Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (6): 350-354.doi: 10.12016/j.issn.2096-1456.2019.06.002

• Basic Study • Previous Articles     Next Articles

Mitochondrial fission protein 1 determines mitochondrial fission and cisplatin sensitivity in tongue squamous cell carcinoma

Xinyuan LEI,Xinyu LIN,Zhanpeng OU,Yi RUAN(),Jinsong LI()   

  1. Department of Stomatology, Sun Yat-sen Memorial hospital, Sun Yat-sen University,Guangzhou 510120, China
  • Received:2019-03-07 Revised:2019-04-02 Online:2019-06-20 Published:2019-06-21
  • Contact: Yi RUAN,Jinsong LI E-mail:13802929651@139.com;lijinsong1967@163.com

Abstract:

Objective To investigate the effect of mitochondrial fission protein 1 (FIS1) on apoptosis and cisplatin resistance in tongue squamous cell carcinoma (TSCC) cells. Methods The squamous cell carcinoma cell lines SCC9 and CAL27 were used to detect the mRNA and protein levels of FIS1 after cisplatin treatment, the knockdown and overexpression of FIS1 of SCC9 and CAL27 with or without cisplatin treatment were accomplished through small interfering RNA (siRNA) and plasmid, respectively. The mitochondrial division state in cells was detected by mitochondrial staining, and the apoptosis state of cells was detected by TUNEL, flow cytometry and Caspase 3/7. Results FIS1 protein expression in tongue squamous carcinoma cells treated with cisplatin was increased, but the mRNA level did not change. Silencing of FIS1 expression reduced mitochondrial division and apoptosis in squamous cell carcinoma cells treated with cisplatin, whereas overexpression of FIS1 exhibited the opposite effects. The percentage of dividing mitochondria, the number of apoptotic cells and the activity of Caspase 3/7 in SCC9 and CAL27 cells were significantly different before and after modulation of FIS1 expression (P < 0.05). Conclusion FIS1 is involved in the regulation of cisplatin chemotherapy sensitivity in tongue squamous cell carcinoma and can be used as a new target for improving the sensitivity of cisplatin chemotherapy in oral squamous cell carcinoma.

Key words: Mitochondrial dynamics, Tongue squamous cell carcinoma, Cisplatin sensitivity, FIS1, Chemotherapy, Apoptosis, Small interfere RNA

CLC Number: 

  • R739.8

Figure 1

Expression of FIS1 and mitochondrial fission in tongue squamous cell carcinoma cells treated with cisplatin "

Figure 2

Silencing FIS1 reversed cisplatin-induced mitochondrial fission and apoptosis "

Figure 3

Overexpression of FIS1 promoted mitochondrial fission and apoptosis"

[1] Soares CD, Morais TML, Mariano FV , et al. Expression of mitochondrial dynamics markers during melanoma progression: comparative study of head and neck cutaneous and mucosal melanomas[J]. J Oral Pathol Med. 2019. doi: 10.1111/jop.
[2] Mishra P, Chan DC . Mitochondrial dynamics and inheritance during cell division, development and disease[J]. Nat Rev Mol Cell Biol, 2014,15(10):634-646.
[3] Galluzzi L, Senovilla L, Vitale I , et al. Molecular mechanisms of cisplatin resistance[J]. Oncogene, 2012,31(15):1869-1883.
[4] Zhong LP, Zhang CP, Ren GX , et al. Randomized phase III trial of induction chemotherapy with docetaxel, cisplatin, and fluorouracil followed by surgery versus Up-Front surgery in locally advanced resectable oral squamous cell carcinoma[J]. J Clin Oncol, 2013,31(6):744-751.
doi: 10.1200/JCO.2012.43.8820
[5] Wang JX, Jiao JQ, Li Q , et al. miR-499 regulates mitochondrial dynamics by targeting calcineurin and dynamin-related protein-1[J]. Nat Med, 2011,17(1):71-78.
[6] Tanaka A, Youle RJ . A chemical inhibitor of DRP1 uncouples mitochondrial fission and apoptosis[J]. Mol Cell, 2008,29(4):409-410.
doi: 10.1016/j.molcel.2008.02.005
[7] Srinivasan S, Guha M, Kashina A , et al. Mitochondrial dysfunction and mitochondrial dynamics-the cancer connection[J]. Biochim Biophys Acta Bioenerg, 2017,1858(8, SI):602-614.
doi: 10.1016/j.bbabio.2017.01.004
[8] Trotta AP, Chipuk JE . Mitochondrial dynamics as regulators of cancer biology[J]. Cell Mol Life Sci, 2017,74(11):1999-2017.
doi: 10.1007/s00018-016-2451-3
[9] Zorzano A , Isabel Hernandez-Alvarez M, Sebastian D , et al. Mitofusin 2 as a driver that controls energy metabolism and insulin signaling[J]. Antioxid Redox Signal, 2015,22(12):1020-1031.
doi: 10.1089/ars.2014.6208
[10] Li J, Wang YL, Wang YP , et al. Pharmacological activation of AMPK prevents Drp1-mediated mitochondrial fission and alleviates endoplasmic reticulum stress-associated endothelial dysfunction[J]. J Mol Cell Cardiol, 2015,86:62-74.
doi: 10.1016/j.yjmcc.2015.07.010
[11] Kashatus JA, Nascimento A, Myers LJ , et al. Erk2 phosphorylation of Drp1 promotes mitochondrial fission and MAPK-driven tumor growth[J]. Mol Cell. 2015,57(3):537-551.
doi: 10.1016/j.molcel.2015.01.002
[12] Wan YY, Zhang JF, Yang ZJ , et al. Involvement of Drp1 in hypoxia-induced migration of human glioblastoma U251 cells[J]. Oncol Rep, 2014,32(2):619-626.
doi: 10.3892/or.2014.3235
[13] Friedman JR, Lackner LL, West M , et al. ER tubules mark sites of mitochondrial division[J]. Science, 2011,334(6054):358-362.
doi: 10.1126/science.1207385
[1] WU Fayin,XU Haili. Effect and mechanism of allicin combined with 5-fluorouracil on proliferation and apoptosis of the MEC-1 cell line in mucoepidermoid carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 355-360.
[2] ZOU Xiaolong,CHEN Yuan,WANG Yan,WANG Jiantao. Research progress on animal models of oral mucositis caused by radiotherapy and chemotherapy [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 322-326.
[3] LI Xiaodong,MENG Jian. Application progress of recombinant human endostatin in the treatment of head and neck cancer [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(4): 267-272.
[4] ZHOU Jiaqi,SHU Linjing,XIONG Yi,ZHANG Yixin,XIANG Lin,WU Yingying. Study on the role of FoxO1 in the regulation of osteoblastic metabolism by 1,25(OH)2D3 in a high glucose environment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(1): 24-29.
[5] JI Tong. Diagnosis and treatment of head and neck osteosarcoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(9): 545-550.
[6] Xinyu LIN,Weixiong CHEN,Xinyuan LEI,Zhanpeng OU,song FAN,Jinsong LI. Screening and identification of mitochondrial miRNAs related to chemotherapy resistance in tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 417-422.
[7] HE Yue,LI Xiaoguang. Prevention and treatment of osteoradionecrosis of the jaws [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 143-152.
[8] ZENG Sujuan,PENG Bo,CHENG Weidong,WEI Dongfeng,HUANG Wenyan,LI Yunyang,ZHAO Wanghong. Experimental study on the effect of hedysarum polybotys saccharides and selenizated hedysarum polybotys saccharides on oral squamous cancer cells in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 757-762.
[9] Chaobin PAN. Research progress in comprehensive and sequential treatment of tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(5): 273-280.
[10] Lijuan ZHOU, Siming XIE. Progress in research on connexin 43 in oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(5): 338-340.
[11] Songhao YIN, Weihong GUO. Investigation of the expression of TPX2 in tongue squamous cell carcinoma and its effect on Cal27 cell proliferation and apoptosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 31-37.
[12] Ya-nan WANG, Hao LIU, Jun ZHANG. Expression of autophagy-related proteins PTEN and MAP1LC3 in tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(5): 294-299.
[13] Huan-ling WANG, Jian-ping ZHANG. Effects of arecoline on apoptosis of human periodontal ligament fibroblasts [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(4): 223-226.
[14] Xiao-zhi LÜ,Ting-ru SHAO,Meng ZHAO. Reconstruction of defects with supraclavicular artery island flap following ablation of tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(7): 386-389.
[15] Li-na YU,Wen-xuan ZHANG,Yang YU,Wen-guang QIN,Xue-qi GUO. Influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(6): 336-340.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Hong-chang LAI,Jun-yu SHI. Maxillary sinus floor elevation[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(1): 8 -12 .
[2] Pin ZHOU, Yang-fei LI. MRI study of temporomandibular joint disc position in asymptomatic volunteers[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(4): 239 -244 .
[3] Xinxin XIA, Fang FANG, Lijuan CHENG. Shaping ability of Pathfile and WaveOne in simulated root canals[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(6): 365 -368 .
[4] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560 -564 .
[5] Chengzhang LI. Masticatory muscles in occlusion[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 755 -760 .
[6] . [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 1 .
[7] Zhirong WU, Shiguang Huang. Research progress on the etiology, clinical examination and treatment of peri-implantitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(6): 401 -405 .
[8] Xiaowu YAO, Shisheng CHEN, Zizheng LU, Minxiao LIN. Clinical report and literature review on the amyloidosis of salivary glands[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 533 -536 .
[9] Lan LIAO, Lijun ZENG. Updated research on digitalization in aesthetic restoration[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 409 -414 .
[10] Yu LU, Chengxia LIU, Zhongjun LIU. Role of TRAF6 in inflammatory responses of human osteoblast-like cells with Enterococcusfaecalis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(7): 420 -425 .
This work is licensed under a Creative Commons Attribution 3.0 License.