Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (6): 336-340.doi: 10.12016/j.issn.2096-1456.2016.06.004

• Basic Study • Previous Articles     Next Articles

Influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma

Li-na YU1(),Wen-xuan ZHANG1,Yang YU2,Wen-guang QIN1,Xue-qi GUO1   

  1. 1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China
  • Received:2016-02-15 Revised:2016-03-25 Online:2016-06-20 Published:2016-06-20
  • Contact: Li-na YU E-mail:yulina2010@hotmail.com

Abstract:

Objective To investigate the expression of LASP-1 in tongue squamous cell carcinoma, and to study the influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma.Methods Green fluorescence shRNA targeting LASP-1 was constructed and transfected into SCC9 cells by lipofectamine. Cell proliferation was detected by MTT method. LASP-1 mRNA and protein were determined by RT-PCR and western blot respectively. The migration ability of cells was detected by transwell assay.Results There was green fluorescence expression in experimental and negative control group cells after transfecting shRNA and shNC respectively. LASP-1 mRNA and protein were decrease in experimental group cells. It indicated that LASP-1 shRNA was transfected successfully. By contrast with control group, the cell viability of experimental group cells reduced by (51.23 ± 1.47)% and (50.07 ± 2.11)% after 48 h and 72 h respectively. The results of transwell assay showed that migration ability of SCC9 cells was decreased significantly after shRNA targeting LASP-1 was successful transfected. It decreased by 43% compared with control group.Conclusion LASP-1 has high expression in tongue squamous cell carcinoma cells, down regulated LASP-1 gene expression can inhibit the proliferation and migration of SCC9 cells.

Key words: LASP-1, shRNA, Tongue squamous cell carcinoma, Cell proliferation, Migration

CLC Number: 

  • R739.86
[1] Jemal A, Siegel R, Ward E , et al. Cancer statistics, 2007[J]. CA Cancer J Clin, 2007,57(1):43-66.
doi: 10.3322/canjclin.57.1.43
[2] 牛艳红, 周容, 方喜泉 . Yes相关蛋白在舌鳞癌组织中的表达及其意义[J]. 广东牙病防治, 2015,23(6):288-292.
[3] Grunewald GP, Butt E . The LIM and SH3 domain protein family: structural proteins or signal transducers or both[J]. Mol Cancer, 2008,7(7):1-12.
doi: 10.1186/1476-4598-7-31 pmid: 2359764
[4] Raman D, Sai J, Neel NF , et al. LIM and SH3 protein-1 modulates CXCR2-mediated cell migration[J]. PLoS One, 2010,5(4):10050.
doi: 10.1371/journal.pone.0010050 pmid: 2856662
[5] Spence HJ , McGarry L, Chew CS, et al. AP-1 Differentially expressed proteins krp1 and fibronectin cooperatively enhance Rho-ROCK-independent mesenchymal invasion by altering the function, localization, and activity of nondifferentially expressed proteins[J]. Mol Cell Biol, 2006,26(4):1480-1495.
doi: 10.1128/MCB.26.4.1480-1495.2006 pmid: 16449658
[6] Lin YH, Park ZY, Lin D , et al. Regulation of cell migration and survival by focal adhesion targeting of Lasp-1[J]. J Cell Biol, 2004,165(3):421-432.
doi: 10.1083/jcb.200311045 pmid: 15138294
[7] Grunewald TG, Kammerer U, Winkler C , et al. Overexpression of LASP-1 mediates migration and proliferation of human ovarian cancer cells and influences zyxin localization[J]. Br J Cancer, 2007,96(2):296-305.
doi: 10.1038/sj.bjc.6603545 pmid: 17211471
[8] Chiyomaru T, Enokida H, Kawakami K , et al. Functional role of LASP1 in cell viability and its regulation by micro RNAs in bladder cancer[J]. Urol Oncol, 2012,30(4):434-443.
doi: 10.1016/j.urolonc.2010.05.008 pmid: 20843712
[9] Traenka C, Remke M, Korshunov A , et al. Role of LIM and SH3 protein 1 (LASP1) in the metastatic dissemination of medulloblastoma[J]. Cancer res, 2010,70(20):8003-8014.
doi: 10.1158/0008-5472.CAN-10-0592
[10] Wang H, Li W, Jin X , et al. LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma[J]. Eur J cancer, 2013,49(4):974-983.
doi: 10.1016/j.ejca.2012.09.032 pmid: 23084841
[11] Grunewald TG, Kammerer U, Schulze E , et al. Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells[J]. Exp Cell Res, 2006,312(7):974-982.
doi: 10.1016/j.yexcr.2005.12.016 pmid: 16430883
[12] Nakagawa H, Terasaki AG, Suzuki H . Short-term retention of actin filament binding proteins on lamellipodial actin bundles[J]. FEBS Lett, 2006,580(13):3223-3228.
doi: 10.1016/j.febslet.2006.04.082 pmid: 16697374
[13] Panaviene Z, Moncman CL . Linker region of nebulin family members plays an important role in targeting these molecules to cellular structures[J]. Cell Tissue Res, 2007,327(2):353-369.
[14] Mihlan S, Reiß C, Thalheimer P , et al. Nuclear import of LASP-1 is regulated by phosphorylation and dynamic protein-protein interactions[J]. Oncogene, 2013,32(16):2107-2113.
doi: 10.1038/onc.2012.216 pmid: 22665060
[15] Hailer A, Grunewald TG, Orth M , et al. Loss of tumor suppressor mir-203 mediates overexpression of LIM and SH3 Protein 1
( LASP1) in high-risk prostate cancer thereby increasing cell proliferation and migration[J]. Oncotarget, 2014,5(12):4144-4153.
[16] Zheng J, Yu S, Qiao Y , et al. LASP-1 promotes tumor proliferation and metastasis and is an independent unfavorable prognostic factor in gastric cancer[J]. J Cancer Res Clin Oncol, 2014,140(11):1891-1899.
doi: 10.1007/s00432-014-1759-3 pmid: 24990592
[17] Zheng J, Wang F, Lu S , et al. LASP-1, regulated by miR-203, promotes tumor proliferation and aggressiveness in human non-small cell lung cancer[J]. Exp Mol Pathol, 2016,100(1):116-124.
doi: 10.1016/j.yexmp.2015.11.031 pmid: 26683818
[18] Li Z, Chen Y, Wang X , et al. LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway[J]. Cancer Lett, 2016,372(2):239-250.
doi: 10.1016/j.canlet.2016.01.008 pmid: 26797416
[1] HE Jialin, XU Yan, XIE Xianzhe, WANG Tengfei, HUO Dongmei. Effect of platelet-rich fibrin extract on the proliferation of gingival fibroblasts [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(8): 490-495.
[2] Xinyu LIN,Weixiong CHEN,Xinyuan LEI,Zhanpeng OU,song FAN,Jinsong LI. Screening and identification of mitochondrial miRNAs related to chemotherapy resistance in tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 417-422.
[3] Xinyuan LEI,Xinyu LIN,Zhanpeng OU,Yi RUAN,Jinsong LI. Mitochondrial fission protein 1 determines mitochondrial fission and cisplatin sensitivity in tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(6): 350-354.
[4] XU Shuaimei,ZENG Xiongqun,YUAN Peiyan,LIU Zhongjun,ZENG Shuguang. The role of APOD in the proliferation and migration of human dental pulp cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(6): 355-359.
[5] ZHONG Wende,LIU Rongjing,GUAN Hongbing,CHEN Guangsheng. Research on the inhibitory effect of Baicalin on the human tongue squamous cell carcinoma cell line SCC15 [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 226-230.
[6] Xiaojin WANG, Xinchao YOU, Kai CHEN, Kunsong HUANG, Xuan PAN. Influence of luteolin on the invasion and migration of an human tongue squamous carcinoma cell line [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 434-439.
[7] Chaobin PAN. Research progress in comprehensive and sequential treatment of tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(5): 273-280.
[8] Lijuan ZHOU, Siming XIE. Progress in research on connexin 43 in oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(5): 338-340.
[9] Songhao YIN, Weihong GUO. Investigation of the expression of TPX2 in tongue squamous cell carcinoma and its effect on Cal27 cell proliferation and apoptosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 31-37.
[10] Ya-nan WANG, Hao LIU, Jun ZHANG. Expression of autophagy-related proteins PTEN and MAP1LC3 in tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(5): 294-299.
[11] Jun WEN,Shuai-mei XU,Ying LIU. Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(8): 459-463.
[12] Xiao-zhi LÜ,Ting-ru SHAO,Meng ZHAO. Reconstruction of defects with supraclavicular artery island flap following ablation of tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(7): 386-389.
[13] Qian-qian HAN,Zhao LIU,Li JIANG,Hui-yi TANG,Xiao-na LI. Effects of LMK-235 on osteoblast/odontoblast differentiation in hPDLCs [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(7): 390-394.
[14] Zhi-li ZHANG,Wen-hui LU,Wei ZENG,Zhang DENG,Bin ZHOU,You-yuan WANG. Effect of Ezrin on epithelial-to-mesenchymal transition in human tongue squamous cell carcinoma with cisplatin-resistance [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(12): 688-694.
[15] OUYANG Ke-xiong1, LIANG Jun, ZOU Rui, LI Zhi-qiang, BAI Zhi-bao, PIAO Zheng-guo, ZHAO Jian-Jiang.. Ion Torrent RNA-Seq detection and analysis of the long non-coding RNA in tongue squamous cell carcinoma [J]. journal1, 2016, 24(1): 15-19.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!
This work is licensed under a Creative Commons Attribution 3.0 License.