Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (9): 511-514.doi: 10.12016/j.issn.2096-1456.2016.09.003

• Basic Study • Previous Articles     Next Articles

Study on the role of long non-coding RNA TUG1 in tongue squamous cell carcinoma

LI Zhi-qiang1,ZOU Rui2,OUYANG Ke-xiong2,BAI Zhi-bao3,AI Wei-jian1()   

  1. 1. Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital, the Affiliated Stomatological Hospital of Southern Medical University, Guangzhou 510280, China
    2. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University; Guangzhou 510140, China
    3. Department of Stomatology Guangzhou First Peoples′ Hospital, Guangzhou 510180, China;
  • Received:2016-01-24 Revised:2016-02-05 Online:2016-09-20 Published:2016-09-20
  • Contact: Wei-jian AI E-mail:weijianai@139.com

Abstract:

Objective To study the expression of LncRNA TUG1 in tongue squamous cell carcinoma (TSCC), and explore its function in the development of TSCC.Methods Fresh samples of TSCC tissues and corresponding adjacent normal tongue tissues were obtained from 19 cases, and the expressions of TUG1were detected by Q-PCR. The expression of TUG1 in CAL27 cell line were down-regulated by siRNA technology, and the cell proliferation ability after transfection was analyzed by MTT assay. The expressions of iNOS gene were rated by Q-PCR.Results Result of Q-PCR revealed the expressions of LncRNA TUG1 were up-regulated in TSCC tissues compared with the adjacent normal tongue tissues (P < 0.001). Cell proliferation ability was inhibited after down-regulation of TUG1 by siRNA technology, and the inhibition rates of 24/48/72 hour were 14.16%. 16.96% and 21.40% respectively. The relative expression of iNOS in blank/control/experimental groups were 1.000 ± 0.034, 0.974 ± 0.045 and 0.729 ± 0.039 respectively, and iNOS was down- regulated significantly in the experimental group (P = 0.002).Conclusion LncRNA TUG1 may have functions as a cancerogenic substance in TSCC via pormoting the expression of iNOS.

Key words: Tongue, Squamous cell carcinoma, Long non-coding RNA, Taurine upregulated gene 1, Inducible nitric oxide synthase

CLC Number: 

  • R739.8

Table 1

Three alternative small interfering RNA sequences"

小干扰RNA 正义序列(5′-3′) 反义序列(5′-3′)
siRNA-1 GGCCUACCAUGUAGAGAAUTT AUUCUCUACAUGGUAGGCCTT
siRNA-2 GGCCUCUAUUCCUGUAUGUTT ACAUACAGGAAUAGAGGCCTT
siRNA-3 CCUCAGAUCUCAUCUAAAUTT AUUUAGAUGAGAUCUGAGGTT

Table 2

The optical density of MTT in 3 groups at different transfection times"

转染时间 空白对照组 阴性对照组 实验组
转染24 h 0.219 0.217 0.188
转染48 h 0.212 0.211 0.176
转染72 h 0.207 0.206 0.163

Table 3

The inshibitory rate of cell proliferation in the experimental group and negative control group at different transfection times"

转染时间 阴性对照组 实验组 χ2 P
转染24 h 0.59 14.16 11.40 0.001
转染48 h 0.63 16.96 8.72 0.002
转染72 h 0.48 21.40 15.87 < 0.001
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