Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (10): 639-643.doi: 10.12016/j.issn.2096-1456.2018.10.005

• Basic Study • Previous Articles     Next Articles

Significance of the levels of Twist and Vimentin proteins in oral squamous cell carcinoma

Wenfeng GAO1(),Zhiping WANG1,Jing XU1,Zhifeng SONG2(),Shuguang ZENG1()   

  1. 1. Department of oral and maxillofacial surgery, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China
    2. Shanghai Stomatological Hospital, Shanghai 200001, China
  • Received:2018-05-17 Revised:2018-07-03 Online:2018-10-20 Published:2018-10-20

Abstract:

Objective To investigate the levels of the Twist and Vimentin proteins in oral squamous cell carcinoma (OSCC) and analyze the clinical significance of Twist and Vimentin.Methods Eighty-five samples of OSCC and fifteen samples of normal oral mucosa were collected. Immunohistochemistry (SP method) was used to detect the expression of proteins, including Twist and vimentin. The relationship among these proteins and clinical pathological parameters was analyzed using SPSS statistical software.Results In the normal group, 13.3% (2/15) of samples were positive for the Twist protein; this value was significantly lower than that in OSCC group (80.0%, 66/85) (χ 2=26.98, P < 0.001). The expression of Twist was associated with clinical stage (χ 2=5.40, P=0.02) and lymph node metastasis (χ 2=8.35, P=0.006), while no correlations were found between the expression of Twist and sex (χ 2=0.23, P=0.63), age (χ 2= 0.31, P=0.58), location (χ 2=1.46, P=0.235) or degree of differentiation (χ 2=1.52, P=0.47). Additionally, 6.7% of samples (1/15) were positive for vimentin; this value was significantly lower than that in OSCC group (74.1%, 63/85) (χ 2=20.71, P < 0.001). The expression of vimentin was associated with clinical stage (χ 2=4.51, P=0.034) and lymph node metastasis (χ 2=6.75, P=0.009), while no correlations were found between the expression of vimentin and sex (χ 2=0.40, P=0.53), age (χ 2=0.17, P=0.68), location (χ 2=0.74,P=0.39) or degree of differentiation (χ 2=4.58, P=0.10). Spearman correlation analyses showed that Twist protein expression was positively correlated with vimentin (r=0.578, P<0.05). Conclusion Our data demonstrate that in OSCC, Twist and vimentin levels were upregulated, and Twist protein expression was positively correlated with vimentin, which indicates that both Twist and vimentin may be involved in the occurrence of OSCC.

Key words: Oral squamous cell carcinoma, Twist protein, Vimentin protein, Epithelial-mesenchymal transition, Immunohistochemistry

CLC Number: 

  • R783.2

Figure 1

The expression of Twist in the OSCC and normal groups (Immunohistochemical staining × 200)"

Table 1

The relationship between the expression of Twist and Vimentin with OSCC parameters"

因素 样本例数 Twist Vimentin
阴性 阳性 χ2 P 阴性 阳性 χ2 P
年龄
<50岁
≥ 50岁
38 7 31 0.31 0.58 9 29 0.17 0.68
47 11 36 13 34
性别

47 12 35 0.23 0.63 14 33 0.40 0.53
38 8 30 9 29
肿瘤部位

其它
49 12 37 1.46 0.23 15 34 0.74 0.39
36 5 31 8 28
临床分期
Ⅰ-Ⅱ期
Ⅲ-Ⅳ期
34 11 23 5.40 0.02 13 21 4.51 0.034
51 6 45 9 42
淋巴结转移

36 13 23 8.35 0.006 15 21 6.75 0.009
49 5 44 8 41
组织学分化


25 7 18 1.52 0.47 11 14 4.58 0.10
33 6 27 8 25
27 4 23 5 22

Figure 2

The expression of Vimentin in the OSCC and normal groups (Immunohistochemical staining × 200)"

[1] Khan MA, Chen HC, Zhang D , et al. Twist: a molecular target in cancer therapeutics[J]. Tumour Biol, 2013,34(5):2497-2506.
doi: 10.1007/s13277-013-1002-x pmid: 23873099
[2] Zou H, Feng X, Cao JG . Twist in hepatocellular carcinoma: pathophysiology and therapeutics[J]. Hepatol Int, 2015,9(3):399-405.
doi: 10.1007/s12072-015-9634-0 pmid: 26016463
[3] Li CW, Xia W, Lim SO , et al. AKT1 inhibits epithelial-to-mesenchymal transition in breast cancer through Phosphorylation-dependent twist1 degradation[J]. Cancer Res, 2016,76(6):1451-1462.
[4] 平金良, 顾栋桦, 徐炜 , 等. 喉鳞状细胞癌组织Twist表达与上皮-间质转化相关性分析[J]. 中华肿瘤防治杂志, 2015,22(18):1451-1455.
[5] Ozdemir A, Fisher-Aylor KI, Pepke S , et al. High resolution mapping of Twist to DNA in drosophila embryos: efficient functional analysis and evolutionary conservation[J]. Genome Res, 2011,21(4):566-577.
doi: 10.1101/gr.104018.109
[6] Cheng GZ, Chan J, Wang Q , et al. Twist transcriptionally up-regulates AKT2 in breast cancer cells leading to increased migration, invasion, and resistance to paclitaxel[J]. Cancer Res, 2007,67(5):1979-1987.
[7] Uthaya Kumar DB, Chen CL, Liu JC , et al. TLR4 signaling via NANOG cooperates with STAT3 to activate twist1 and promote formation of tumor-initiating stem-like cells in livers of mice[J]. Gastroenterology, 2016,150(3):707-719.
[8] Zhou Y, Zhang H, Zhuo X , et al. Over-expression of TWIST, an epithelial-mesenchymal transition inducer, predicts poor survival in patients with oral carcinoma[J]. Int J Clin Exp Med, 2015,8(6):9239-9247.
[9] 郑蜻, 南欣荣 . Twist蛋白在口腔鳞状细胞癌中的表达及意义[J]. 中国医药指南, 2012,10(24):212-213.
doi: 10.7666/d.y2126927
[10] Liu LK, Jiang XY, Zhou XX , et al. Upregulation of vimentin and aberrant expression of E-cadherin/beta-catenin complex in oral squamous cell carcinomas: correlation with the clinicopathological features and patient outcome[J]. Mod Pathol, 2010,23(2):213-224.
[11] Chaw SY, Abdul Majeed A, Dalley AJ , et al. Epithelial to mesenchymal transition (EMT) biomarkers--E-cadherin, beta-catenin, APC and vimentin--in oral squamous cell carcinogenesis and transformation[J]. Oral Oncol, 2012,48(10):997-1006.
doi: 10.1016/j.oraloncology.2012.05.011
[12] Imajyo I, Sugiura T, Kobayashi Y , et al. T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma[J]. Int J Oncol, 2012,41(6):1985-1995.
doi: 10.3892/ijo.2012.1673
[13] Dennis M, Wang G, Luo J , et al. Snail controls the mesenchymal phenotype and drives erlotinib resistance in oral epithelial and head and neck squamous cell carcinoma cells[J]. Otolaryngol Head Neck Surg, 2012,147(4):726-732.
[14] De Freitas Silva BS, Yamamoto-Silva FP, Pontes HA , et al. E-cadherin downregulation and Twist overexpression since early stages of oral carcinogenesis[J]. J Oral Pathol Med, 2014,43(2):125-131.
doi: 10.1111/jop.12096 pmid: 23772858
[15] 孙雅楠, 佘文婷, 薛敬玲 , 等. 口腔黏膜异常增生不同阶段上皮细胞E-cadherin,Twist1启动子甲基化研究[J]. 武汉大学学报:医学版, 2016,37(5):689-693.
[16] Gao Y, Xuan XY, Zhang HY , et al. Relationship between TWIST expression and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma[J]. Cell Biol Int, 2012,36(6):571-577.
doi: 10.1042/CBI20100195
[1] ZENG Fantao,YU Dongsheng. Knockdown of circ_0001273 inhibits the proliferation, migration and invasion of oral squamous cell carcinoma cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 153-157.
[2] CHEN Yi,XIE Hongliang,TANG Jianming,WENG Rulian,FENG Li,ZUO Min. Maxillofacial Rosai-Dorfman′s disease: a case report and literature review [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(2): 97-101.
[3] YIN Lifen,LIU Zhiwen,WU Hao,LING Tianyou. The expression of filaggrin in oral submucosal fibrosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(9): 557-560.
[4] LIN Lin,DUAN Ning,WANG Xiang,JIANG Hongliu,WANG Wenmei. Oral verrucous xanthoma in adolescents: a case report and literature review [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(5): 318-320.
[5] WANG Anxun. Abnormal glucose and lipid metabolism and oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 137-142.
[6] TIAN Yuanye,TANG Zhangui. Research progress on the CD4 +T cell balance in oral cancer and precancerous diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(2): 115-121.
[7] HUANG Lihuan,JIANG Yingtong,OUYANG Kexiong,WU Lihong,YANG Xuechao. Research progress on the role and mechanism of miR-155 in the development of oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 809-812.
[8] BUHAILIQIGULI Maimaitituersun,PATIGULI Wusiman,ADILI Moming. Peripheral primitive neuroectodermal tumor of the mandible: a case report and literature review [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 784-787.
[9] ZENG Sujuan,PENG Bo,CHENG Weidong,WEI Dongfeng,HUANG Wenyan,LI Yunyang,ZHAO Wanghong. Experimental study on the effect of hedysarum polybotys saccharides and selenizated hedysarum polybotys saccharides on oral squamous cancer cells in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 757-762.
[10] Lijuan ZHOU, Siming XIE. Progress in research on connexin 43 in oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(5): 338-340.
[11] Laiping ZHONG,Wutong JU. Progress of translational research on biomarker-based oral squamous cell carcinoma treatment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(10): 621-626.
[12] Huan-dong ZHANG, Si-ming XIE. The research progress of Gli1 in the regulation of epithelial mesenchymal transition in oral squamous cell car-cinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(5): 327-330.
[13] Zongsheng CHENG, Yinhuan JIN. Primary neuroendocrine small cell carcinoma of the parotid gland: A case report and literature review [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 803-805.
[14] Jingxu WANG, Guiqing LIAO. The analysis of expression of HAase in oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(10): 638-640.
[15] Yan-fei CHENG,Yong-rong ZHONG. Study of the cellular growth inhibited by tetrandrine with the expression of β-catenin in oral squamous cell carcinoma cell line [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(8): 464-468.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Hong-chang LAI,Jun-yu SHI. Maxillary sinus floor elevation[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(1): 8 -12 .
[2] Pin ZHOU, Yang-fei LI. MRI study of temporomandibular joint disc position in asymptomatic volunteers[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(4): 239 -244 .
[3] Xinxin XIA, Fang FANG, Lijuan CHENG. Shaping ability of Pathfile and WaveOne in simulated root canals[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(6): 365 -368 .
[4] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560 -564 .
[5] Chengzhang LI. Masticatory muscles in occlusion[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 755 -760 .
[6] . [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 1 .
[7] Zhirong WU, Shiguang Huang. Research progress on the etiology, clinical examination and treatment of peri-implantitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(6): 401 -405 .
[8] Xiaowu YAO, Shisheng CHEN, Zizheng LU, Minxiao LIN. Clinical report and literature review on the amyloidosis of salivary glands[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 533 -536 .
[9] Lan LIAO, Lijun ZENG. Updated research on digitalization in aesthetic restoration[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 409 -414 .
[10] Yu LU, Chengxia LIU, Zhongjun LIU. Role of TRAF6 in inflammatory responses of human osteoblast-like cells with Enterococcusfaecalis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(7): 420 -425 .
This work is licensed under a Creative Commons Attribution 3.0 License.