Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (10): 639-643.doi: 10.12016/j.issn.2096-1456.2018.10.005

• Basic Study • Previous Articles     Next Articles

Significance of the levels of Twist and Vimentin proteins in oral squamous cell carcinoma

Wenfeng GAO1(),Zhiping WANG1,Jing XU1,Zhifeng SONG2(),Shuguang ZENG1()   

  1. 1. Department of oral and maxillofacial surgery, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China
    2. Shanghai Stomatological Hospital, Shanghai 200001, China
  • Received:2018-05-17 Revised:2018-07-03 Online:2018-10-20 Published:2018-10-20

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Abstract:

Objective To investigate the levels of the Twist and Vimentin proteins in oral squamous cell carcinoma (OSCC) and analyze the clinical significance of Twist and Vimentin.Methods Eighty-five samples of OSCC and fifteen samples of normal oral mucosa were collected. Immunohistochemistry (SP method) was used to detect the expression of proteins, including Twist and vimentin. The relationship among these proteins and clinical pathological parameters was analyzed using SPSS statistical software.Results In the normal group, 13.3% (2/15) of samples were positive for the Twist protein; this value was significantly lower than that in OSCC group (80.0%, 66/85) (χ 2=26.98, P < 0.001). The expression of Twist was associated with clinical stage (χ 2=5.40, P=0.02) and lymph node metastasis (χ 2=8.35, P=0.006), while no correlations were found between the expression of Twist and sex (χ 2=0.23, P=0.63), age (χ 2= 0.31, P=0.58), location (χ 2=1.46, P=0.235) or degree of differentiation (χ 2=1.52, P=0.47). Additionally, 6.7% of samples (1/15) were positive for vimentin; this value was significantly lower than that in OSCC group (74.1%, 63/85) (χ 2=20.71, P < 0.001). The expression of vimentin was associated with clinical stage (χ 2=4.51, P=0.034) and lymph node metastasis (χ 2=6.75, P=0.009), while no correlations were found between the expression of vimentin and sex (χ 2=0.40, P=0.53), age (χ 2=0.17, P=0.68), location (χ 2=0.74,P=0.39) or degree of differentiation (χ 2=4.58, P=0.10). Spearman correlation analyses showed that Twist protein expression was positively correlated with vimentin (r=0.578, P＜0.05). Conclusion Our data demonstrate that in OSCC, Twist and vimentin levels were upregulated, and Twist protein expression was positively correlated with vimentin, which indicates that both Twist and vimentin may be involved in the occurrence of OSCC.

Key words: Oral squamous cell carcinoma, Twist protein, Vimentin protein, Epithelial-mesenchymal transition, Immunohistochemistry

CLC Number: 

  • R783.2

Figure 1

The expression of Twist in the OSCC and normal groups （Immunohistochemical staining × 200）"

Table 1

The relationship between the expression of Twist and Vimentin with OSCC parameters"

因素 样本例数 Twist Vimentin
阴性 阳性 χ2值 P值 阴性 阳性 χ2值 P值
年龄
<50岁
≥ 50岁
38 7 31 0.31 0.58 9 29 0.17 0.68
47 11 36 13 34
性别
男
女
47 12 35 0.23 0.63 14 33 0.40 0.53
38 8 30 9 29
肿瘤部位
舌
其它
49 12 37 1.46 0.23 15 34 0.74 0.39
36 5 31 8 28
临床分期
Ⅰ-Ⅱ期
Ⅲ-Ⅳ期
34 11 23 5.40 0.02 13 21 4.51 0.034
51 6 45 9 42
淋巴结转移
无
有
36 13 23 8.35 0.006 15 21 6.75 0.009
49 5 44 8 41
组织学分化
Ⅰ
Ⅱ
Ⅲ
25 7 18 1.52 0.47 11 14 4.58 0.10
33 6 27 8 25
27 4 23 5 22

Figure 2

The expression of Vimentin in the OSCC and normal groups （Immunohistochemical staining × 200）"

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