Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (1): 31-37.doi: 10.12016/j.issn.2096-1456.2018.01.006

• Basic Study • Previous Articles     Next Articles

Investigation of the expression of TPX2 in tongue squamous cell carcinoma and its effect on Cal27 cell proliferation and apoptosis

Songhao YIN1(), Weihong GUO2   

  1. 1. Department of Oral and Maxillofacial Surgery, People's Hospital, Dongguan 523000, China;
    2. Department of First Medical College, Southern Medical University, Guangzhou 510515, China
  • Received:2017-05-16 Revised:2017-07-18 Published:2018-08-31

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Abstract:

Objective To investigate the expression of targeting protein for xenopus kinesin-like protein2 (TPX2) in tongue squamous cell carcinoma (TSCC) tissues and explore its effect on cell proliferation and apoptosis. Methods 30 cases of TSCC tissues, paired normal tissues were collected in Dongguan People's Hospital during 2013-2016. The mRNA and protein expression level of TPX2 was determined by qRT-PCR and western blot, respectively and analyzed The correlation of TPX2 expression level and clinic opathological parameters. Cal27 cell was transfected with siRNAs to knockdown the expression of TPX2, then cell proliferation, cell apoptosis and related proteins (cleaved caspase 3 and caspase 3) were detected by MTT assay, flow cytometry and western blot. Results TPX2 was highly expressed in (t=3.254, P=0.002 9) tumor tissues at mRNA level compared to adjacent normal parts. In protein level, TPX2 was highly expressed (66.7%) in tumor tissues, TPX2 expression level of 20 case was higher than the corresponding tissue adjacent to carcinoma (20/30, t=2.862, P=2.862), and high expression of TPX2 was related to T staging, lymph node metastasis of tongue cancer. Knockdown TPX2 effectively reduced cell proliferation, increased apoptosis rate (F=342.9, P < 0.000 1) and upregulated the expression of apoptosis-related proteins cleaved caspase 3 (F=46.98, P=0.001 4) and caspase 3 (F=33.35, P=0.002 7). Conclusion Overexpression of TPX2 was found in TSCC tissues. Silencing of TPX2 might inhibit cell proliferation and promote cell apoptosis. TPX2 could be a new target for gene therapy of TSCC.

Key words: TSCC, TPX2, Cell proliferation, Apoptosis, Cal27 cell

CLC Number: 

  • R739.8

Figure 1

Expression of TPX2 mRNA and protein in TSCC tissues and adjacent normal tissues"

Figure 2

Effect of TPX2 siRNA the expression of TPX2 mRNA and protein"

Figure 3

Effect of TPX2 on proliferation of Cal27 cells"

Figure 4

Effect of TPX2 on apoptosis of Cal27 cells"

Table 1

Correlation between TPX2 expression and clinicopathological parameters of patients with TSCC n"

病理参数 n TPX2mRNA高表达 χ2值 P 值 TPX2蛋白高表达 χ2值 P 值
性别 男 18 14 0.031 0.860 12 0.000 1.000
女 12 9 8
年龄 <60岁 14 10 0.403 0.526 10 0.268 0.605
≥60岁 16 13 10
组织分化 高中分化 22 17 0.017 0.896 13 2.131 0.144
低分化 8 6 7
T分期 T1,T2 13 7 6.679 0.010 6 4.344 0.037
T3,T4 17 16 14
淋巴结转移 有 16 14 5.000 0.025 15 11.317 0.001
无 14 7 5

Figure 5

Effect of TPX2 on apoptos related proteins in Cal27 cells"

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