Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (9): 519-523.doi: 10.12016/j.issn.2096-1456.2016.09.005

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Comparison of the bacterial community structures in root canals with different severity of infection by metagenomic analysis

ZHOU Qi(),LI Jun-xin,ZHONG Bo,ZHANG Xiao-jie,JIANG Shuang-feng   

  1. Department of Stomatology, Shenzhen No.2 People’s Hospital, Shenzhen 518035, China
  • Received:2016-05-17 Revised:2016-06-07 Online:2016-09-20 Published:2016-09-20
  • Contact: Qi ZHOU E-mail:kqk2012@sina.cn

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Abstract:

Objective To compare the intraradicular bacterial community structures in under-filling teeth with or withoutperiapical infection.Methods The intraradicularbiofilm samples were collected from under-filling teeth with periapicalinfection (apical periodontitis group, 3 teeth) and underfilling teeth withoutperiapical infection (non-apical periodontitis group, 2 teeth). The V3, V6 hypervariable regions of bacterial 16S rRNA genes were amplified, and the high-throughput sequencingwasperformed. Thecomposition and structure characteristic of intraradicular microbiota were revealed by bioinformatics analysis.Results The microbiotain apical periodontitis group comprised of Firmicutes, Proteobacteria, Actinobacteriad and others, total 22 phyla. While the microbiotain non-apical periodontitis group comprised of Firmicutes, Proteobacteria, Actinobacteria and others, total 8 phyla. The complexity of the microbiotain apical periodontitis groupwas significantly higher than without apical periodontitis group, but both groups comprised thesimilar most representivephyla.Conclusion The diversity of intraradicular bacterial community in underfillingteeth with apical periodontitis was higher than those in non-apical periodontitis.

Key words: Periapical periodontitis, Metagenomic analysis, Biofilm, Microbiota, Diversity

CLC Number: 

  • R781.34

Figure 1

The testing result of 16S V3 region"

Figure 2

The testing result of 16S V6 region"

Table 1

The microbiotain apical periodontitis group and non-apical periodontitis group"

#OTU ID PC318 PC311 PC315 PC313 PC314
p__Proteobacteria变形菌门 0.5088729 0.3156581 0.1606141 0.0465165 0.0814483
p__Firmicutes厚壁菌门 0.4204039 0.5028336 0.2933948 0.0986398 0.1394935
Unassigned;Other 0.0653958 0.1788531 0.543669 0.8540131 0.7779912
p__Actinobacteria放线菌门 0.0019491 0.0005548 0.0006773 0.0001557 0.0001423
Other 0.0013551 0.0015852 0.0008063 0.0003634 0.0004268
p__Bacteroidetes拟杆菌门 0.0008353 7.93E-05 0.000129 0.0001557 0.0001423
p__Chloroflexi弯绿菌门 0.0002599 0.0001585 0.0002258 0.0001557 7.11E-05
p__Planctomycetes浮霉菌门 0.0002599 0 0.000258 0 0
p__[Thermi] 0.0001671 0.0001585 0 0 7.11E-05
p__Acidobacteria酸杆菌门 9.28E-05 3.96E-05 0 0 0
p__Cyanobacteria蓝藻菌门 9.28E-05 3.96E-05 0 0 0
p__Fusobacteria梭杆菌门 9.28E-05 0 0 0 0
p__Synergistetes互养菌门 7.43E-05 0 0 0 7.11E-05
p__AD3 1.86E-05 3.96E-05 0 0 0.0001423
p__GN01 1.86E-05 0 0 0 0
p__GN02 1.86E-05 0 0 0 0
p__Gemmatimonadetes 1.86E-05 0 0 0 0
p__SAR406 1.86E-05 0 0 0 0
p__TM7 1.86E-05 0 3.23E-05 0 0
p__Verrucomicrobia 1.86E-05 0 9.68E-05 0 0
p__WPS-2 1.86E-05 0 0 0 0
p__Chlorobi 0 0 3.23E-05 0 0
p__Spirochaetes 0 0 3.23E-05 0 0
p__Tenericutes 0 0 3.23E-05 0 0

Figure 3

The comparison of dominant-phyla"

Figure 4

The comparison of dominant-microbiota"

[1] Sakamoto M, Siqueira JF Jr , et al. Molecular analysis of the root canal microbiota associated with endodontic treatment failures[J]. Oral Microbiol Immunol, 2008,23(4):275-281.
doi: 10.1111/j.1399-302X.2007.00423.x
[2] Gomes BP, Pinheiro ET, Gade-Neto CR , et al. Microbiological examination ofinfected dental root canals[J]. Oral Microbiol Immunol, 2004,19(2):71-76.
doi: 10.1046/j.0902-0055.2003.00116.x
[3] Sunde PT, Olsen I, Debelian GJ , et al. Microbiota of periapical lesions refractory to endodontic therapy[J]. J Endod, 2002,28(4):304-310.
doi: 10.1097/00004770-200204000-00011
[4] Lana MA, Ribeiro-Sobrinho AP , Stehling R, etal. Microorganisms isolated from rootcanals presenting necrotic pulp and their drug susceptibility in vitro[J]. Oral Microbiol Immunol, 2001,16(2):100-105.
doi: 10.1034/j.1399-302x.2001.016002100.x
[5] Aas JA, Paster BJ, Stokes LN , et al. Definingthe normal bacterialflora of the oral cavity[J]. J Clin Microbiol, 2005,43(11):5721-5732.
doi: 10.1128/JCM.43.11.5721-5732.2005
[6] Faveri M, Mayer MP, Feres M , et al. Microbiological diversity of generalizedaggressive periodontitis by 16S rRNA clonal analysis[J]. Oral Microbiol Immunol, 2008,23(2):112-118.
doi: 10.1111/omi.2008.23.issue-2
[7] Sakamoto M, Rocas IN, Siqueira JF , et al. Molecular analysis of bacteria inasymptomatic and symptomaticendodontic infections[J]. Oral Microbiol Immunol, 2006,21(2):112-122.
doi: 10.1111/omi.2006.21.issue-2
[8] Moiler AJ . Microbiological examination of root canals andperiapical tissues of human teeth. Methodological studies[M]. Odontol Tidskr, 1966,74(5 Suppl):1-380.
[9] Rappe MS, Giovacmoni SJ . The uncultured microbial majority[J]. Annu Rev Microbiol, 2003,57(1):369-394
doi: 10.1146/annurev.micro.57.030502.090759
[10] He XS, Shi WY . Oral microbiology: past, present and future[J]. Int J Oral Sci, 2009,1(2):47-58.
doi: 10.4248/ijos.09029
[11] Yang F, Zeng X, Ning K , et a1. Salivamicrobiomes distinguishcaries-active from healthy human populations[J]. ISME J, 2012,6(1):1-10.
[12] Colombo AP, Boces SK , et al. Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis and periodontal health using the human oral microbe identification microarray[J]. Periodontol, 2009,80(9):1421-1432.
doi: 10.1902/jop.2009.090185
[13] 王娟, 唐志娟, 李谨 , 等. 根管治疗后伴或不伴根尖周炎患牙根管内微生物群落的比较分析[J]. 中华口腔医学杂志, 2014,49(10):607-613.
[14] Adriana C, Ribeiro , et al. Exploring bacterial diversity of endodontic microbiotaby cloning and sequencing 16S rRNA[J]. J Endod, 2011,37(7):922-926.
doi: 10.1016/j.joen.2011.04.007
[15] Munson MA, Pitt-Ford T, Chong B , et al. Molecular and culturalanalysis of the micrflora associated with endodontic infections[J]. J Dent Res, 2002,81(11):761-766.
doi: 10.1177/0810761
[16] Sakamoto M, Siqueira JF, Rocas IN , et al. Bacterial reduction and persistenceafter endodontic treatmentprocedures[J]. Oral Microbiol Immunol, 2007,22(1):19-23.
doi: 10.1111/omi.2007.22.issue-1
[17] 全鹤, 王聃, 季晓妮 . 光动疗法辅助根管消毒治疗慢性根尖周炎的临床效果[J]. 广东牙病防治, 2015,23(12):640-642.
[18] 凌均棨, 韦曦, 刘红艳 . 难治性根尖周炎的病因及防治策略[J]. 中华口腔医学杂志, 2010,45(1):52-57.
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