Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (4): 227-230.doi: 10.12016/j.issn.2096-1456.2018.04.005

• Basic Study • Previous Articles     Next Articles

Expression of hypoxia-inducible factor 1α in the pressure-side periodontium during orthodontic tooth movement in rats

Zhengquan HE1(), Kai YANG2()   

  1. 1. Department of Orthodontics,Changsha Stomatological Hospital, Changsha 410004, China
    2. Department of Orthodontics, Beijing Stomatological Hospital, Beijing 100050, China
  • Received:2017-07-18 Revised:2017-12-09 Online:2018-04-20 Published:2018-08-31

Abstract:

Objective To assess hypoxia-inducible factor 1α (HIF-1α) expression levels in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.Methods A total of 50 male 6-week-old Sprague Dawley (SD) rats were randomly divided into 10 groups of 5 rats each. The upper left first molar was the experimental tooth and was pulled mesially with an orthodontic force of 10-15 g for 0, 1, 3, 6, or 12 h, or 1, 3, 7, 14, or 21 d. Routine five-micrometer paraffin-embedded tissue sections were processed for HE staining and immunohistochemical staining for HIF-1α. The Image-Pro Plus system was used to quantitatively analyze the stained slices. The expression of HIF-1α in the periodontal tissue of the pressure side changed during the process of orthodontic tooth movement.Results The expression of HIF-1α increased immediately after loading for 1 h, reached a small peak at 3 h, and then decreased. After 12 h, the expression increased again, reached a peak after 1 d, and then gradually decreased to near the pre-loading level(P < 0.05). Conclusion There were differences in expression of HIF-1α in different groups in the periodontal tissues of the pressure side during orthodontic tooth movement in rats.

Key words: Orthodontics, Tooth movement, Periodontal tissue remodeling, Hypoxia, Inducible factor 1α

Figure 1

Model of orthodontic tooth movement in rats"

Figure 2

HE staining in the control group"

Figure 3

Expression of HIF-1α by immunohistochemical staining in the different groups (× 100)"

Figure 4

Changes in HIF-1α average optical density over time in the orthodontic tooth movement pressure region in rats"

Table 1

Comparison of HIF-1 alpha average optical density in different time groups of orthodontic forces in rats \(\overline{x}\)± s"

组别 压力区
0 h 0.0790±0.0081
1 h 0.0886±0.01141)
3 h 0.1034±0.01071)2)
6 h 0.0919±0.00951) 2)
12 h 0.0956±0.01061) 2)
1 d 0.1211±0.01371) 2)
3 d 0.1177±0.01101) 2)
7 d 0.1051±0.01191) 2)
14 d 0.0941±0.00891) 2)
21 d 0.0876±0.00961) 2)
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