Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (8): 459-463.doi: 10.12016/j.issn.2096-1456.2016.08.005

• Basic Study • Previous Articles     Next Articles

Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells

Jun WEN,Shuai-mei XU,Ying LIU()   

  1. Department of Conservative Dentistry & Endodontics, Guangdong Provincial Stomatological & the Affiliated Stomatological Hospital of Southern Medical University, Guangzhou 510280, China
  • Received:2016-02-15 Revised:2016-03-24 Online:2016-08-20 Published:2016-08-20
  • Contact: Ying LIU E-mail:810906592@qq.com

Abstract:

Objective To evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and adipogenic differentiation of dental pulp stem cells (DPSCs).Methods The effect of XAV-939 on the proliferation of DPSCs was analyzed by cell counting kit-8(CCK-8). The effect of XAV-939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of glycogen synthase kinase-3β(GSK3β) and β-catenin which were Wnt signaling marker genes, and peroxisome proliferator activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) which were adipogenic marker genes.Results After 14 days, XAV-939 up-regulates expression of GSK3β、PPARγ2 and C/EBPα, while down-regulates β-catenin.Conclusion XAV-939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt signalling pathway.

Key words: Dental pulp stem cells, Adipogenic differentiation, Wnt passway, XAV-939, Cell proliferation

CLC Number: 

  • R781.3

Figure 1

The influence of XAV-939 with different cultural conditions on the proliferation of DPSCs"

Figure 2

The influence of XAV-939 on adipogenic differentiation of DPSCs"

Figure 3

Expression of GSK3β、PPARγ2、C/EBPα mRNA"

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