Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (10): 652-657.doi: 10.12016/j.issn.2096-1456.2019.09.008

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Research progress on exosomes derived from dental pulp stem cells

HE Hongzhi1,MA Dandan1,2()   

  1. 1. School of Stomatology, Southern Medical University, Guangzhou 510515, China
    2. Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Received:2019-04-17 Revised:2019-05-22 Online:2019-10-20 Published:2019-10-20
  • Contact: Dandan MA E-mail:mdd@smu.edu.cn

Abstract:

Exosomes are bilayer lipid vesicles formed by a series of regulatory processes including endocytosis, fusion and efflux. Exosomes can be secreted by a variety of cells, including epithelial cells, mast cells, dendritic cells, T lymphocytes, and mesenchymal stem cells. Mesenchymal stem cells play an important role in tissue repair and regeneration, immune regulation, and tumor growth regulation and have a strong secretory capacity. The therapeutic mechanism of mesenchymal stem cells is related to their exosomes. Although this therapeutic mechanism is not fully understood, it has shown strong therapeutic potential. Dental pulp stem cells are undifferentiated mesenchymal stem cells that present in dental pulp tissue. Their use has many advantages, such as a lack of ethical controversy, easy access, and good safety. Recent studies have found that exosomes secreted by dental pulp stem cells have great potential for applications in pulp regeneration, anti-inflammation treatment, neurodegenerative diseases and tumors. This article reviews the synthesis, secretion, extraction and identification of exosomes, the biological functions of mesenchymal stem cell exosomes, and the related research on dental pulp stem cell exosomes and diseases to improve clinicians′ understanding of the mechanism of the occurrence and development of dental pulp stem cell exosomes in diseases. Treatment and further research of these diseases provide new ideas and targets.

Key words: mesenchymal stem cells, dental pulp stem cells, exosomes, dental pulp regeneration, nerve regeneration, inflammation, gene vector

CLC Number: 

  • R781
[1] Chen G, Huang A, Zhang W , et al. Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response[J]. Nature, 2018,560(7718):382-386.
[2] Yeo RW, Lai RC, Zhang B , et al. Mesenchymal stem cell: an efficient mass producer of exosomes for drug delivery[J]. Adv Drug Deliv Rev, 2013,65(3):336-341.
[3] Zomer A, Maynard C, Verweij FJ , et al. In vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior[J]. Cell, 2015,161(5):1046-1057.
[4] Pugholm LH, Revenfeld AL, Jorgensen MM . Antibody-based assays for phenotyping of extracellular vesicles[J]. Biomed Res Int, 2015: 524817.
[5] 张灏, 赵立波, 叶国栋 . 外泌体研究,转化和临床应用专家共识[J]. 转化医学杂志, 2018,7(6):321-325.
[6] Van Niel G .D′angelo G, raposo G. shedding light on the cell biology of extracellular vesicles[J]. Nat Rev Mol Cell Biol, 2018,19(4):213-228.
[7] Jeppesen DK, Fenix AM, Franklin JL , et al. Reassessment of exosome composition[J]. Cell, 2019,177(2):428-445.
[8] Lötvall J, Hill AF, Hochberg F , et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the international society for extracellular vesicles[J]. J Extracell Vesicles, 2014,3:26913.
[9] Edgar JR, Eden ER, Futter CE . Hrs- and CD63-Dependent competing mechanisms make different sized endosomal intraluminal vesicles[J]. Traffic, 2014,15(2):197-211.
doi: 10.1111/tra.12139
[10] Chen L, Chen RJ, Kemper S , et al. Pathways of production and delivery of hepatocyte exosomes[J]. J Cell Commun Signal, 2018,12(1):343-357.
[11] Reza-Zaldivar EE, Hernandez-Sapiens MA, Minjarez B , et al. Potential effects of MSC-Derived exosomes in neuroplasticity in alzheimer′s disease[J]. Front Cell Neurosci, 2018,12:317.
[12] Mcbride JD, Rodriguez-Menocal L, Guzman W , et al. Bone marrow mesenchymal stem Cell-Derived CD63(+) exosomes transport Wnt3a exteriorly and enhance dermal fibroblast proliferation, migration, and angiogenesis in vitro[J]. Stem Cells Dev, 2017,26(19):1384-1398.
[13] Chen WC, Huang YK, Han JC , et al. Immunomodulatory effects of mesenchymal stromal cells-derived exosome[J]. Immunol Res, 2016,64(4):831-840.
[14] Jiang XF, Lew KS, Chen QY , et al. Human mesenchymal stem cell-derived exosomes reduce ischemia/reperfusion injury by the inhibitions of apoptosis and autophagy[J]. Curr Pharm Des, 2018,24(44):5334-5341.
[15] Arslan F, Lai RC, Smeets MB , et al. Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury[J]. Stem Cell Res, 2013,10(3):301-312.
[16] Yan YM, Jiang WQ, Tan YW , et al. hucMSC exosome-derived GPX1 is required for the recovery of hepatic oxidant injury[J]. Mol Ther, 2017,25(2):465-479.
[17] Hu L, Wang J, Zhou X , et al. Exosomes derived from human adipose mensenchymal stem cells accelerates cutaneous wound healing via optimizing the characteristics of fibroblasts[J]. Sci Rep, 2016,6:32993.
[18] Yang YY, Bucan V, Baehre H , et al. Acquisition of new tumor cell properties by MSC-derived exosomes[J]. Int J Oncol, 2015,47(1):244-252.
[19] Roccaro AM, Sacco A, Maiso P , et al. BM mesenchymal stromal cell-derived exosomes facilitate multiple myeloma progression[J]. J Clin Invest, 2013,123(4):1542-1555.
doi: 10.1172/JCI66517
[20] 叶青松, 王晓燕 . 牙源性干细胞储存和临床应用的研究进展[J]. 口腔疾病防治, 2018,26(1):15-25.
[21] 杨懋彬, 曾倩 . 牙髓根尖周病的治疗新方法:再生牙髓病学之路[J]. 口腔疾病防治, 2017,25(2):74-79.
[22] Dissanayaka WL, Hargreaves KM, Jin LJ , et al. The interplay of dental pulp stem cells and endothelial cells in an injectable peptide hydrogel on angiogenesis and pulp regeneration in vivo[J]. Tissue Eng Part A, 2015,21(3/4):550-563.
[23] 柳鑫, 肖燕, 江川 , 等. 牙髓干细胞来源外泌体诱导内皮细胞血管生成能力的研究[J]. 牙体牙髓牙周病学杂志, 2018,28(4):187-196.
[24] Huang CC, Narayanan R, Alapati S , et al. Exosomes as biomimetic tools for stem cell differentiation: applications in dental pulp tissue regeneration[J]. Biomaterials, 2016,111:103-115.
[25] Pivoraite U, JarmalaviČiūte A, Tunaitis V , et al. Exosomes from human dental pulp stem cells suppress carrageenan-induced acute inflammation in mice[J]. Inflammation, 2015,38(5):1933-1941.
[26] 苏晓磊, 汪坤, 刘月 , 等. 牙髓干细胞来源外泌体对急性肺损伤的作用及机制研究[J]. 军事医学, 2018,42(2):130-137.
[27] Li Y, Yang YY, Ren JL , et al. Exosomes secreted by stem cells from human exfoliated deciduous teeth contribute to functional recovery after traumatic brain injury by shifting microglia M1/M2 polarization in rats[J]. Stem Cell Res Ther, 2017,8(1):198.
[28] Carvalho IM, Coelho PB, Costa PC , et al. Current neurogenic and neuroprotective strategies to prevent and treat neurodegenerative and neuropsychiatric disorders[J]. Neuromolecular Med, 2015,17(4):404-422.
[29] Haney MJ, Klyachko NL, Zhao Y , et al. Exosomes as drug delivery vehicles for Parkinson's disease therapy[J]. J Control Release, 2015,207:18-30.
[30] Doeppner TR, Herz J, Goergens AA , et al. Extracellular vesicles improve Post-Stroke neuroregeneration and prevent postischemic immunosuppression[J]. Stem Cells Transl Med, 2015,4(10):1131-1143.
[31] Venugopal C, Shobha K, Rai KS , et al. Neuroprotection by human dental pulp mesenchymal stem cells: from billions to Nano[J]. Curr Gene Ther, 2018,18(5):307-323.
[32] Jarmalaviciute A, Tunaitis V, Pivoraite UA , et al. Exosomes from dental pulp stem cells rescue human dopaminergic neurons from 6-hydroxy-dopamine-induced apoptosis[J]. Cytotherapy, 2015,17(7):932-939.
[33] Altaner C, Altanerova V, Cihova M , et al. Complete regression of glioblastoma by mesenchymal stem cells mediated prodrug gene therapy simulating clinical therapeutic scenario[J]. Int J Cancer, 2014,134(6):1458-1465.
[34] Altanerova U, Benejova K, Altanerova V , et al. Dental pulp mesenchymal stem/stromal cells labeled with Iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma[J]. Neoplasma, 2016,63(6):925-933.
[35] Altanerova U, Babincova M, Babinec P , et al. Human mesenchymal stem cell-derived Iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia[J]. Int J Nanomedicine, 2017,12:7923-7936.
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