Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (12): 757-762.doi: 10.12016/j.issn.2096-1456.2019.12.002

• Basic Study • Previous Articles     Next Articles

Experimental study on the effect of hedysarum polybotys saccharides and selenizated hedysarum polybotys saccharides on oral squamous cancer cells in vitro

ZENG Sujuan1,2,PENG Bo2,CHENG Weidong3,WEI Dongfeng4,HUANG Wenyan2,LI Yunyang2,ZHAO Wanghong1()   

  1. 1. Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    2. Department of Pediatric Dentistry, Stomatological hospital affiliated to Guangzhou Medical University, Guangzhou 510140, China
    3. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
    4. Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2019-06-29 Revised:2019-08-28 Online:2019-12-20 Published:2019-12-30
  • Contact: Wanghong ZHAO E-mail:zhaowh@smu.edu.cn

Abstract:

Objective To study the effects of hedysarum polybotys saccharides (HPS) and selenizated hedysarum polybotys saccharides (SE-HPS) on the oral squamous cancer cell line SCC25. Methods Different concentrations (0, 10, 25, 50, 100, 200, 400 μg/ml) of HPS and SE-HPS were added to SCC25 cells in the logarithmic growth stage. Cell proliferation was detected by the CCK-8 method, apoptosis was detected by flow cytometry, and apoptosis-related indexes were observed by RT-qPCR and Western blotting. Results The concentrations of HPS and SE-HPS inhibited the proliferation of SCC25 cells. The inhibitory effect of 50 μg/mL HPS and SE-HPS on the proliferation of SCC25 cells was the strongest and was time-dependent. The inhibition effect significantly increased within 48 h, and the effect was achieved after 48 h. At the plateau stage, SE-HPS inhibited the proliferation of SCC25 cells more strongly than HPS (P < 0.05). The results of flow cytometry showed that 50 μg/mL HPS and SE-HPS acted on SCC25 cells for 48 h, and the apoptotic rates were 25.8% and 30.8% respectively. Compared with the control group (0 μg/mL HPS and SE-HPS), the difference was statistically significant (P < 0.05). RT-qPCR and Western blotting showed that 50 μg/mL HPS and SE-HPS acted on SCC25 cells for 48 h, and the mRNA and protein expression levels of the apoptosis gene Fas/FasL were upregulated. The difference was statistically significant (P < 0.05). Conclusion Both HPS and SE-HPS can inhibit the proliferation of SCC25 oral cancer cells, but SE-HPS is superior to HPS and can induce apoptosis through the Fas/Fasl pathway.

Key words: hedysarum polybotys saccharides, selenizated hedysarum polybotys saccharides, oral squamous cell carcinoma SCC25, experiment in vitro, proliferation, apoptosis, Fas/Fasl gene

CLC Number: 

  • R78

Table 1

Primer sequences"

基因 前引物 (5′-3′) 后引物(5′-3′)
β-actin TGGCACCCAGACAATGAA CCAGCCAAAGAAGAAACCAC
Fas GGTGGCTTTGTCTTCTTCT CCTTGGTTTTCCTTTCTGT
Fasl GGAGGGGAAGATGATGAG AGCACTGGTAAGATTGAACAC

Figure 1

Comparison of different concentrations of HPS on SCC25 apoptosis"

Figure 2

Comparison of different concentrations of SE-HPS on SCC25 apoptosis"

Figure 3

Effects of different concentrations of HPS and SE-HPS on Fas and Fasl expression"

Figure 4

Changes in Fas and Fasl protein expression in SCC25 cells treated with different concentrations of HPS and SE-HPS"

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