Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (7): 390-394.doi: 10.12016/j.issn.2096-1456.2016.07.003

• Basic Study • Previous Articles     Next Articles

Effects of LMK-235 on osteoblast/odontoblast differentiation in hPDLCs

Qian-qian HAN1,Zhao LIU2,Li JIANG1,Hui-yi TANG1,Xiao-na LI3()   

  1. 1. Department of Periodontology, Guangdong Provincial Stomatological Hospital, the Affiated Stomstological Hospital of Southern Medical University, Guangzhou 510280, China
  • Received:2016-03-12 Revised:2016-04-07 Online:2016-07-20 Published:2016-07-20
  • Contact: Xiao-na LI E-mail:78lxn@163.com

Abstract:

Objective To investigate the effects of type Ⅱa histone deacetylase inhibitor LMK-235 during early osteoblast/odontoblast differentiation in hPDLCs.Methods hPDLCs were obtained by the collagenase digestion method. hPDLCs at the 3 rd passage were treated with medium containing 10% fetal bovine serum mixed with different concentrations of LMK-235 (0, 50, 100, 250, 500 nmol/L), respectively. Proliferative capability of hPDLCs was tested by MTT and qRT-PCR was used to detect mRNA expression levels of Runx2, ALP and DMP-1 3 d later. Results MTT assay showed that cell proliferation in hPDLCs treated with 100 nmol/L LMK-235 was increased significantly compared with the control group (P<0.05). The expression of Runx2 mRNA in the 100 nmol/L group was 1.77 times of the control groups (P<0.05). The expressions of ALP mRNA in all the experimental groups were significantly higher than that in control groups (P<0.05), and the expression in the 100 nmol/L groups was the highest. The expressions of DMP-1 mRNA in the 50 and 100 nmol/L groups were higher than the control groups (P<0.05). Conclusion Type Ⅱa histone deacetylase inhibitor LMK-235 could accelerate cell proliferation in hPDLCs at the concentration of 100 nmol/L, and regulate early osteoblast/odontoblast differentiation by upregulating the mRNA expressions of Runx2, ALP and DMP-1.

Key words: Periodontal ligament cells, Osteoblastic differentiation, Odontoblast differentiation, Histone deacetylase, Cell proliferation

CLC Number: 

  • R781.4

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引物名称 引物序列
GAPDH 上游5′-TGTGGGCATCAATGGATTTGG-3′
下游5′-ACACCATGTATTCCGGGTCAAT-3′
Runx2 上游5′-TGGTTACTGTCATGGCGGGTA-3′
下游5′-TCTCAGATCGTTGAACCTTGCTA-3′
ALP 上游5′-GGCCTGTACCATACAAGCCC-3′
上游5′-CCACGTAGACGAGGTAGTTGTG-3′
DMP-1 上游5′-CTCCGAGTTGGACGATGAGG-3′
下游5′-TCATGCCTGCACTGTTCATTC-3′

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