Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (10): 627-633.doi: 10.12016/j.issn.2096-1456.2018.10.003

• Basic Study • Previous Articles     Next Articles

Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis

Hongling LIANG(),Bin CHEN,Shasha HE,Xiaoting XIE,Fuhua YAN()   

  1. Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
  • Received:2018-06-07 Revised:2018-07-01 Online:2018-10-20 Published:2018-10-20

Abstract:

Objective To investigate the distribution and proportion of M1/M2 macrophages in the periodontal tissues of rats with and without periodontitis.Method Twelve Sprague-Dawley rats were randomly divided into a chronic periodontitis group (CP, n = 6) and a periodontal health group (PH, n = 6). The periodontitis model was induced at the first mandibular molar using a stainless steel ligature and was confirmed by histological analysis. M1 macrophages were labeled with inducible nitric oxide synthase (iNOS), and M2 macrophages were labeled with CD163. The distributions of M1 and M2 macrophages in the two groups were determined via immunohistochemistry and immunofluorescence, and the M1/M2 ratios were compared between the two groups.Results The M1 type macrophage count in the PH group was 12.17 ± 1.40, and the M1 macrophage count in the CP group was 40.00 ± 3.20; there was a statistically significant difference between the two groups (t = 7.96, P<0.0001). The M2 macrophage count in the PH group was 4.50 ± 1.09, and the M2 type macrophage count in the CP group was 5.33 ± 0.67. There was no statistically significant difference between the two groups (t = 0.65, P = 0.53). The M1/M2 ratio in the CP group was 3.72 ± 1.08, and the M1/M2 ratio in the PH group was 8.31 ± 1.37; there was a statistically significant difference between the two groups (t = 2.63, P= 0.025).Conclusion During periodontitis, M1 macrophages increased significantly and were widely distributed; they may be involved in the progression of periodontitis and may be closely related to the destruction of the cementum.

Key words: Periodontitis, Periodontal tissue, Periodontitis model, Macrophages, M1 macrophages, M2 macrophages

CLC Number: 

  • R781.4

Figure 1

HE staining of PH and CP groups"

Figure 2

Immunohistochemical staining for iNOS in M1 macrophages in the PH and CP groups"

Figure 3

Immunohistochemical staining for CD163 in M2 macrophage in the PH and CP groups"

Figure 4

Immunohistochemical staining for iNOS in M1 macrophages in the PH and CP groups × 200"

Figure 5

Immunohistochemical staining for CD163 in M2 macrophages in the PH and CP groups × 200"

[1] Schenkein HA . Host responses in maintaining periodontal health and determining periodontal disease[J]. Periodontol 2000, 2006,40:77-93.
doi: 10.1111/j.1600-0757.2005.00144.x pmid: 16398686
[2] Cui D, Lyu J, Li HX , et al. Human beta-defensin 3 inhibits periodontitis development by suppressing inflammatory responses in macrophages[J]. Mol Immunol, 2017,91:65-74.
[3] Nagashima H, Shinoda M, Honda K , et al. CXCR4 signaling in macrophages contributes to periodontal mechanical hypersensitivity in Porphyromonas gingivalis-induced periodontitis in mice[J]. Mol Pain, 2017,13:1744806916689269.
[4] Gordon S, Martinez FO . Alternative activation of macrophages: mechanism and functions[J]. Immunity, 2010,32(5):593-604.
doi: 10.1016/j.immuni.2010.05.007
[5] Das A, Sinha M, Datta S , et al. Monocyte and macrophage plasticity in tissue repair and regeneration[J]. Am J Pathol, 2015,185(10):2596-2606.
doi: 10.1016/j.ajpath.2015.06.001 pmid: 26118749
[6] Mylonas KJ, Jenkins SJ, Castellan RF , et al. The adult murine heart has a sparse, phagocytically active macrophage population that expands through monocyte recruitment and adopts an M2 phenotype in response to Th2 immunologic challenge[J]. Immunobiology, 2015,220(7):924-933.
[7] Martinez FO, Helming L, Gordon S . Alternative activation of macrophages: an immunologic functional perspective[J]. Annu Rev Immunol, 2009,27:451-483.
doi: 10.1146/annurev.immunol.021908.132532 pmid: 19105661
[8] Murray PJ, Wynn TA . Obstacles and opportunities for understanding macrophage polarization[J]. J Leukoc Biol, 2011,89(4):557-563.
doi: 10.1189/jlb.0710409 pmid: 21248152
[9] Wilson HM . SOCS proteins in macrophage polarization and function[J]. Front Immunol, 2014,5:357.
doi: 10.3389/fimmu.2014.00357 pmid: 4112788
[10] Bashir S, Sharma Y, Elahi A , et al. Macrophage polarization: the Link between inflammation and related diseases[J]. Inflamm Res, 2016,65(1):1-11.
doi: 10.1007/s00011-015-0874-1 pmid: 26467935
[11] Gemmell E, Mchugh GB, Grieco DA , et al. Costimulatory molecules in human periodontal disease tissues[J]. J Periodontal Res, 2001,36(2):92-100.
doi: 10.1034/j.1600-0765.2001.360205.x pmid: 11327084
[12] 冯利, 冯洁, 王春华 , 等. 牙周炎龈组织中巨噬细胞超微结构的观察[J]. 四川大学学报:自然科学版, 2002 ( S1):90-92.
[13] Chang MK, Raggatt LJ, Alexander KA , et al. Osteal tissue macrophages are intercalated throughout human and mouse bone lining tissues and regulate osteoblast function in vitro and in vivo[J]. J Immunol, 2008,181(2):1232-1244.
[14] Alexander KA, Chang MK, Maylin ER , et al. Osteal macrophages promote in vivo intramembranous bone healing in a mouse tibial injury model[J]. J Bone Miner Res, 2011,26(7):1517-1532.
doi: 10.1002/jbmr.354 pmid: 21305607
[15] Gordon S, Taylor PR . Monocyte and macrophage heterogeneity[J]. Nat Rev Immunol, 2005,5(12):953-964.
[16] Gonzalez OA, Novak MJ, Kirakodu S , et al. Differential gene expression profiles reflecting macrophage polarization in aging and periodontitis gingival tissues[J]. Immunol Invest, 2015,44(7):643-664.
[17] Yu T, Zhao L, Huang X , et al. Enhanced activity of the macrophage M1/M2 phenotypes and phenotypic switch to M1 in periodontal infection[J]. J Periodontol, 2016,87(9):1092-1102.
[18] Lam RS, O′brien-Simpson NM, Lenzo JC , et al. Macrophage depletion abates Porphyromonas gingivalis-induced alveolar bone resorption in mice[J]. J Immunol, 2014,193(5):2349-2362.
doi: 10.4049/jimmunol.1400853 pmid: 25070844
[19] Gordon S . Alternative activation of macrophages[J]. Nat Rev Immunol, 2003,3(1):23-35.
[20] Martinez FO, Sica A, Mantovani A , et al. Macrophage activation and polarization[J]. Front Biosci, 2008,13:453-461.
[1] WU Hongyu,MA Xiaoxin,LU Haixia,FENG Xiping,GU Qin,YE Wei,XIE Yingxin,XIE Danshu,WANG Wenji. Investigation of dental caries and periodontal conditions in maintenance hemodialysis patients [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 313-317.
[2] ZHANG Limu,LIN Xiaoping. Research progress on the mechanism of C-reactive protein mediated periodontitis and systemic diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 184-188.
[3] WEI Shimin,WANG Yuanjing,HUANG Wen,CHEN Yifan,YANG Renli,QU Yili. Research progress in the regulation of macrophages in foreign body reaction in bone tissue repair [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(9): 591-597.
[4] LI Jiatong,ZHOU Xuedong,XU Xin,WANG Yan. Research progress of probiotics in the prevention and treatment of oral infectious diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(9): 598-601.
[5] Gang WANG,Peng SUN,Juan LI,Shiguang HUANG. Expression of the receptor of advanced glycation end products in gingival tissue endothelial cells from type 2 diabetic rats with chronic periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 428-434.
[6] DAI Qian,YU Yuanling,HUANG Shiguang. Macrophage IL-33 expression in macrophages of human chronic periapical periodontitis and apical cyst [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(5): 300-303.
[7] XU Xiongcheng,ZHONG Quan,LUO Kai. Application of 3D printing technology in preparation of scaffolds for periodontal tissue regeneration [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 189-193.
[8] ZHU Yanxia,Gegen tana. Research progress of IL-33/ST2 signaling pathway in bone metabolism [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 202-204.
[9] WANG Ye,LIN Xiaoping. Research progress on the common risk factors and related mechanisms of periodontitis and osteoporosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 794-798.
[10] WANG Min, GE Song. Research progress on the T cell receptor CDR3 spectral sequence of periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 799-803.
[11] MA Yao,JIANG Zhaowei,JIN Yunyi,MIAO Qian,ZHANG Chunxiang,ZHANG Linkun. Gene ontology analysis of the TNF signaling pathway in early orthodontic tooth movement of rats with periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(11): 695-702.
[12] GUO Xinwei,ZHAO Hongyan,YANG Yaoyao,QIAN Xin,LING Xiaoxu,ZHANG Zhimin. Research progress of periodontitis and periodontal dysbiosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(11): 739-744.
[13] CHENG Guoping,DING Yi,GUO Shujuan. Research progress on liposomes and periodontal disease [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(10): 658-661.
[14] CHEN Faming,GAO Lina,CHEN Fang. Current status and progress of periodontal regeneration therapy [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(1): 9-16.
[15] Yi DING,Qi WANG. Therapeutic progress of diabetic periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(9): 545-550.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Hong-chang LAI,Jun-yu SHI. Maxillary sinus floor elevation[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(1): 8 -12 .
[2] Pin ZHOU, Yang-fei LI. MRI study of temporomandibular joint disc position in asymptomatic volunteers[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(4): 239 -244 .
[3] Xinxin XIA, Fang FANG, Lijuan CHENG. Shaping ability of Pathfile and WaveOne in simulated root canals[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(6): 365 -368 .
[4] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560 -564 .
[5] Chengzhang LI. Masticatory muscles in occlusion[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 755 -760 .
[6] . [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 1 .
[7] Zhirong WU, Shiguang Huang. Research progress on the etiology, clinical examination and treatment of peri-implantitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(6): 401 -405 .
[8] Xiaowu YAO, Shisheng CHEN, Zizheng LU, Minxiao LIN. Clinical report and literature review on the amyloidosis of salivary glands[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 533 -536 .
[9] Lan LIAO, Lijun ZENG. Updated research on digitalization in aesthetic restoration[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 409 -414 .
[10] Yu LU, Chengxia LIU, Zhongjun LIU. Role of TRAF6 in inflammatory responses of human osteoblast-like cells with Enterococcusfaecalis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(7): 420 -425 .
This work is licensed under a Creative Commons Attribution 3.0 License.