitis. The Florida periodontal probe was used for periodontal examination, and the clinical indexes, including probing depth (PD), clinical attachment level (CAL) and probing on bleeding (BOP), were recorded. The concentrations of HBD-2 and Ll-37 in gingival crevicular fluid were determined by ELISA. The differences in HBD-2, LL-37 and periodontal clinical indexes between the groups were compared, and correlation analysis was conducted.Results The PD values in T2DM with the periodontitis group were higher than those of the systemically healthy controls with periodontitis group (P < 0.05); the levels of HBD-2 and LL-37 in gingival crevicular fluid in systemically healthy controls with periodontitis group were significantly higher than those in the healthy control group (P < 0.05), the level of HBD-2 in gingival crevicular fluid in systemically healthy controls with periodontitis group was significantly higher than that in T2DM with periodontitis group (P < 0.05); and the antimicrobial peptides HBD-2 and LL-37 in gingival crevicular fluid were significantly positively correlated with the PD and CAL in systemically healthy controls with periodontitis group (P < 0.05), and there was no significant correlation between the antimicrobial peptides HBD-2, LL-37 in gingival crevicular fluid and PD, CAL in T2DM with periodontitis group (P > 0.05). Conclusion The levels of antimicrobial peptides HBD-2 and LL-37 in gingival crevicular fluid of middle-aged and elderly patients with T2DM periodontitis were lower, and there was no significant correlation with PD and CAL in periodontal clinical indicators.

Key words: middle-aged and elderly; type 2 diabetes mellitus; periodontitis; gingival crevicular fluid; antimicrobial peptides; LL-37; human beta defensins-2; probing depth; attachment level; probing bleeding

中图分类号: 

  • R78