Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (1): 9-16.doi: 10.12016/j.issn.2096-1456.2019.01.003

• Expert Forum • Previous Articles     Next Articles

Current status and progress of periodontal regeneration therapy

CHEN Faming(),GAO Lina,CHEN Fang   

  1. State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases& Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi′an 710032, China
  • Received:2018-07-05 Revised:2018-08-26 Online:2019-01-20 Published:2019-01-22
  • Contact: Faming CHEN E-mail:cfmsunhh@fmmu.edu.cn

Abstract:

Periodontitis is the most common oral disease and has a high incidence in humans. Periodontitis seriously affects the function of the oral and maxillofacial systems and is the most important cause of tooth loss in adults. In addition, as a long-term and persistent source of infection, periodontitis can not only trigger chronic inflammation and immune responses but also has an important impact on systemic health. Traditional periodontitis treatment focuses on inflammation control, and although this can prevent or delay the progression of the disease, satisfactory periodontal lesion tissue regeneration is difficult to obtain. With the introduction of new technologies and new materials such as guided tissue regeneration, bone grafting, growth factors and biological materials for the treatment of periodontitis, the method of periodontal tissue regeneration is more abundant, and the clinical effect has been greatly improved. In the future, stem cell transplantation and endogenous regeneration strategies are expected to become important methods for physiological and functional regeneration of periodontal lesions. This article briefly reviews the basic research and clinical application of periodontal tissue regeneration and forecasts the future development prospects and opportunities in this field.

Key words: Periodontitis, Guided tissue regeneration, Growth factors, Stem cell transplantation, Endogenous regeneration, Periodontal regeneration

CLC Number: 

  • R781.4

Table 1

Classification and characteristics of bone graft materials"

分类 来源 特性 缺点
自体骨 口腔内部 骨引导 可利用材料有限
口腔外部 骨诱导 可能需要二阶段手术
骨生成
同种异体骨 新鲜冷冻骨 骨引导 不能完全排除传播疾病的可能性
冻干骨 骨诱导
脱矿冻干骨
异种骨 牛骨 骨引导 所有产品均不能排除传播疾病的可能性
珊瑚 吸收缓慢
骨替代品 聚合物 骨引导 吸收能力不同
生物陶瓷
磷酸三钙
羟基磷灰石
生物活性玻璃
[1] Pihlstrom BL, Michalowicz BS, Johnson NW . Periodontal diseases[J]. Lancet, 2005,366(9499):1809-1820.
doi: 10.1016/S0140-6736(05)67728-8
[2] Chen FM, Zhang J, Zhang M , et al. A review on endogenous regenerative technology in periodontal regenerative medicine[J]. Biomaterials, 2010,31(31):7892-7927.
doi: 10.1016/j.biomaterials.2010.07.019
[3] Larsson L, Decker AM, Nibali L , et al. Regenerative medicine for periodontal and peri-implant diseases[J]. J Dent Res, 2016,95(3):255-266.
doi: 10.1177/0022034515618887
[4] Kuang YC, Hu B, Chen J , et al. Effects of periodontal endoscopy on the treatment of periodontitis: a systematic review and meta-analysis[J]. J Am Dent Assoc, 2017,148(10):750-759.
doi: 10.1016/j.adaj.2017.05.011
[5] Orekhova LY, Neizberg DM, Demchenko TV , et al. Prospects of endoscopic technology for diagnostics of inflammatory periodontal disease[J]. Stomatologiia (Mosk), 2016,95(5):4-7.
[6] Bundidpun P, Srisuwantha R, Laosrisin N . Clinical effects of photodynamic therapy as an adjunct to full-mouth ultrasonic scaling and root planing in treatment of chronic periodontitis[J]. Laser Ther, 2018,27(1):33-39.
doi: 10.5978/islsm.18-OR-03
[7] Mopur JM, Devi TR, Ali SM , et al. Clinical and radiographic evaluation of regenerative potential of GTR membrane (Biomesh ®) along with alloplastic bone graft (Biograft ®) in the treatment of periodontal intrabony defects [J]. J Contemp Dent Pract, 2013,14(3):434-439.
doi: 10.5005/jp-journals-10024
[8] Keles GC, Sumer M, Cetinkaya B , et al. Effect of autogenous cortical bone grafting in conjunction with guided tissue regeneration in the treatment of intraosseous periodontal defects[J]. Eur J Dent, 2010,4(4):403-411.
[9] Sculean A, Nikolidakis D, Nikou G , et al. Biomaterials for promoting periodontal regeneration in human intrabony defects: a systematic review[J]. Periodontol 2000, 2015,68(1):182-216.
doi: 10.1111/prd.12086
[10] Palachur D, Prabhakara RK, Murthy KR , et al. A comparative evaluation of bovine-derived xenograft (Bio-Oss Collagen) and type I collagen membrane(Bio-Gide)with bovine-derived xenograft (Bio-Oss Collagen) and fibrin fibronectin sealing system(TISSEEL)in the treatment of intrabony defects: a clinico-radiographic study[J]. J Indian Soc Periodontol, 2014,18(3):336-343.
doi: 10.4103/0972-124X.134572
[11] Heberer S, Al-Chawaf B, Hildebrand DA , et al. Histomorphometric analysis of extraction sockets augmented with Bio-Oss collagen after a 6-week healing period: a prospective study[J]. Clin Oral Implants Res, 2008,19(12):1219-1225.
doi: 10.1111/clr.2008.19.issue-12
[12] Oortgiesen DA, Plachokova AS, Geenen C , et al. Alkaline phosphatase immobilization onto Bio-Gide (R) and Bio-Oss (R) for periodontal and bone regeneration[J]. J Clin Periodontol, 2012,39(6):546-555.
doi: 10.1111/j.1600-051X.2012.01877.x
[13] Nagayasu-Tanaka T, Anzai J, Takaki S , et al. Action mechanism of fibroblast growth factor-2 (FGF-2) in the promotion of periodontal regeneration in Beagle dogs[J]. PLoS One, 2015,10(6):e0131870.
doi: 10.1371/journal.pone.0131870
[14] Cao Y, Liu ZH, Xie YL , et al. Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swine[J]. Stem Cell Res Ther, 2015,6:249.
doi: 10.1186/s13287-015-0244-5
[15] Lee J, Wikesjö UM . Growth/differentiation factor-5: pre-clinical and clinical evaluations of periodontal regeneration and alveolar augmentation--review[J]. J Clin Periodontol, 2014,41(8):797-805.
doi: 10.1111/jcpe.2014.41.issue-8
[16] Li FF, Yu FY, Xu X , et al. Evaluation of recombinant human FGF-2 and PDGF-BB in periodontal regeneration: a systematic review and Meta-analysis[J]. Sci Rep, 2017,7(1):65.
doi: 10.1038/s41598-017-00113-y
[17] Lo KW, Ulery BD, Ashe KM , et al. Studies of bone morphogenetic protein-based surgical repair[J]. Adv Drug Deliv Rev, 2012,64(12):1277-1291.
doi: 10.1016/j.addr.2012.03.014
[18] Calin C, Patrascu I . Growth factors and beta-tricalcium phosphate in the treatment of periodontal intraosseous defects: a systematic review and meta-analysis of randomised controlled trials[J]. Arch Oral Biol, 2016,66:44-54.
doi: 10.1016/j.archoralbio.2016.02.007
[19] Geiger M, Li RH, Friess W . Collagen sponges for bone regeneration with rhBMP-2[J]. Adv Drug Deliv Rev, 2003,55(12):1613-1629.
doi: 10.1016/j.addr.2003.08.010
[20] Hammarström L . Enamel matrix, cementum development and regeneration[J]. J Clin Periodontol, 1997,24(9):658-668.
doi: 10.1111/cpe.1997.24.issue-9
[21] Miron RJ, Sculean A, Cochran DL , et al. Twenty years of enamel matrix derivative: the past, the present and the future[J]. J Clin Periodontol, 2016,43(8):668-683.
doi: 10.1111/jcpe.12546
[22] Apicella A, Heunemann P, Dejace LA , et al. Scaffold requirements for periodontal regeneration with enamel matrix derivative proteins[J]. Colloids Surf B Biointerfaces, 2017,156:221-226.
doi: 10.1016/j.colsurfb.2017.05.013
[23] Ghezzi C, Ferrantino L, Bernardini L , et al. Minimally invasive surgical technique in periodontal regeneration: a randomized controlled clinical trial pilot study[J]. Int J Periodontics Restorative Dent, 2016,36(4):475-482.
doi: 10.11607/prd.2550
[24] Matarasso M, Iorio-Siciliano V, Blasi A , et al. Enamel matrix derivative and bone grafts for periodontal regeneration of intrabony defects. A systematic review and meta-analysis[J]. Clin Oral Investig, 2015,19(7):1581-1593.
doi: 10.1007/s00784-015-1491-7
[25] Viana Casarin RC, Ribeiro ED, Nociti J , et al. Enamel matrix derivative proteins for the treatment of proximal class Ⅱ furcation involvements: a prospective 24-month randomized clinical trial[J]. J Clin Periodontol, 2010,37(12):1100-1109.
doi: 10.1111/cpe.2010.37.issue-12
[26] Alexiou A, Vouros I, Menexes GA . Comparison of enamel matrix derivative (Emdogain) and subepithelial connective tissue graft for root coverage in patients with multiple gingival recession defects: a randomized controlled clinical study[J]. Quintessence Int (Berl), 2017,48(5):381-389.
[27] Rodella LF, Favero G, Boninsegna R , et al. Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction[J]. Microsc Res Tech, 2011,74(8):772-777.
doi: 10.1002/jemt.v74.8
[28] Masuki H, Okudera T, Watanebe T , et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF)[J]. Int J Implant Dent, 2016,2(1):19.
doi: 10.1186/s40729-016-0052-4
[29] Plachokova AS, Nikolidakis D, Mulder JA , et al. Effect of platelet-rich plasma on bone regeneration in dentistry: a systematic review[J]. Clin Oral Implants Res, 2008,19(6):539-545.
doi: 10.1111/j.1600-0501.2008.01525.x
[30] Roselló-Camps À, Monje A, Lin GH , et al. Platelet-rich plasma for periodontal regeneration in the treatment of intrabony defects: a meta-analysis on prospective clinical trials[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2015,120(5):562-574.
doi: 10.1016/j.oooo.2015.06.035
[31] Saleem M, Pisani F, Zahid FM , et al. Adjunctive platelet-rich plasma (PRP) in infrabony regenerative treatment: a systematic review and RCT's meta-analysis[J]. Stem Cells Int, 2018: 9594235.
[32] Thorat MK, Baghele ON, Rakhewar PS . Adjunctive effect of autologous platelet-rich fibrin in the treatment of intrabony defects in localized aggressive periodontitis patients: a randomized controlled split-mouth clinical trial[J]. Int J Periodont Restor Dent, 2017,37(6):302-309.
doi: 10.11607/prd.2972
[33] Miron RJ, Zucchelli G, Pikos MA , et al. Use of platelet-rich fibrin in regenerative dentistry: a systematic review[J]. Clin Oral Investig, 2017,21(6):1913-1927.
doi: 10.1007/s00784-017-2133-z
[34] Qiao J, Duan J, Zhang Y , et al. The effect of concentrated growth factors in the treatment of periodontal intrabony defects[J]. Future science OA, 2016, 2(4): FS136.
[35] Bartold PM, Mcculloch CA, Narayanan AS , et al. Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology[J]. Periodontol 2000, 2000,24(1):253-269.
doi: 10.1034/j.1600-0757.2000.2240113.x
[36] Ashri NY, Ajlan SA, Aldahmash AM . Dental pulp stem cells biology and use for periodontal tissue engineering[J]. Saudi Med J, 2015,36(12):1391-1399.
doi: 10.15537/smj.2015.12.12750
[37] Bright R, Hynes K, Gronthos S , et al. Periodontal ligament-derived cells for periodontal regeneration in animal models: a systematic review[J]. J Periodontal Res, 2015,50(2):160-172.
doi: 10.1111/jre.2015.50.issue-2
[38] Khorsand A, Eslaminejad MB, Arabsolghar M , et al. Autologous dental pulp stem cells in regeneration of defect created in canine periodontal tissue[J]. J Oral Implantol, 2013,39(4):433-443.
doi: 10.1563/AAID-JOI-D-12-00027
[39] Hu JC, Cao Y, Xie YL , et al. Periodontal regeneration in swine after cell injection and cell sheet transplantation of human dental pulp stem cells following good manufacturing practice[J]. Stem Cell Res Ther, 2016,7(1):130.
doi: 10.1186/s13287-016-0362-8
[40] Aimetti M, Ferrarotti F, Cricenti L , et al. Autologous dental pulp stem cells in periodontal regeneration: a case report[J]. Int J Periodont Restor Dent, 2014,34(Suppl):S27-S33.
[41] Tomasello L, Mauceri R, Coppola A , et al. Mesenchymal stem cells derived from inflamed dental pulpal and gingival tissue: a potential application for bone formation[J]. Stem Cell Res Ther, 2017,8(1):179.
doi: 10.1186/s13287-017-0633-z
[42] Li Y, Zhao SM, Nan X , et al. Repair of human periodontal bone defects by autologous grafting stem cells derived from inflammatory dental pulp tissues[J]. Stem Cell Res Ther, 2016,7(1):141.
doi: 10.1186/s13287-016-0404-2
[43] Fu XR, Jin LY, Ma P , et al. Allogeneic stem cells from deciduous teeth in treatment for periodontitis in miniature swine[J]. J Periodontol, 2014,85(6):845-851.
doi: 10.1902/jop.2013.130254
[44] Du J, Shan Z, Ma P , et al. Allogeneic bone marrow mesenchymal stem cell transplantation for periodontal regeneration[J]. J Dent Res, 2014,93(2):183-188.
doi: 10.1177/0022034513513026
[45] Tobita M, Mizuno H . Adipose-derived stem cells and periodontal tissue engineering[J]. Int J Oral Maxillofac Implants, 2013,28(6):e487-e493.
doi: 10.11607/jomi.te29
[46] Requicha JF, Viegas CA, Munoz F , et al. A tissue engineering approach for periodontal regeneration based on a biodegradable double-layer scaffold and adipose-derived stem cells[J]. Tissue Eng Part A, 2014,20(17/18):2483-2492.
doi: 10.1089/ten.tea.2013.0360
[47] Han J, Menicanin D, Marino V , et al. Assessment of the regenerative potential of allogeneic periodontal ligament stem cells in a rodent periodontal defect model[J]. J Periodontal Res, 2014,49(3):333-345.
doi: 10.1111/jre.2014.49.issue-3
[48] Ding G, Liu Y, Wang W , et al. Allogeneic periodontal ligament stem cell therapy for periodontitis in swine[J]. Stem Cells, 2010,28(10):1829-1838.
doi: 10.1002/stem.v28:10
[49] Liu Y, Zheng Y, Ding G , et al. Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine[J]. Stem Cells, 2008,26(4):1065-1073.
doi: 10.1634/stemcells.2007-0734
[50] Mrozik KM, Wada N, Marino VA , et al. Regeneration of periodontal tissues using allogenic periodontal ligament stem cells in an ovine model[J]. Regen Med, 2013,8(6):711-723.
doi: 10.2217/rme.13.66
[51] Ding G, Wang W, Liu Y , et al. Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla[J]. J Cell Physiol, 2010,223(2):415-422.
[52] Liu OS, Xu JJ, Ding G , et al. Periodontal ligament stem cells regulate B lymphocyte function via programmed cell death protein 1[J]. Stem Cells, 2013,31(7):1371-1382.
doi: 10.1002/stem.v31.7
[53] Kinnaird T, Stabile E, Burnett MS , et al. Local delivery of marrow-derived stromal cells augments collateral perfusion through paracrine mechanisms[J]. Circulation, 2004,109(12):1543-1549.
doi: 10.1161/01.CIR.0000124062.31102.57
[54] Baik HS, Park J, Lee KJ , et al. Local application of periodontal ligament stromal cells promotes soft tissue regeneration[J]. Oral Dis, 2014,20(6):574-581.
doi: 10.1111/odi.2014.20.issue-6
[55] Wei F, Qu C, Song T , et al. Vitamin C treatment promotes mesenchymal stem cell sheet formation and tissue regeneration by elevating telomerase activity[J]. J Cell Physiol, 2012,227(9):3216-3224.
doi: 10.1002/jcp.24012
[56] Owaki T, Shimizu T, Yamato M , et al. Cell sheet engineering for regenerative medicine: current challenges and strategies[J]. Biotechnol J, 2014,9(7):904-914.
doi: 10.1002/biot.201300432
[57] Nishida K, Yamato M, Hayashida Y , et al. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium[J]. N Engl J Med, 2004,351(12):1187-1196.
doi: 10.1056/NEJMoa040455
[58] Ohashi K, Yokoyama T, Yamato M , et al. Engineering functional two-and three-dimensional liver systems in vivo using hepatic tissue sheets[J]. Nat Med, 2007,13(7):880-885.
doi: 10.1038/nm1576
[59] Hasegawa M, Yamato M, Kikuchi A , et al. Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model[J]. Tissue Eng, 2005,11(3/4):469-478.
doi: 10.1089/ten.2005.11.469
[60] Akizuki T, Oda S, Komaki M , et al. Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs[J]. J Periodontal Res, 2005,40(3):245-251.
doi: 10.1111/jre.2005.40.issue-3
[61] Iwata T, Yamato M, Tsuchioka H , et al. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model[J]. Biomaterials, 2009,30(14):2716-2723.
doi: 10.1016/j.biomaterials.2009.01.032
[62] Kim JH, Ko SY, Lee JH , et al. Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets[J]. J Periodontal Implant Sci, 2017,47(6):402-415.
doi: 10.5051/jpis.2017.47.6.402
[63] Zhu M, Miao B, Zhu J , et al. Transplantation of periodontal ligament cell sheets expressing human β defensin3 promotes antiinflammation in a canine model of periodontitis[J]. Mol Med Rep, 2017,16(5):7459-7467.
doi: 10.3892/mmr.2017.7514
[64] Monsarrat P, Vergnes JN, Nabet C , et al. Concise review: mesenchymal stromal cells used for periodontal regeneration: a systematic review[J]. Stem Cells Transl Med, 2014,3(6):768-774.
doi: 10.5966/sctm.2013-0183
[65] Hu L, Liu Y, Wang S . Stem cell-based tooth and periodontal regeneration[J]. Oral Dis, 2017,24(5):12703.
[66] Chen FM, Gao LN, Tian BM , et al. Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial[J]. Stem Cell Res Ther, 2016,7:33.
doi: 10.1186/s13287-016-0288-1
[67] Li X, He XT, Yin Y , et al. Administration of signalling molecules dictates stem cell homing for in situ regeneration[J]. J Cell Mol Med, 2017,21(12):3162-3177.
doi: 10.1111/jcmm.2017.21.issue-12
[68] Yin Y, Li X, He XT , et al. Leveraging stem cell homing for therapeutic regeneration[J]. J Dent Res, 2017,96(6):601-609.
doi: 10.1177/0022034517706070
[1] WU Hongyu,MA Xiaoxin,LU Haixia,FENG Xiping,GU Qin,YE Wei,XIE Yingxin,XIE Danshu,WANG Wenji. Investigation of dental caries and periodontal conditions in maintenance hemodialysis patients [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 313-317.
[2] ZHANG Limu,LIN Xiaoping. Research progress on the mechanism of C-reactive protein mediated periodontitis and systemic diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 184-188.
[3] XIAO Wenlan,HU Chen,RONG Shengan,ZHU Chenyou,WU Yingying. Application of dentin in bone tissue engineering [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(2): 127-130.
[4] LI Jiatong,ZHOU Xuedong,XU Xin,WANG Yan. Research progress of probiotics in the prevention and treatment of oral infectious diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(9): 598-601.
[5] Gang WANG,Peng SUN,Juan LI,Shiguang HUANG. Expression of the receptor of advanced glycation end products in gingival tissue endothelial cells from type 2 diabetic rats with chronic periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 428-434.
[6] DAI Qian,YU Yuanling,HUANG Shiguang. Macrophage IL-33 expression in macrophages of human chronic periapical periodontitis and apical cyst [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(5): 300-303.
[7] LIU Qian,LAN Lufang,YAN Junyi,TIAN Weidong,GUO Shujuan. Research on the surface structure of a dentin matrix with complete demineralization and incomplete demineralization and the osteogenic property promotion of human periodontal ligament cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 159-166.
[8] XU Xiongcheng,ZHONG Quan,LUO Kai. Application of 3D printing technology in preparation of scaffolds for periodontal tissue regeneration [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 189-193.
[9] ZHU Yanxia,Gegen tana. Research progress of IL-33/ST2 signaling pathway in bone metabolism [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 202-204.
[10] WANG Ye,LIN Xiaoping. Research progress on the common risk factors and related mechanisms of periodontitis and osteoporosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 794-798.
[11] WANG Min, GE Song. Research progress on the T cell receptor CDR3 spectral sequence of periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 799-803.
[12] MA Yao,JIANG Zhaowei,JIN Yunyi,MIAO Qian,ZHANG Chunxiang,ZHANG Linkun. Gene ontology analysis of the TNF signaling pathway in early orthodontic tooth movement of rats with periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(11): 695-702.
[13] GUO Xinwei,ZHAO Hongyan,YANG Yaoyao,QIAN Xin,LING Xiaoxu,ZHANG Zhimin. Research progress of periodontitis and periodontal dysbiosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(11): 739-744.
[14] Yi DING,Qi WANG. Therapeutic progress of diabetic periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(9): 545-550.
[15] Zhenzhe LIU,Liang ZHAO,Huairong HUANG,Wenxia HUANG,Hongbai CHEN. Study of the Er: YAG laser in periodontal flap surgery for the treatment of chronic periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(9): 574-577.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] . [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(2): 125 -128 .
[2] Hong-chang LAI,Jun-yu SHI. Maxillary sinus floor elevation[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(1): 8 -12 .
[3] Pin ZHOU, Yang-fei LI. MRI study of temporomandibular joint disc position in asymptomatic volunteers[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(4): 239 -244 .
[4] Xinxin XIA, Fang FANG, Lijuan CHENG. Shaping ability of Pathfile and WaveOne in simulated root canals[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(6): 365 -368 .
[5] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560 -564 .
[6] Chengzhang LI. Masticatory muscles in occlusion[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 755 -760 .
[7] . [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 1 .
[8] Zhirong WU, Shiguang Huang. Research progress on the etiology, clinical examination and treatment of peri-implantitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(6): 401 -405 .
[9] Xiaowu YAO, Shisheng CHEN, Zizheng LU, Minxiao LIN. Clinical report and literature review on the amyloidosis of salivary glands[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 533 -536 .
[10] Lan LIAO, Lijun ZENG. Updated research on digitalization in aesthetic restoration[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 409 -414 .
This work is licensed under a Creative Commons Attribution 3.0 License.