Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (10): 578-581.DOI: 10.12016/j.issn.2096-1456.2016.10.004

• Basic Study • Previous Articles     Next Articles

An in vitro evaluation of antibacterial properties of 2 pulp capping materials

Hai-xia LIU,Xuan CHEN,Ling ZOU()   

  1. State Key Laboratory of Oral Diseases, Department of Conservative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2016-04-13 Revised:2016-05-15 Online:2016-10-20 Published:2016-10-20
  • Contact: Ling ZOU

两种盖髓材料的体外抗菌性能研究

刘海霞,陈璇,邹玲()   

  1. 口腔疾病研究国家重点实验室,四川大学华西口腔医院牙体牙髓病科,四川 成都(610041)
  • 通讯作者: 邹玲
  • 作者简介:刘海霞,医师,硕士, Email: lhx1245390385@163.com
  • 基金资助:
    四川省科技计划项目(2015JY0260)

Abstract:

Objective To evaluate the antimicrobial properties of 2 different pulp capping materials (ProRoot MTA and iRoot BP Plus).Methods Modified direct contact test (DCT) was performed as follow. ProRoot MTA and iRoot BP Plus were set for 7 days, then crushed and immersed into deionized water at concentrations of 200 g·L -1 for another 7 days. PH values of the supernatants of the aqueous extracts were measured with a pH meter. The germfree supernatants were mixed with Streptococcus mutans suspensions and then incubated for 24 hours. The survival of bacteria was assessed by measuring the optical density (OD) at 600 nm with an automated microplate reader.Results PH values of the aqueous extracts of ProRoot MTA and iRoot BP Plus were between 12 and 13, and no statistically significant difference was found (t = 0.133, P = 0.899). Both ProRoot MTA and iRoot BP Plus demonstrated good antibacterial properties (P < 0.05), and iRoot BP Plus performed a better antibacterial effect (P < 0.05).Conclusion IRoot BP Plus performed a better antibacterial effect against Streptococcus mutans when compared to ProRoot MTA.

Key words: Antibacterial property, ProRoot MTA, iRoot BP Plus, Streptococcus mutans, Direct contact test

摘要:

目的 探讨ProRoot MTA和iRoot BP Plus 2 种盖髓材料的体外抗菌性能。方法 采用改良直接接触实验(direct contact test,DCT),将新鲜配制的ProRoot MTA和iRoot BP Plus固化7 d后研磨成粉末,分别以200 g·L -1质量体积比浸泡于去离子水中7d,用pH计测量各材料浸提液上清pH值。取浸提液无菌上清与变异链球菌的菌悬液分别混合培养24 h,酶标仪测量各组混合液600 nm的吸光度(optical density,OD)。结果 2种材料浸提液上清pH值均介于12~13之间,差异无统计学意义(t = 0.133,P = 0.899);2种材料均表现出对变异链球菌的抑制作用(P<0.05),且iRoot BP Plus的抑菌作用强于ProRoot MTA(P<0.05)。结论 iRoot BP Plus对变异链球菌的抑制作用强于ProRoot MTA。

关键词: 抗菌性能, ProRoot MTA, iRoot BP Plus, 变异链球菌, 直接接触实验

CLC Number: