Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (9): 557-560.doi: 10.12016/j.issn.2096-1456.2019.09.003

• Basic Study • Previous Articles     Next Articles

The expression of filaggrin in oral submucosal fibrosis

YIN Lifen1,LIU Zhiwen2,WU Hao1,LING Tianyou2()   

  1. 1.Department of Periodontal & Oral Medicine, Changsha Stomatological Hospital, Changsha 410004, China
    2.Department of Stomatology, Second Xiangya Hospital, Central South University, Changsha 410008, China
  • Received:2018-11-19 Revised:2019-03-29 Online:2019-09-20 Published:2019-09-20
  • Contact: Tianyou LING E-mail:772660166@qq.com

Abstract:

Objective To study the expression and distribution of filaggrin (FLG) in oral submucous fibrosis (OSF) and to explore the significance of FLG in the occurrence and development of OSF.Methods Ten cases with a normal oral mucosa (normal buccal mucosa group) and 30 cases of tissues with OSF lesions, including 10 cases each in the early (early OSF group), moderate (middle OSF group) and advanced stages (late OSF group), were selected. FLG was analyzed by immunohistochemistry. The FLG-positive cells were counted to calculate the percentages of cells with FLG-positive expression in each group.Results FLG expression was negative in most of the normal buccal mucosa group specimens and was positive in the OSF buccal mucosal epithelial specimens. With aggravation of the OSF lesion, the number of FLG-positive cells increased. In the early OSF group, FLG-positive expression was mainly concentrated in the granular and keratinized epithelial layers. In the middle OSF group, the number of FLG-positive epithelial cells increased gradually. In the late OSF group, almost all epithelial cells were FLG-positive in the cytoplasmic nucleus. The percentages of FLG-positive cells in the early, middle and late OSF groups were (24.63 ± 9.06)%, (54.23 ± 10.63)% and (83.97 ± 8.72)%, respectively. The percentage of FLG-positive cells was significantly higher in the OSF group than in the normal mucosa group (P < 0.05).Conclusion FLG was expressed at a higher level in the OSF epithelium than in the normal oral mucosal epithelium and was upregulated in the OSF epithelium with aggravation of the OSF lesions. Abnormal FLG expression may be related to the terminal differentiation disorder of OSF epithelial keratinocytes.

Key words: oral buccal mucosa, oral submucous fibrosis, filaggrin, immunohistochemistry, keratinocyte

CLC Number: 

  • R781.5

Figure 1

Immunohistochemical staining for filaggrin in each group bar=100 μm"

Table 1

Expression rates of FLG positive cells in each group $\bar{x}$ ± s,%"

组别 例数 阳性细胞表达率
正常口腔黏膜组 10 1.8±1.42
OSF早期组 10 24.63±9.061)
OSF中期组 10 54.23±10.631)2)
OSF晚期组 10 83.72±8.721)3)
F 185.73
P <0.05
[1] Bari S, Metgud R, Vyas Z , et al. An update on studies on etiological factors, disease progression, and malignant transformation in oral submucous fibrosis[J]. J Cancer Res Ther, 2017,13(3):399-405.
[2] Wollina U, Verma SB, Ali FM , et al. Oral submucous fibrosis: an update[J]. Clin Cosmet Investig Dermatol, 2015,8:193-204.
[3] Biradar SB, Munde AD, Biradar BC . Oral submucous fibrosis: a clinico-histopathological correlational study[J]. J Cancer Res Ther, 2018,14(3):597-603.
[4] Arakeri G, Brennan PA . Oral submucous fibrosis: an overview of the aetiology, pathogenesis, classification, and principles of management[J]. Br J Oral Maxillofac Surg, 2013,51(7):587-593.
[5] Arakeri G, Rai KK, Hunasgi S , et al. Oral submucous fibrosis: an update on current theories of pathogenesis[J]. J Oral Pathol Med, 2017,46(6):406-412.
[6] Hassani B, Isaian A, Shariat M . Filaggrin gene polymorphisms in Iranian ichthyosis vulgaris and atopic dermatitis patients[J]. Int J Dermatol, 2018,57(12):1485-1491.
[7] Oh Y, Lim HW, Kim K , et al. Ginsenoside Re improves skin barrier function in HaCaT keratinocytes under normal growth conditions[J]. Biosci Biotechnol Biochem, 2016,80(11):2165-2167.
[8] Cabanillas B, Novak N . Inactivation of mitogen-activated protein kinase signaling pathway reduces caspase-14 expression in impaired keratinocytes[J]. Curr Opin Immunol, 2016,19(1):28-33.
[9] Smith SA, Dale BA . Immunologic localization of filaggrin in human oral epithelia and correlation with keratinization[J]. J Investig Dermatol, 1986,86(2):168-172.
[10] Grosso M, Lentini M, Bellizi DME , et al. Immunohistochemical localization of filaggrin in benign and malignant lesions of the human oral mucosa[J]. Basic Appl Histochem, 1990,34(1):51-58.
[11] 李宁, 翦新春 . 兜甲蛋白和细胞色素P4503A5口腔黏膜下纤维化中的表达及意义[J]. 华西口腔医学杂志, 2009,27(1):29-33.
[12] Makino T, Mizawa M, Inoue S , et al. The expression profile of filaggrin-2 in the normal and pathologic human oral mucosa[J]. Arch Dermatol Res, 2016,308(3):213-217.
[13] Larsen KR, Johansen JD, Reibe J , et al. Filaggrin gene mutations and the distribution of filaggrin in oral mucosa of patients with oral lichen planus and healthy controls[J]. J Eur Acad Dermatol Venereol, 2017,31(5):887-893.
[14] Young CA, Eckert RL, Adhikary G , et al. Embryonic AP1 transcription factor deficiency causes a collodion baby-like phenotype[J]. J Invest Dermatol, 2017,137(9):1868-1877.
[15] Young CA, Rorke EA . Loss of epidermal AP1 transcription factor function reduces filaggrin level, alters chemokine expression and produces an ichthyosis-related phenotype[J]. Cell Death Dis, 2017,8(6):e2840.
[16] Jang SI, Steinert PM, Markova NG . Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene[J]. J Biol Chem, 1996,271(39):24105-24114.
[17] Lee SE, Lee SH . Skin barrier and calcium[J]. Ann Dermatol, 2018,30(3):265-275.
[18] Yuspa SL, Kilkenny AE, Steinert PM , et al. Expression of murine epideral differentiation lnarkers is tightly regulated by restricted extracellular calcium concentrations in vitro[J]. J Cell Biol, 1989,109(3):1207-1217.
[19] Murakami H, Okamura K, Aoki S , et al. Association of caspase-14 and filaggrin expression with keratinization of the oral mucosa and reconstruction culture rat models[J]. J Periodontal Res, 2014,49(6):703-710.
[20] Ranganathan K, Kavitha R, Sawant SS , et al. Cytokeratin expression in oral submucous fibrosis--an immunohistochemical study[J]. J Oral Pathol Med, 2006,35(1):25-32.
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