Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (5): 300-303.doi: 10.12016/j.issn.2096-1456.2019.05.005

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Macrophage IL-33 expression in macrophages of human chronic periapical periodontitis and apical cyst

DAI Qian1,YU Yuanling2,HUANG Shiguang1,()   

  1. 1. School of Stomatology, Jinan University, Guangzhou 510632, China
    2. Jinan University First Affiliated Hospital, Guangzhou 510632, China
  • Received:2018-08-10 Revised:2018-10-12 Online:2019-05-20 Published:2019-05-20
  • Contact: Shiguang HUANG E-mail:thshg@126.com

Abstract:

Objective To observe the expression of interleukin-33 (IL-33) in macrophages of chronic periapical periodontitis and apical cyst tissue, and to provide a basis for the study of the pathogenesis of IL-33 in periapical diseases. Methods The apical tissues of 20 normal control group, 15 chronic periapical periodontitis group and 15 apical cyst group were collected for HE staining and optical microscopy respectively. CD14 was used as the marker of macrophages and double immunofluorescence staining was used to observe the changes of periapical tissues under fluorescence microscopy. The expression of IL-33 in CD14-positive macrophages was observed.Results The macrophage density (cell/mm 2) of IL-33 and CD14 positive expression in normal control group, chronic periapical periodontitis group and root cyst group were(23.81 ± 5.16,62.97 ± 8.54,119.83 ± 14.61) respectively, and there were significant differences among the three groups(F=87.17,P < 0.01). The density of IL-33 and CD14 positive macrophages in root cyst group was significantly higher than that in chronic periapical periodontitis group and control group(P < 0.01).Conclusion IL-33 and CD14 positive macrophages increased in normal apical tissue, chronic periapical periodontitis tissue and apical cyst tissue in turn.

Key words: Interleukin-33, Chronic periapical periodontitis, Periapical cysts, CD14, Macrophage

CLC Number: 

  • R781

Figure 1

Apical tissue hematoxylin-eosin staining bar=50 μm"

Figure 2

IL-33-CD14 double-positive macrophages infiltration as determined by double immunofluorescence staining in 3 groups using confocal laser scanning microscopy bar=20 μm"

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