Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (4): 219-225.doi: 10.12016/j.issn.2096-1456.2019.04.003

• Basic Study • Previous Articles     Next Articles

Distribution of oral Streptococcus mutans and its correlation with dental caries in children of Bortala Mongolian Autonomous Prefecture

YANG Ting,ZHANG Wanting,LI Beibei,DONG Ying,CAO Hongfei,ZHAO Jin()   

  1. Stomatological College, Xinjiang Medical University, Urumqi 830054, China
  • Received:2018-10-20 Revised:2018-11-13 Online:2019-04-20 Published:2019-04-28
  • Contact: Jin ZHAO E-mail:merryljin@sina.com

Abstract:

Objective To study the detection rate of Streptococcus mutans in oral cavities of 3-5-year-old Han, Uygur and Mongolian children in Bortala Mongolian Autonomous Prefecture, and the correlation between genotype and dental caries of preschool children.Methods Ninety children were randomly selected from the sample bank of children′s oral epidemiological survey data in the Bozhou area of Xinjiang. Forty-five children were included in the high caries group (more than 5 missing teeth), and 45 children were included in the noncaries group (0 missing teeth); each group comprised 15 children of each of the Han, Uygur and Mongolian nationalities. Plaque samples were collected and cultured with light saliva-bacillin agar medium and brain-heart infusion medium. Streptococcus mutans were cultured, and clinical isolates were further isolated and identified by Gram staining, biochemical identification and polymerase chain reaction. Genotype distribution was detected by random primer polymerase chain reaction.Results The detection rate of Streptococcus mutans in the 90 included children was 75.5%. The detection rate of Streptococcus mutans in the high caries group was 86.7%, which was significantly higher than that in the caries-free group (64.4%) (P=0.014). There was no significant difference in the distribution of Streptococcus mutans among Han, Uygur and Mongolian nationalities (P=0.457). A total of 549 clinical strains of Streptococcus mutans were obtained, and 113 different genotypes were found. In the high caries group, 61.5% carried more than one genotype of Streptococcus mutans, and 37.9% of the caries-free group had more than one genotype. The genetic polymorphism of Streptococcus mutans in the high caries group was significantly higher than that in the caries-free group (P=0.035). Spearman correlation analysis showed that there was a positive correlation between oral Streptococcus mutans gene polymorphism and caries sensitivity (r=0.258, P=0.034).Conclusion The distribution of Streptococcus mutans in children′s oral cavity in the Bozhou area was different between the high caries group and the caries-free group, but there was no difference among nationalities. Streptococcus mutans in the high caries group had more genotypes than those in the caries-free group. The genetic polymorphism of Streptococcus mutans might be related to the caries-causing ability of Streptococcus mutans.

Key words: Streptococcus mutans, Children, Dental plaque, Dental caries, Genotype, Gene polymorphisms, Ethnic, Correlation

Table 1

Primer sequence for PCR"

引物 序列 扩增片段(bp)
GtfB-F
GtfB-R
ACTACACTTTCGGGTGGCTTGG
CAGTATAAGCGCCAGTTTCATC
517
GbpB-F CAACAGAAGCACAACCATCA 104
GbpB-R GTCCACCATTACCCCAGT

Figure 1

Morphology of Streptococcus mutans"

Figure 2

Biochemical identification results of Streptococcus mutans"

Figure 3

PCR identification of the Streptococcus mutans gtfB gene"

Figure 4

PCR identification of the Streptococcus mutans gbpB gene"

Table 2

Detection of Streptococcus mutans in the S-ECC groups and the CF groups of children in the three ethnic groups n(%)"

组别 人数 变形链球菌检出[例(%)] χ2 P
汉族 30 21(70.0)
高龋组 15 12(80.0) 1.429 0.427
无龋组 15 9(60.0)
维吾尔族 30 22(73.3)
高龋组 15 13(86.7) 2.727 0.215
无龋组 15 9(60.0)
蒙古族 30 25(83.3)
高龋组 15 14(93.0) 2.160 0.330
无龋组 15 11(73.3)
合计 90 68(75.5)
高龋组 45 39(86.7) 6.016 0.014
无龋组 45 29(64.4)

Figure 5

Results of Streptococcus mutans AP-PCR"

Table 3

Comparison of gene polymorphisms of Streptococcus mutans in the S-ECC and CF groups of children of the three ethnic groups n(%)"

组别 人数 基因型数量 Z P
1 2 3 4
汉族
高龋组
21
12 6(50.0) 2(16.7) 4(33.3) 0(0) -1.196 0.232
无龋组 9 6(66.7) 3(33.3) 0(0) 0(0)
维吾尔族 22
高龋组 13 4(30.8) 7(53.8) 1(7.7) 1(7.7) -1.057 0.290
无龋组 9 5(55.5) 3(33.3) 1(11.1) 0(0)
蒙古族 25
高龋组 14 5(35.7) 5(35.7) 2(14.3) 2(14.3) -0.986 0.324
无龋组 11 7(63.6) 2(18.2) 2(18.2) 0(0)
合计
高龋组
68
39

15(38.5)

14(35.9)

7(17.9)

3(7.7)

-2.113

0.035
无龋组 29 18(62.1) 8(27.6) 3(10.3) 0(0)
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