Journal of Prevention and Treatment for Stomatological Diseases ›› 2018, Vol. 26 ›› Issue (10): 639-643.doi: 10.12016/j.issn.2096-1456.2018.10.005

• Basic Study • Previous Articles     Next Articles

Significance of the levels of Twist and Vimentin proteins in oral squamous cell carcinoma

Wenfeng GAO1(),Zhiping WANG1,Jing XU1,Zhifeng SONG2(),Shuguang ZENG1()   

  1. 1. Department of oral and maxillofacial surgery, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China
    2. Shanghai Stomatological Hospital, Shanghai 200001, China
  • Received:2018-05-17 Revised:2018-07-03 Online:2018-10-20 Published:2018-10-20

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Abstract:

Objective To investigate the levels of the Twist and Vimentin proteins in oral squamous cell carcinoma (OSCC) and analyze the clinical significance of Twist and Vimentin.Methods Eighty-five samples of OSCC and fifteen samples of normal oral mucosa were collected. Immunohistochemistry (SP method) was used to detect the expression of proteins, including Twist and vimentin. The relationship among these proteins and clinical pathological parameters was analyzed using SPSS statistical software.Results In the normal group, 13.3% (2/15) of samples were positive for the Twist protein; this value was significantly lower than that in OSCC group (80.0%, 66/85) (χ 2=26.98, P < 0.001). The expression of Twist was associated with clinical stage (χ 2=5.40, P=0.02) and lymph node metastasis (χ 2=8.35, P=0.006), while no correlations were found between the expression of Twist and sex (χ 2=0.23, P=0.63), age (χ 2= 0.31, P=0.58), location (χ 2=1.46, P=0.235) or degree of differentiation (χ 2=1.52, P=0.47). Additionally, 6.7% of samples (1/15) were positive for vimentin; this value was significantly lower than that in OSCC group (74.1%, 63/85) (χ 2=20.71, P < 0.001). The expression of vimentin was associated with clinical stage (χ 2=4.51, P=0.034) and lymph node metastasis (χ 2=6.75, P=0.009), while no correlations were found between the expression of vimentin and sex (χ 2=0.40, P=0.53), age (χ 2=0.17, P=0.68), location (χ 2=0.74,P=0.39) or degree of differentiation (χ 2=4.58, P=0.10). Spearman correlation analyses showed that Twist protein expression was positively correlated with vimentin (r=0.578, P＜0.05). Conclusion Our data demonstrate that in OSCC, Twist and vimentin levels were upregulated, and Twist protein expression was positively correlated with vimentin, which indicates that both Twist and vimentin may be involved in the occurrence of OSCC.

Key words: Oral squamous cell carcinoma, Twist protein, Vimentin protein, Epithelial-mesenchymal transition, Immunohistochemistry

CLC Number: 

  • R783.2

Figure 1

The expression of Twist in the OSCC and normal groups （Immunohistochemical staining × 200）"

Table 1

The relationship between the expression of Twist and Vimentin with OSCC parameters"

因素 样本例数 Twist Vimentin
阴性 阳性 χ2值 P值 阴性 阳性 χ2值 P值
年龄
<50岁
≥ 50岁
38 7 31 0.31 0.58 9 29 0.17 0.68
47 11 36 13 34
性别
男
女
47 12 35 0.23 0.63 14 33 0.40 0.53
38 8 30 9 29
肿瘤部位
舌
其它
49 12 37 1.46 0.23 15 34 0.74 0.39
36 5 31 8 28
临床分期
Ⅰ-Ⅱ期
Ⅲ-Ⅳ期
34 11 23 5.40 0.02 13 21 4.51 0.034
51 6 45 9 42
淋巴结转移
无
有
36 13 23 8.35 0.006 15 21 6.75 0.009
49 5 44 8 41
组织学分化
Ⅰ
Ⅱ
Ⅲ
25 7 18 1.52 0.47 11 14 4.58 0.10
33 6 27 8 25
27 4 23 5 22

Figure 2

The expression of Vimentin in the OSCC and normal groups （Immunohistochemical staining × 200）"

[1] Khan MA, Chen HC, Zhang D , et al. Twist: a molecular target in cancer therapeutics[J]. Tumour Biol, 2013,34(5):2497-2506.
doi: 10.1007/s13277-013-1002-x pmid: 23873099
[2] Zou H, Feng X, Cao JG . Twist in hepatocellular carcinoma: pathophysiology and therapeutics[J]. Hepatol Int, 2015,9(3):399-405.
doi: 10.1007/s12072-015-9634-0 pmid: 26016463
[3] Li CW, Xia W, Lim SO , et al. AKT1 inhibits epithelial-to-mesenchymal transition in breast cancer through Phosphorylation-dependent twist1 degradation[J]. Cancer Res, 2016,76(6):1451-1462.
[4] 平金良, 顾栋桦, 徐炜 , 等. 喉鳞状细胞癌组织Twist表达与上皮-间质转化相关性分析[J]. 中华肿瘤防治杂志, 2015,22(18):1451-1455.
[5] Ozdemir A, Fisher-Aylor KI, Pepke S , et al. High resolution mapping of Twist to DNA in drosophila embryos: efficient functional analysis and evolutionary conservation[J]. Genome Res, 2011,21(4):566-577.
doi: 10.1101/gr.104018.109
[6] Cheng GZ, Chan J, Wang Q , et al. Twist transcriptionally up-regulates AKT2 in breast cancer cells leading to increased migration, invasion, and resistance to paclitaxel[J]. Cancer Res, 2007,67(5):1979-1987.
[7] Uthaya Kumar DB, Chen CL, Liu JC , et al. TLR4 signaling via NANOG cooperates with STAT3 to activate twist1 and promote formation of tumor-initiating stem-like cells in livers of mice[J]. Gastroenterology, 2016,150(3):707-719.
[8] Zhou Y, Zhang H, Zhuo X , et al. Over-expression of TWIST, an epithelial-mesenchymal transition inducer, predicts poor survival in patients with oral carcinoma[J]. Int J Clin Exp Med, 2015,8(6):9239-9247.
[9] 郑蜻, 南欣荣 . Twist蛋白在口腔鳞状细胞癌中的表达及意义[J]. 中国医药指南, 2012,10(24):212-213.
doi: 10.7666/d.y2126927
[10] Liu LK, Jiang XY, Zhou XX , et al. Upregulation of vimentin and aberrant expression of E-cadherin/beta-catenin complex in oral squamous cell carcinomas: correlation with the clinicopathological features and patient outcome[J]. Mod Pathol, 2010,23(2):213-224.
[11] Chaw SY, Abdul Majeed A, Dalley AJ , et al. Epithelial to mesenchymal transition (EMT) biomarkers--E-cadherin, beta-catenin, APC and vimentin--in oral squamous cell carcinogenesis and transformation[J]. Oral Oncol, 2012,48(10):997-1006.
doi: 10.1016/j.oraloncology.2012.05.011
[12] Imajyo I, Sugiura T, Kobayashi Y , et al. T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma[J]. Int J Oncol, 2012,41(6):1985-1995.
doi: 10.3892/ijo.2012.1673
[13] Dennis M, Wang G, Luo J , et al. Snail controls the mesenchymal phenotype and drives erlotinib resistance in oral epithelial and head and neck squamous cell carcinoma cells[J]. Otolaryngol Head Neck Surg, 2012,147(4):726-732.
[14] De Freitas Silva BS, Yamamoto-Silva FP, Pontes HA , et al. E-cadherin downregulation and Twist overexpression since early stages of oral carcinogenesis[J]. J Oral Pathol Med, 2014,43(2):125-131.
doi: 10.1111/jop.12096 pmid: 23772858
[15] 孙雅楠, 佘文婷, 薛敬玲 , 等. 口腔黏膜异常增生不同阶段上皮细胞E-cadherin,Twist1启动子甲基化研究[J]. 武汉大学学报:医学版, 2016,37(5):689-693.
[16] Gao Y, Xuan XY, Zhang HY , et al. Relationship between TWIST expression and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma[J]. Cell Biol Int, 2012,36(6):571-577.
doi: 10.1042/CBI20100195
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