Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (6): 355-359.doi: 10.12016/j.issn.2096-1456.2019.06.003

• Basic Study • Previous Articles     Next Articles

The role of APOD in the proliferation and migration of human dental pulp cells

XU Shuaimei,ZENG Xiongqun,YUAN Peiyan,LIU Zhongjun(),ZENG Shuguang()   

  1. Departments of Endodontics, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China.
  • Received:2018-11-05 Revised:2019-01-06 Online:2019-06-20 Published:2019-06-21
  • Contact: Zhongjun LIU,Shuguang ZENG E-mail:153093518@qq.com;sunrisezdoctor@163.com

Abstract:

Objective To explore the role of apoliprotein D (APOD) in the proliferation and migration of human dental pulp cells (DPCs) and to provide a basis for the use of APOD to promote pulp regeneration. Methods APOD expression in human dental pulp cells was inhibited by siRNA. The inhibition effect of APOD was confirmed by qPCR and Western blot. After APOD inhibition, colony formation experiments and CCK8 assays were employed to confirm the proliferation ability of dental pulp cells. Transwell assays were used to verify the cell migration ability after the inhibition of APOD expression. Results After inhibiting APOD expression, the colony formation rate in the si-apod group was reduced compared with the NC group, and the difference was statistically significant (t=7.624, P=0.002). The CCK8 experiment showed that the OD value in the si-apod group decreased at 3, 5 and 7 d compared with that in the NC group (P < 0.05). Transwell results showed that the number of cell divisions was 57.25 ± 4.03 in the si-apod group and 154.50 ± 8.39 in the NC group, and the difference was statistically significant (t=10.45, P < 0.001). Conclusion Inhibition of APOD expression in dental pulp cells inhibits their proliferation and migration ability.

Key words: Apolipoprotein D, Pulp tissue regeneration, Dental pulp cells, Proliferation, Migration, CCK8

CLC Number: 

  • R781.3

Figure 1

Dental pulp cells were derived from dental pulp tissue explants × 100"

Figure 2

RNA and protein expression levels following transfection with siRNA in dental pulp cells"

Figure 3

Colony formation experiment"

Figure 4

CCK8 assay for detecting the proliferative capacity of dental pulp cells"

Figure 5

Transwell assay for detecting migration of dental pulp cells "

[1] Allina DO, Andreeva YY, Zavalishina LE , et al. Estimation of the diagnostic potential of APOD, PTOV1, and EPHA4 for prostatic neoplasms[J]. Arkh Patol, 2016,78(5):9-14.
[2] Gao GQ, Song LS, Tong B , et al. Expression levels of GSTA2 and APOD genes might be associated with carotenoid coloration in golden pheasant(Chrysolophus pictus) plumage[J]. Dongwuxue Yanjiu, 2016,37(3):144-150.
[3] Nazarenko MS, Markov AV, Sleptsov AA , et al. Comparative analysis of gene expression in vascular cells of patients with advanced atherosclerosis[J]. Biomed Khim, 2018,64(5):416-422.
doi: 10.18097/pbmc20186405416
[4] Sarjeant JM, Lawrie A, Kinnear C , et al. Apolipoprotein D inhibits platelet-derived growth factor-BB-induced vascular smooth muscle cell proliferated by preventing translocation of phosphorylated extracellular signal regulated kinase 1/2 to the nucleus[J]. Arterioscler Thromb Vasc Biol, 2003,23(12):2172-2177.
doi: 10.1161/01.ATV.0000100404.05459.39
[5] Xu S, Cui L, Ma D , et al. Effect of ITGA5 down-regulation on the migration capacity of human dental pulp stem cells[J]. Int J Clin Exp Pathol, 2015,8(11):14425-14432.
[6] Waldner A, Dassati S, Redl B , et al. Apolipoprotein D concentration in human plasma during aging and in parkinson′s disease: a cross-sectional Study[J]. Parkinsons Dis, 2018,2018:3751516.
[7] Sasaki Y, Negishi H, Koyama R , et al. p53 family members regulate the expression of the apolipoprotein D gene[J]. J Biol Chem, 2009,284(2):872-883.
doi: 10.1074/jbc.M807185200
[8] Lai CJ, Cheng HC, Lin CY , et al. Activation of liver X receptor suppresses angiogenesis via induction of ApoD[J]. FASEB J, 2017,31(12):5568-5576.
doi: 10.1096/fj.201700374R
[9] Martineau C, Najyb O, Signor C , et al. Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice[J]. Metabolism, 2016,65(9):1247-1258.
doi: 10.1016/j.metabol.2016.05.007
[10] Jin GZ, Kim HW . Co-culture of human dental pulp stem cells and endothelial cells using porous biopolymer microcarriers: a feasibility study for bone tissue engineering[J]. Tissue Eng Regen Med, 2017,14(4):393-401.
doi: 10.1007/s13770-017-0061-2
[11] Cui L, Xu SM, Ma DD , et al. The role of integrin-alpha 5 in the proliferation and odontogenic differentiation of human dental pulp stem cells[J]. J Endod, 2014,40(2):235-240.
doi: 10.1016/j.joen.2013.08.011
[12] Cui L, Xu SM, Ma DD , et al. The effect of TRPM7 suppression on the proliferation, migration and osteogenic differentiation of human dental pulp stem cells[J]. Int Endod J, 2014,47(6):583-593.
doi: 10.1111/iej.2014.47.issue-6
[13] Liu Y, Gao Y, Zhan X , et al. TLR4 activation by lipopolysaccharide and Streptococcus mutans induces differential regulation of proliferation and migration in human dental pulp stem cells[J]. J Endod, 2014,40(9):1375-1381.
doi: 10.1016/j.joen.2014.03.015
[14] Soares DG, Rosseto HL, Scheffel DS , et al. Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold[J]. Clin Oral Investig, 2017,21(9):2827-2839.
doi: 10.1007/s00784-017-2085-3
[15] Sarjeant JM, Lawrie A, Kinnear C , et al. Apolipoprotein D inhibits platelet-derived growth factor-BB-induced vascular smooth muscle cell proliferated by preventing translocation of phosphorylated extracellular signal regulated kinase 1/2 to the nucleus[J]. Arterioscler Thromb Vasc Biol, 2003,23(12):2172-2177.
doi: 10.1161/01.ATV.0000100404.05459.39
[16] Lv T, Wu Y, Mu C , et al. Insulin-like growth factor 1 promotes the proliferation and committed differentiation of human dental pulp stem cells through MAPK pathways[J]. Arch Oral Biol, 2016,72:116-123.
doi: 10.1016/j.archoralbio.2016.08.011
[17] Aksel H, Huang GT . Combined effects of vascular endothelial growth factor and bone morphogenetic protein 2 on odonto/osteogenic differentiation of human dental pulp stem cells in vitro[J]. J Endod, 2017,43(6):930-935.
doi: 10.1016/j.joen.2017.01.036
[18] Nobes CD, Hall A . Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia[J]. Cell, 1995,81(1):53-62.
doi: 10.1016/0092-8674(95)90370-4
[19] Kaibuchi K, Kuroda S, Amano M . Regulation of the cytoskeleton and cell adhesion by the Rho family GTPases in mammalian cells[J]. Annu Rev Biochem, 1999,68:459-486.
doi: 10.1146/annurev.biochem.68.1.459
[1] WU Fayin,XU Haili. Effect and mechanism of allicin combined with 5-fluorouracil on proliferation and apoptosis of the MEC-1 cell line in mucoepidermoid carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 355-360.
[2] ZENG Fantao,YU Dongsheng. Knockdown of circ_0001273 inhibits the proliferation, migration and invasion of oral squamous cell carcinoma cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 153-157.
[3] LIAN Keqian,ZHANG Xin,ZHOU Jieyu,LIAO Yanfen,SI Shanshan. Biocompatibility of bone marrow mesenchymal cells on polyetheretherketone and titanium surfaces in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(2): 73-78.
[4] ZHOU Jiaqi,SHU Linjing,XIONG Yi,ZHANG Yixin,XIANG Lin,WU Yingying. Study on the role of FoxO1 in the regulation of osteoblastic metabolism by 1,25(OH)2D3 in a high glucose environment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(1): 24-29.
[5] HE Jialin, XU Yan, XIE Xianzhe, WANG Tengfei, HUO Dongmei. Effect of platelet-rich fibrin extract on the proliferation of gingival fibroblasts [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(8): 490-495.
[6] Zehong GUO,Yingyuan NING,Shulan XU,Peijun ZHU,Xianglong DING,Yan GAO. Effect of laser-etched pure titanium surface on early proliferation of MG63 cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 435-440.
[7] ZHONG Wende,LIU Rongjing,GUAN Hongbing,CHEN Guangsheng. Research on the inhibitory effect of Baicalin on the human tongue squamous cell carcinoma cell line SCC15 [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 226-230.
[8] WANG Anxun. Abnormal glucose and lipid metabolism and oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 137-142.
[9] ZENG Sujuan,PENG Bo,CHENG Weidong,WEI Dongfeng,HUANG Wenyan,LI Yunyang,ZHAO Wanghong. Experimental study on the effect of hedysarum polybotys saccharides and selenizated hedysarum polybotys saccharides on oral squamous cancer cells in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 757-762.
[10] LU Weiying,LIU Ping,CHEN Jiawei,LIU Shuying,XU Pingping. Effects of X-ray irradiation on proliferation and RANTES expression of the mouse osteogenic precursor cell line MC3T3-E1 [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(10): 621-626.
[11] Qiaoli NIU, Yiming LI, Yanyan SONG, Chenxi LI, Jin ZHAO. Effects of different concentrations of MTA on the proliferation and differentiation of stem cells from the apical papilla [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 491-495.
[12] Xiaojin WANG, Xinchao YOU, Kai CHEN, Kunsong HUANG, Xuan PAN. Influence of luteolin on the invasion and migration of an human tongue squamous carcinoma cell line [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 434-439.
[13] Songhao YIN, Weihong GUO. Investigation of the expression of TPX2 in tongue squamous cell carcinoma and its effect on Cal27 cell proliferation and apoptosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 31-37.
[14] Fei HE, Yuyan ZHENG, Wei QIU, Guoquan ZHANG, Sui MAI. Effect of Notch signaling and autophagy on MTA induced differentiation of human dental pulp cells in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(7): 414-419.
[15] Jun WEN,Shuai-mei XU,Ying LIU. Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(8): 459-463.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Hong-chang LAI,Jun-yu SHI. Maxillary sinus floor elevation[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(1): 8 -12 .
[2] Pin ZHOU, Yang-fei LI. MRI study of temporomandibular joint disc position in asymptomatic volunteers[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(4): 239 -244 .
[3] Xinxin XIA, Fang FANG, Lijuan CHENG. Shaping ability of Pathfile and WaveOne in simulated root canals[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(6): 365 -368 .
[4] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560 -564 .
[5] Chengzhang LI. Masticatory muscles in occlusion[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 755 -760 .
[6] . [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 1 .
[7] Zhirong WU, Shiguang Huang. Research progress on the etiology, clinical examination and treatment of peri-implantitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(6): 401 -405 .
[8] Xiaowu YAO, Shisheng CHEN, Zizheng LU, Minxiao LIN. Clinical report and literature review on the amyloidosis of salivary glands[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(8): 533 -536 .
[9] Lan LIAO, Lijun ZENG. Updated research on digitalization in aesthetic restoration[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(7): 409 -414 .
[10] Yu LU, Chengxia LIU, Zhongjun LIU. Role of TRAF6 in inflammatory responses of human osteoblast-like cells with Enterococcusfaecalis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(7): 420 -425 .
This work is licensed under a Creative Commons Attribution 3.0 License.