Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (9): 551-556.doi: 10.12016/j.issn.2096-1456.2019.09.002

• Basic Study • Previous Articles     Next Articles

Experimental study of periostin promoting rapid distraction osteogenesis of the rabbit mandible

JIANG Xiaowen(),HUANG Huaqing,CHEN Jinyong,PENG Haiyan   

  1. Department of Stomatology, the First People’s Hospital of Chenzhou City, the South Medical University & Institute of Translation Medicine, University of China South, Chenzhou 423000, China
  • Received:2019-01-16 Revised:2019-04-03 Online:2019-09-20 Published:2019-09-20
  • Contact: Xiaowen JIANG E-mail:jxw0927@163.com

Abstract:

Objective To explore the promoting effect of periostin on rapid distraction osteogenesis of the rabbit mandible and provide experimental evidence for the clinical use of periostin to promote osteogenesis. Methods Twenty-four New Zealand male white rabbits underwent distraction osteogenesis, and after 3 days of retention, they were rapidly stretched at a stretch rate of 2 mm/d (total 5 d). The animals were randomly divided into group A and group B (12 per group). On the last day of the stretch, 0.5 mL of normal saline containing 40 μg of recombinant periostin was given to group B or an equal volume of normal saline was added to the control group (group A) for 8 days. At 4 weeks and 8 weeks post-stretch, 8 animals were randomly selected from each group to undergo a CT scan under general anesthesia. The bone mineral density and bone mineral content were detected by dual energy X-ray absorptiometry. Eight weeks post-stretch, all of the experimental animals were sacrificed. Six animals were randomly selected from each group for micro-CT and a histological examination, and the remaining animals were subjected to biomechanical tests. Results CT images showed that the new bone formation in the distraction space of group B was significantly better than that of group A at 4 and 8 weeks post-stretch. At 4 weeks and 8 weeks post-stretch, the bone mineral density in group B was (0.157 ± 0.016) g/cm 2 and (0.234 ± 0.023) g/cm 2, respectively, and the bone mineral content was (0.096 ± 0.010) g and (0.204 ± 0.017) g, respectively. The above four means were significantly higher in group B than in group A (P < 0.001). The micro-CT images and data suggest that the stretch gap microstructure of group B has more mature features. Histological experiments showed that the trabecular bone of group B was thick and mature, with few chondrocytes. The biomechanical test results showed that the biomechanical strength of the distraction gap in group B was (228.47 ± 39.98) N, which was 1.24 times that of group A (P = 0.045). Conclusion Interstitial use of periosteal protein in the distraction space of the mandible in rabbits can promote local new bone formation and mineralization.

Key words: periostin, rabbit, mandible, distraction osteogenesis, bone regeneration, bone mineralization density, bone mineral content

CLC Number: 

  • R78

Figure 1

Contrast images of a two-dimensional reconstruction of the distraction-side mandible in the two groups 4 weeks and 8 weeks after distraction"

Table 1

Comparison of the bone mineralization density and bone mineral content in the two groups 4 and 8 weeks after distraction"

组别 骨矿含量(g) 骨矿密度(g/cm2
4周 8周 4周 8周
A组 0.068 ± 0.012 0.122 ± 0.013 0.112 ± 0.008 0.176 ± 0.014
B组 0.096 ± 0.010 0.204 ± 0.017 0.157 ± 0.016 0.234 ± 0.023
F 24.662 126.943 50.663 37.347
P <0.001 <0.001 <0.001 <0.001

Figure 2

Comparison of pictures of micro-CT and the histological section eight weeks after distraction in the two groups"

Table 2

Comparison of the micro-CT parameters of the distraction gaps eight weeks after distraction in the two"

组别 BV/TV(%) Conn.D(mm-3 Tb.Th(mm) Tb.SP(mm) Tb.N(mm-1
A组 38.71 ± 4.52 17.31 ± 1.06 0.22 ± 0.03 0.20 ± 0.03 1.98 ± 0.09
B组 53.29 ± 6.79 19.79 ± 1.50 0.33 ± 0.05 0.29 ± 0.04 2.03 ± 0.08
F 19.464 10.875 20.188 20.704 0.823
P 0.001 0.008 0.001 0.001 0.386
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