Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (7): 434-439.doi: 10.12016/j.issn.2096-1456.2018.07.005

• Basic Study • Previous Articles     Next Articles

Influence of luteolin on the invasion and migration of an human tongue squamous carcinoma cell line

Xiaojin WANG1(), Xinchao YOU2, Kai CHEN1, Kunsong HUANG1, Xuan PAN1()   

  1. 1. Department of Stomatology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China
    2. Central Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China
  • Received:2018-04-24 Revised:2018-05-02 Online:2018-07-20 Published:2018-08-30

Abstract:

Objective To investigate the effects of luteolin on the invasion and migration of the human tongue squamous carcinoma cell line SCCl5. Methods SCC15 cells were treated with various concentrations of luteolin (5, 10, 15, 20, 40 and 60 μg/mL) for 24, 48 and 72 h. The MTT assay was then carried out to estimate the proliferation of SCC15 cells treated with various concentrations of luteolin. SCC15 cells were treated with various concentrations of luteolin (1, 5 and 10 μg/mL), and the migration of SCC15 cells was examined in wound healing assays. SCC15 cells were treated with various concentrations of luteolin (5 and 10 μg/mL) for 24 h, and the migration and invasion of the cells were examined in Transwell migration/invasion assays. SCC15 cells were treated with various concentrations of luteolin (10, 20 and 40 μg/mL) for 24 h, and the conditioned medium was collected. The levels of the gelatinases matrix metalloproteinases-2 and -9 (MMP-2, MMP-9) in the conditional medium were detected by gelatin zymography assays. Results The MTT assay showed that luteolin had a substantial inhibitory effect on the proliferation of SCC15 cells in a concentration- and time-dependent manner (P < 0.01). The migration, invasion and proliferation of the SCCl5 cell lines were significantly lower after treatment with luteolin than in the control. The numbers of migrating and invading SCCl5 cells were 340.00 ± 22.94, 52.67 ± 6.94 and 6.57 ± 0.80 versus 85.67 ± 5.18, 39.67 ± 4.63 and 2.67 ± 0.29, respectively (P < 0.01). The enzyme activities of MMP-2 and MMP-9 decreased significantly in response to luteolin treatment in a concentration-dependent manner (P < 0.01). Conclusion Luteolin inhibited the invasion and migration of SCC15 cells by reducing the activities of MMP-2 and MMP-9.

Key words: Luteolin, Oral Carcinoma, Squamous cell, Cell proliferation, Cell invasion, Cell migration

CLC Number: 

  • R738.9

Figure 1

Inhibitory effect of luteolin on the proliferation of SCC15 cells"

Figure 2

Effect of luteolin treatment on the cell migration ability of SCC15 cells"

Figure 3

Luteolin inhibits the migration and invasion of SCC15 cells"

Figure 4

Zymogen expression of MMP-2 and MMP-9 in SCC15 cells treated with luteolin for 24 h"

[1] Ferlay J, Soerjomataram I, Dikshit R, et al.Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012[J]. Int J Cancer, 2015, 136(5): E359-E386.
[2] Chen P, Zhang JY, Sha BB, et al.Luteolin inhibits cell proliferation and induces cell apoptosis via down-regulation of mitochondrial membrane potential in esophageal carcinoma cells EC1 and KYSE450[J]. Oncotarget, 2017, 8(16): 27471-27480.
[3] Kwon K, Kwon YS, Kim SW, et al.Luteolin-induced apoptosis through activation of endoplasmic reticulum stress sensors in pheochromocytoma cells[J]. Mol Med Rep, 2017,16(1): 380-386.
[4] Cao Z, Zhang H, Cai X, et al.Luteolin promotes cell apoptosis by inducing autophagy in hepatocellular carcinoma[J].Cell Physiol Biochem, 2017, 43(5): 1803-1812.
[5] Lu X, Li Y, Li X, et al.Luteolin induces apoptosis in vitro through suppressing the MAPK and PI3K signaling pathways in gastric cancer[J]. Oncol Lett, 2017,14(2): 1993-2000.
[6] Lin D, Kuang G, Wan J, et al.Luteolin suppresses the metastasis of triple-negative breast cancer by reversing epithelial-to-mesenchymal transition via downregulation of β-catenin expression[J].Oncol Rep, 2017, 37(2): 895-902.
[7] Parka SH, Parkb HS, Leec JH, et al.Induction of endoplasmic reticulum stress-mediated apoptosis and non-canonical autophagy by luteolin in NCI-H460 lung carcinoma cells[J]. Food Chem Toxicol, 2013, 56: 100-109.
[8] Huang X, Dai S, Dai J, et al.Luteolin decreases invasiveness, deactivates STAT3 signaling, and reverses interleukin-6 induced epithelial-mesenchymal transition and matrix metalloproteinase secretion of pancreatic cancer cells[J]. Onco Targets Ther, 2015, 8: 2989-3001.
[9] Wang S, Liu Q, Zhang YS, et al.Suppression of growth, migrationand invasion of highly-metastatic human breast cancer cells byberbamine and its molecular mechanisms of action[J]. Mol Cancer, 2009, 8: 81-89.
[10] Maluccio M, Covey A.Recent progress in understanding, diagnosing, and treating liepatocellular carcinoma[J]. CA Cancer J Clin, 2012, 62(6): 394-399.
[11] Lee JC, Chung LC, Chen YJ, et al.N-myc downstream-regulated gene 1 downregulates cell proliferation, invasiveness, and tumorigenesis in human oral squamous cell carcinoma[J].Cancer Lett, 2014, 355(2): 242-252.
[12] Fan H, Li H, Liu G, et al.Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts[J]. J Exp Clin Cancer Res, 2017, 36(1): 163-175.
[13] Zhang CX, Ye LW, Liu Y, et al.Antineoplastic activity of Newcastle disease virus strain D90 in oral squamous cell carcinoma[J]. Tumor Biol, 2015, 36(9): 7121-7131.
[14] Wang Q, Wang H, Jia Y, et al.Luteolin reduces migration of human glioblastoma cell lines via inhibition of the p-IGF-1R/PI3K/AKT/mTOR signaling pathway[J]. Oncol Lett, 2017, 14(3): 3545-3551.
[15] Wang L, Ling Y, Chen Y, et al.Flavonoid baicalein suppresses adhesion, migration and invasion of MDA-MB-231 human breast cancer cells[J].Cancer Lett, 2010, 297(1): 42-48.
[16] Huang Q, Lan F, Wang X, et al.IL-1β-induced activation of p38 promotes metastasis in gastric adenocarcinoma via upregulation of AP-1/c-fos, MMP2 and MMP9[J]. Mol Cancer, 2014, 13(1): 18-32.
[17] Chen CM, Hsieh SC, Lin CL, et al.Alpha-Mangostin suppresses the metastasis of human renal carcinoma cells by targeting mek/erk expression and mmp-9 transcription activity[J]. Cell Physiol Biochem, 2017, 44(4): 1460-1470.
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