Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (11): 634-639.doi: 10.12016/j.issn.2096-1456.2016.11.003

• Basic Study • Previous Articles     Next Articles

Effects of osteoprotegerin on implant osseointegration in ovariectomized rats

LIU Xian-wen1,ZHANG Chao2,LIU Shu-guang1,AI Wei-jian1,ZHOU Hui-xi1,LI Yun-fen3()   

  1. 1. Department of Oral and Maxillofacial Surgery, Guangdong Provincial Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China
    2. Department of Orthodontics, Guangdong Provincial Hospital of Stomatology, Southern Medical University, Guangzhou 510280, China
    3. Center of Orthognathic and Temporomandibular Joint Surgery, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China
  • Received:2016-08-03 Revised:2016-09-07 Online:2016-11-20 Published:2016-11-20
  • Contact: Yun-fen LI E-mail:li-yun-fen@gmail.com

Abstract:

Objective To investigate the role of osteoprotegerin (OPG) in implant osseointegration in ovariectomized rats.Methods 12 weeks after bilateral ovariectomy, each rat accepted 2 titanium screws in the proximal tibiae. All animals were then randomly divided into 2 groups: control (10 rats) and OPG group (10 rats). Subcutaneous injection of OPG (10 mg/kg) or vehicle was performed 3 times a week. 8 weeks later, tibiae with screws were harvested for μCT, histological and biomechanical analysis.Results Compared to control, OPG increased the percent bone volume by 124%, the percent osseointegration by 167%, the mean trabecular number by 111%, the mean trabecular thickness by 92%, the mean connective density by 95%; and decreased the mean trabecular separation by 64% in μCT analysis. OPG also increased bone area density by 160% and bone-to-implant contact by 234% in histomorphometric evaluation, and increased the maximal push-out force by 228% in biomechanical test.Conclusion Systemic administration of OPG can improve implant osseointegration and fixation in ovariectomized rats, resulting from the increased peri-implant bone mass and improved trabecular microarchitecture.

Key words: Implant, Osseointegration, Osteoporosis, Osteoprotegerin, Rat

CLC Number: 

  • R783.1

Figure 1

Surface morphology of implant by scanning electron microscopy"

Figure 1

μCT analysis"

Figure 3

μCT cross sectional images and quantitative analysis of bone mass around implants and implant bone integration"

Figure 4

1% toluidine blue staining of transverse sections in OPG group and control group"

Figure 5

Histological evaluation and biomechanical test"

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