Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (2): 79-86.doi: 10.12016/j.issn.2096-1456.2016.02.003

Special Issue: 11

• Basic Study • Previous Articles     Next Articles

Preparation and characterization of genipin-crosslinked silk fibroin/chitosan sustained release microspheres

Man-wen YE1,2,Wei FANG2,Yong SHI2,Jie PAN2,Shu-guang ZENG2()   

  1. 1Department of Stomatology, Guangdong Provincial Maternity and Child Care Center, Guangzhou 510220, China
  • Received:2015-09-02 Revised:2015-10-11 Online:2016-02-20 Published:2016-02-20
  • Contact: Shu-guang ZENG E-mail:sunrisezdoctor@163.com

Abstract:

Objective To investigate the efficiency of different concentrations of genipin and silk fibroin (SF)∶ chitosan (CS) ratios on the package and release of SF-CS composite microspheres. Methods Selected the microspheres with a SF∶CS ratio of 1∶1 to encapsulate different concentrations of bovine serum albumin (BSA) and compared their encapsulation efficiency and sustained-release rate with those of pure chitosan microspheres. SF-CS microspheres were prepared with emulsion cross-linking technique. The microspheres were observed by scanning electron microscope. Size distribution was measured by a laser particle size analyzer. X-ray diffractometry (XRD), fourier transform infrared spectroscopy (FTIR) and thermogravimetricanalyzer (TGA) were used to analyze their structural characteristics. BCA method was used for determination of the drug entrapment, loading rate and cumulative release of the total drug. Results The five types of microspheres (m (CS): m (SF) = 1∶0.5, 0.1 g or 0.5 g genipin; m (CS)∶m (SF) = 1∶1, 0.05 g or 1 g genipin; m (CS)∶m (SF) = 1∶2, 0.5 g eniping) had a more spherical shape and smooth surface with particle size between 70-147 μm. The microspheres prepared with m (CS)∶m (SF) of 1∶1 and 0.05 g genipin in the presence of 10 mg,20 mg, or 50 mg BSA of BSA burst-released (30.79±3.43)%, (34.41±4.46)%, or (41.75±0.96)% of the entrapped BSA on the first day, and cumulatively released (75.20±2.52)%, (79.16±4.31)%, and (89.04±4.68)% in 21 d, respectively. The pure CS microspheres prepared in the presence of 10 mg BSA burst-released (39.53±1.76)% on the first day and cumulatively released (83.57±2.33)% of the total encapsulated BSA in 21 d. Conclusion The SF-CS composite microspheres had a higher sustained release rate than the pure CS microspheres and may be a better drug carrier.

Key words: Silk fibroin, Chitosan, Genipin, Microspheres, Control release

CLC Number: 

  • R988.2

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京尼平(g) m(CS)∶m(SF)
1∶0.5 1∶1 1∶2
0.01 GM-1 GM-6 GM-11
0.05 GM-2 GM-7 GM-12
0.1 GM-3 GM-8 GM-13
0.5 GM-4 GM-9 GM-14
1.0 GM-5 GM-10 GM-15

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组别 粒径(μm) 跨距 平衡水含量(%)
GM-10 71.13±0.85 1.72 60.10±0.48
GM-7 146.60±3.46 2.89 63.34±1.29
GM-14 74.09±1.07 3.98 58.33±0.35
GM-4 89.00±1.08 2.01 58.67±1.45
GM-3 109.83±2.28 3.17 59.60±2.19
F 719.449 6.662
P <0.001 0.007

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组别 BSA (mg) 包封率 (%) 载药率 (%)
SF-CS微球 10 50.16±4.32 1.25±0.11
SF-CS微球 20 56.58±3.58 2.83±0.18
SF-CS微球 50 42.19±7.47 5.27±0.93
CS微球 10 52.77±1.26 2.64±0.06

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