Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (3): 153-158.doi: 10.12016/j.issn.2096-1456.2019.03.003

• Basic Study • Previous Articles     Next Articles

Preparation and antibacterial properties of La-doped TiO2 films on 3Y-TZP ceramic surface

ZENG Yongfa,FU Yulin(),DAI Qun,SHI Lianshui   

  1. The Affiliated Stomatology Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine, Nanchang 330006, China
  • Received:2018-04-26 Revised:2018-06-06 Online:2019-03-20 Published:2019-03-20
  • Contact: Yulin FU E-mail:13077963396@163.com

Abstract:

Objective To prepare the La-doped TiO2 film on the surface of 3Y-TZP ceramics in order to observe its antibacterial properties, providing an experimental basis for the application of antibacterial zirconia ceramics in the clinic.Method A cylindrical 3Y-TZP specimen with a diameter of 20 mm and a height of 3 mm was prepared. The sol-gel method was used to prepare 1% lanthanum (La)-doped TiO2 sol, and the La-TiO2 film-3Y-TZP ceramic was prepared by dip coating on the surface of 3Y-TZP. The TiO2 thin film-3Y-TZP ceramic was prepared by adding no lanthanum nitrate solution in the same way. The surface morphology of 3Y-TZP ceramic specimens (3Y-TZP group), TiO2 thin film-3Y-TZP ceramic specimens (TiO2 film group), and La-TiO2 thin film-3Y-TZP ceramic specimens (La-TiO2 film group) was observed by scanning electron microscopy, and photocatalysis, antibacterial and cytotoxicity experiments were carried out. For the photocatalytic experiment, four specimens were randomly selected from the La-TiO2 film group and TiO2 film group to observe the degradation rate of methylene blue at different time points under sunlight. In the antimicrobial experiment, five specimens were randomly selected from the La-TiO2 film group, TiO2 film group and 3Y-TZP group. The antimicrobial activity of each group was tested using the bacteriostatic circle method, and the bacteriostatic distance of each group was compared. In the cytotoxicity experiment, three samples were randomly selected from the La-TiO2 film group, TiO2 film group and 3Y-TZP group (negative control group), and the extracts were prepared. Here, 0.064% phenol solution were served as the positive control group. Morphological observation of L-929 cells in four groups was performed after 3 d of culture. Cell viability and relative cell proliferation were measured by MTT assay.Results La-TiO2 films with uniform distribution can be prepared on a 3Y-TZP surface by the dipping-drawing method. Photocatalytic experiments showed that the degradation rate of methylene blue in the La-TiO2 film group was (41.2 ± 1.5)% in daylight for 2 hours, which was higher than that in TiO2 film group (36.5 ± 2.4)%. A significant difference was noted between the two groups (t=3.321, P=0.016). The antimicrobial experiment showed that the antimicrobial distance of La-TiO2 group was (0.34 ± 0.08) mm, which was larger than that of TiO2 group (0.12 ± 0.02) mm. No obvious antimicrobial circle was noted in 3Y-TZP group, and the antimicrobial distance of the La-TiO2 film group was larger than that of TiO2 film group (F=63.798, P < 0.001). Cytotoxicity test showed that the relative proliferation rate of cells in La-TiO2 film group was (89.5 ± 1.3)%, and the cytotoxicity rating was grade 1. Significant differences were noted among the four groups (F=68.250, P < 0.001).Conclusion The La-doped TiO2 film can improve its photocatalytic activity and enhance the antibacterial activity of 3Y-TZP without obvious cytotoxicity.

Key words: 3Y-TZP ceramic, Zirconia ceramics, TiO2, La, Photocatalytic activity, Antibacterial, Cytotoxicity

CLC Number: 

  • R783.1

Table 1

Criteria for evaluating cytotoxicity[5]"

细胞相对增殖率 细胞毒性分级 材料的生物安全性评价
≥100% 0 合格
80%~99% 1 合格
50%~79% 2 综合细胞形态分析
30%~49% 3 不合格
0~29% 4 不合格

Figure 1

SEM images of the surface of specimens in 3 groups × 50 000"

Table 2

Methylene blue degradation rate of TiO2 and La-TiO2 films under the sun x ± s"

组别 0.5 h 1.0 h 1.5 h 2.0 h
TiO2 12.2 ± 1.6 21.7 ± 2.1 29.3 ± 1.8 36.5 ± 2.4
La-TiO2薄膜 15.7 ± 1.5 25.8 ± 1.3 35.4 ± 1.7 41.2 ± 1.5
t 3.568 3.712 5.509 3.871
P 0.007 0.006 <0.001 0.005

Figure 2

Zone of anti-bacteria test of 3 groups"

Figure 3

Morphological observation of L-929 cells after 3 d of cultivation in the four groups"

Table 3

Optical density value, RGR and cytotoxicity gradation after 3 d of cultivation in extracts x ± s"

组别 光密度值 相对增殖率(%) 毒性分级
3Y-TZP组 0.906 ± 0.214 100.0 ± 3.81 0
TiO2 薄膜组 0.830 ± 0.128 91.6 ± 4.69 1
La-TiO2 薄膜组 0.811 ± 0.196 89.5 ± 5.81 1
阳性对照租 0.170 ± 0.182 18.8 ± 2.73 4
F 23.582 68.250
P <0.001 <0.001
[1] Ristic M, Svabic MV, Sasic M , et al. Clinical and microbiological effects of fixed orthodontic appliances on periodontal tissues in adolescents[J]. Orthod Craniofac Res, 2007,10(4):187-195.
doi: 10.1111/j.1601-6343.2007.00396.x pmid: 17973685
[2] 霍媛媛, 韩轩, 李雨庆 , 等. 龋病相关微生物群落结构与功能的多组学研究进展[J]. 口腔疾病防治, 2018,26(3):195-199.
doi: 10.12016/j.issn.2096-1456.2018.03.011
[3] Desrousseaux C, Sautou V, Descamps S , et al. Modification of the surfacees of medical devices to prevent microbial adhesion and biofilm formation[J]. J Hosp Infect, 2013,85(2):87-93.
doi: 10.1016/j.jhin.2013.06.015 pmid: 24007718
[4] Krishnan S. Biofilm formation on medical devices and infection:preventive approaches//Biofilm and materials science[M]. Springer International Publishing, 2015: 93-108.
[5] 国家技术监督局 GB/T 14233.2-2005.. 第二部分:生物学实验方法[S] . 北京: 中国标准出版社, 2005.
[6] Ou SF, Huang MS, Chiou SY , et al. Research of antibacterial activity on silver containing yttria-stabilized-zirconia bioceramic[J]. Ceramics Int, 2013,39(4):3591-3596.
doi: 10.1016/j.ceramint.2012.10.186
[7] Reddy KR, Karthik KV, Prasad S , et al. Enhanced photocatalytic activity of nanostructured titanium dioxide/polyaniline hybrid photocatalysts[J]. Polyhedron, 2016,120(14):169-174.
doi: 10.1016/j.poly.2016.08.029
[8] Tsuang YH, Sun JS, Yc H , et al. Studies of photo killing of bacteria using titanium dioxide nanoparticles[J]. Artif Organs, 2008,32:167-174.
doi: 10.1111/j.1525-1594.2007.00530.x pmid: 18269355
[9] Mandari KK, Police AKR, Jy D , et al. Rare earth metal Gd influenced defect sites in N doped TiO2: defect mediated improved charge transfer for enhanced photocatalytic hydrogen production[J]. Int J Hydrogen Energy, 2018,43(4):2073-2082.
doi: 10.1016/j.ijhydene.2017.12.050
[10] Reszczyńska J, Grzyb T, Sobczaketal JW . Lanthanide co-doped TiO2: the effect of metal type and amount on surface properties and photocatalytic activity[J]. Appl Surf Sci, 2014,307:333-345.
doi: 10.1016/j.apsusc.2014.03.199
[11] Zhang ZG, Li GX, Cui ZJ , et al. Influence of difference quantity La-doped TiO2 photoanodes on the performance of dye-sensitized solar cells: a strategy for choosing an appropriate doping quantity[J]. J Solid State Chem, 2016,237:242-247.
doi: 10.1016/j.jssc.2016.02.005
[12] Mulwa W, Ouma CNM, Onani MO , et al. Energetic, electronic and optical propertyes of lanthanide doped TiO2: an ab initio LDA+U study[J]. J Solid State Chem, 2016,237:129-137.
doi: 10.1016/j.jssc.2016.02.003
[13] 薛寒松, 李华基, 胡慧芳 , 等. 镧掺杂二氧化钛纳米管光催化性能[J]. 中国稀土学报, 2008,26(1):18-23.
doi: 10.3321/j.issn:1000-4343.2008.01.004
[14] 吴亚惠, 龙明策, 蔡伟民 . TiO2纳米薄膜光催化剂制备技术的新进展[J]. 材料导报, 2010,24(9):45-49.
[15] 谭才邓, 朱美娟, 杜淑霞 , 等. 抑菌试验中抑菌圈法的比较研究[J]. 食品工业, 2016,37(11):122-125.
[16] Ong THD, Yu N, Meenashisundaram GK , et al. Insight into cytotoxicity of Mg nanocomposites using MTT assay technique[J]. Mater Sci Eng C, 2017,78:647-652.
doi: 10.1016/j.msec.2017.04.129 pmid: 28576033
[17] Uo M, Sjoren G, Sundh A , et al. Cytotoxicity and bonding property of dental ceramics[J]. Dent Mater, 2003,19(6):487-492.
doi: 10.1016/S0109-5641(02)00094-5 pmid: 12837396
[1] TAO Qian,HUANG Yun. Re-cognition of chronic obstructive diseases of the parotid gland [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 577-583.
[2] TANG Mingyue,HU Ao,CAI Bolei,GAO Ye,LIU Fuwei,LV Qianxin,JIN Dan,HOU Yan,WANG Le,ZHANG Zhouyang,KONG Liang. The effects of casein kinase 2 interacting protein-1 on the growth and development of craniomaxillofacial soft and hard tissues in mice [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 584-590.
[3] CHEN Hongxing,LIU Siyao,HUANG Yuting,PAN Shuang. Stress distribution of composite resin filling in Class I cavity of molars with different cavosurface angle [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 596-603.
[4] ZHANG Chongchong,XIN Yuqi,LIU Maopeng,YUAN Hong,CAO Zhongyi,ZHANG Jie. Comparison of clinical effects of the Yu flap and the Karapandzic flap in repairing greater than 2/3 lower lip defects [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 604-610.
[5] LI Fangfang,MENG Jian,ZHUANG Qianwei. Polydocanol percutaneous sclerotherapy for the treatment of giant venular malformation of the lips and cheeks in adults [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 611-616.
[6] JIA Jingbo,REN Juan,WANG Xiaoqin. Study on the transverse relationship of the mandible between different skeletal vertical patterns in patients with skeletal ClassⅠ malocclusion [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 617-623.
[7] CHEN Jing,CHEN Wenchuan. Research progress on tetrabutylammonium dihydrogen trifluoride as a substitute for hydrofluoric acid used for porcelain surface treatment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 629-633.
[8] HUANG Yannan,CHENG Lei. Research progress on nano-calcium phosphate modified dental materials for the prevention and treatment of dental pulp diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 634-637.
[9] ZHOU Zeying,ZHANG Jingyue,NIU Ju,LIU Dandan,ZHAO Wendi,LIU Xiaoqiu. Research progress on the antibacterial properties of dental resin materials [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 638-643.
[10] OU Yanzhen,LI Baosheng,CAI Qing,JIANG Jiayang,JIN Zhuohua,MENG Weiyan. The impact of masticatory dysfunction caused by occlusal disharmony on cognitive function [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(9): 644-648.
[11] WANG Yitian,WU Yinlong,YU Fanyuan,WU Fanzi,WANG Chenglin,YE Ling. Research on the color stability of Biodentine and MTA within the blood environment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 515-522.
[12] LUO Xiaona,LIU Xianghui,WANG Bo,LIU Xin,XIE Xiaohua. The effect of inhibiting p38 MAPK on the expression of genes related to enamel development in mice [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 529-534.
[13] ZHONG qijian,JIN Tingting,PENG Yu,CHEN Weixiong,LI Jinsong. The effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 535-540.
[14] LIU Li,ZHOU Yan,ZHANG Daling,WANG Yuanyuan. Two methods of treatment for skeletal Class Ⅲ malocclusion on airway changes before and after clinical research [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 541-547.
[15] LIU Yanjie,SONG Yong,CHEN Yucong,WANG Junlin. Observation of the curative effect on curettage combined with fenestration for the treatment of jaw classic ameloblastoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 548-552.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] ZHU Song-song, HU Jing. The application of distraction osteogenesis in the temporomandibular joint ankylosis and secondary dentofacial deformities[J]. journal1, 2016, 24(1): 6 -10 .
[2] XU Jing. The influence of the impacted mandibular third molar extraction on the distal periodontal tissue of the mandibular second molar[J]. journal1, 2016, 24(1): 11 -14 .
[3] ZHONG Jiang-long, PAN Ji-yang, CHEN Wei-liang. The evaluation of Eagle syndrome treatment by surgery combined with antidepressant therapy[J]. journal1, 2016, 24(1): 26 -28 .
[4] CHEN Xi, SUN Qin-zhou. The study of colorimetric board of porcelain fused to metal restoration for moderate to severe dental fluorosis[J]. journal1, 2016, 24(1): 33 -36 .
[5] OUYANG Ke-xiong1, LIANG Jun, ZOU Rui, LI Zhi-qiang, BAI Zhi-bao, PIAO Zheng-guo, ZHAO Jian-Jiang.. Ion Torrent RNA-Seq detection and analysis of the long non-coding RNA in tongue squamous cell carcinoma[J]. journal1, 2016, 24(1): 15 -19 .
[6] YU Pei, XUE Jing, ZHANG Xiao-wei, ZHENG Cang-shang. The influence of the roughness of zirconia ceramic surface on microbial attachment[J]. journal1, 2016, 24(1): 20 -25 .
[7] LIU Fang. Clinical assessment of two fissure sealant techniques in children’s dental caries prevention[J]. journal1, 2016, 24(1): 44 -45 .
[8] . [J]. journal1, 2016, 24(1): 49 -52 .
[9] LU Jian-rong, BAN Hua-jie, WANG Dai-you, ZHOU Hui-hui, LONG Ru, QIN Shu-hua. Clinical observation of sternocleidomastoid muscle flaps combined with artificial biological membrane reparing the defects after parotidectomy[J]. journal1, 2016, 24(1): 29 -32 .
[10] LI Bin, HE Xiao-ning, GAO Yuan, HU Yu-ping. Clinical analysis of pain after two kinds of apical stop preparation[J]. journal1, 2016, 24(1): 40 -43 .
This work is licensed under a Creative Commons Attribution 3.0 License.