Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (6): 336-340.doi: 10.12016/j.issn.2096-1456.2016.06.004

• Basic Study • Previous Articles     Next Articles

Influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma

Li-na YU1(),Wen-xuan ZHANG1,Yang YU2,Wen-guang QIN1,Xue-qi GUO1   

  1. 1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China
  • Received:2016-02-15 Revised:2016-03-25 Online:2016-06-20 Published:2016-06-20
  • Contact: Li-na YU E-mail:yulina2010@hotmail.com

Abstract:

Objective To investigate the expression of LASP-1 in tongue squamous cell carcinoma, and to study the influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma.Methods Green fluorescence shRNA targeting LASP-1 was constructed and transfected into SCC9 cells by lipofectamine. Cell proliferation was detected by MTT method. LASP-1 mRNA and protein were determined by RT-PCR and western blot respectively. The migration ability of cells was detected by transwell assay.Results There was green fluorescence expression in experimental and negative control group cells after transfecting shRNA and shNC respectively. LASP-1 mRNA and protein were decrease in experimental group cells. It indicated that LASP-1 shRNA was transfected successfully. By contrast with control group, the cell viability of experimental group cells reduced by (51.23 ± 1.47)% and (50.07 ± 2.11)% after 48 h and 72 h respectively. The results of transwell assay showed that migration ability of SCC9 cells was decreased significantly after shRNA targeting LASP-1 was successful transfected. It decreased by 43% compared with control group.Conclusion LASP-1 has high expression in tongue squamous cell carcinoma cells, down regulated LASP-1 gene expression can inhibit the proliferation and migration of SCC9 cells.

Key words: LASP-1, shRNA, Tongue squamous cell carcinoma, Cell proliferation, Migration

CLC Number: 

  • R739.86
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