Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (12): 763-768.doi: 10.12016/j.issn.2096-1456.2019.12.003

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Study on the dental plaque microbial community in preschool children with different caries sensitivity

XIAO Xiaofen1,HE Shandan1,CHEN Yongyi1,WU Xiaoyun2,ZHENG Yuyan1()   

  1. 1. Stomatological center of Shenzhen People′s Hospital, the Second Affiliated Clinical Medical College of Jinan University, Shenzhen 518020, China
    2. Medical records Statistics Office of Shenzhen People′s Hospital, the Second Affiliated Clinical Medical College of Jinan University, Shenzhen 518020, China
  • Received:2019-06-29 Revised:2019-08-28 Online:2019-12-20 Published:2019-12-30
  • Contact: Yuyan ZHENG E-mail:swift_zheng@163.com

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Abstract:

Objective To study the difference in the flora structure and gene function of dental plaque in caries-free and caries-active preschool children by 16S rRNA sequencing. Methods After oral examination of 96 preschool children aged 3-6 years, they were divided into 3 groups according to their decayed, missing and filled surface (dmfs) index: group CF with no caries (n=31), group ECC with moderate early childhood caries (n=29) and group SECC with severe early childhood caries (n=36). Dental plaques were collected, and DNA was extracted and then underwent metagenomic sequencing by Illumina HiSeq 2 500. The bacterial community structure was analyzed by Qiime. Results A total of 12 phyla, 31 classes, 31 orders, 50 families, 92 genera and 1 104 species were found in the dental plaque samples of the three groups, most of which belonged to 9 dominant genera, including Streptococcus, Veillonella, etc. The microbial diversity of the dental plaques in the three groups was similar (P > 0.05). Veillonella, Lactobacillus, Megasphaera, and Scardovia in the SECC group were significantly higher than those in the other two groups. There was a significant correlation between the decayed, missing and filled surface (dmfs) index and the genus. Bifidobacterium, Veillonella, Lactobacillus had a positive correlation with the dmfs index, while Eikenella had a negative correlation with the dmfs index. Indicator species analysis showed that Atopobium, Veillonella, Megasphaera, Scardovia, Lactobacillus and Bifidobacterium had high indicative values in the SECC group. Conclusion There are significant differences in plaque microbiota among preschool children with different caries sensitivities. Veillonella, Megasphaera, Scardovia, Lactobacillus, Bifidobacterium and Atopobium may be the indicator genera of severe early children caries.

Key words: preschool children, caries, caries sensitivity, early childhood caries, severe early childhood caries, dental plaque, microbiota, high-throughput sequencing, Streptococcus, Veillonella

CLC Number: 

  • R788

Figure 1

Principal component analysis"

Figure 2

Composition of 3 groups of dominant genera"

Table 1

Species difference and abundance between CF and ECC"

菌属 丰度平均值 P
CF ECC
拟普雷沃菌属 0.003 0.006 0.046
猫科动物口腔拟杆菌属 8.56 × 10-6 2.49 × 10-4 0.016
微杆菌属 0.006 0 < 0.001

Table 2

Species difference and abundance between CF and SECC"

菌属 丰度平均值 P
CF SECC
韦荣菌属 0.044 0.064 0.029
普雷沃菌7属 0.011 0.019 0.022
乳杆菌属 1.07 × 10-6 4.07 × 10-4 0.001
巨球形菌属 6.10 × 10-5 0.001 0.020
奇异菌属 6.63 × 10-5 4.97 × 10-4 0.002
斯卡多维亚菌属 1.18 × 10-5 2.42 × 10-4 0.001
布雷德菌属 4.28 × 10-6 2.95 × 10-5 < 0.001
艾肯菌属 0.001 4.12 × 10-4 0.046
鞘单胞菌属 1.09 × 10-4 0 0.001
河口微菌属 2.12 × 10-4 6.45 × 10-6 0.017

Table 3

Species difference and abundance between ECC and SECC"

菌属 丰度平均值 P
ECC SECC
乳杆菌属 0 4.07 × 10-4 0.001
巨球形菌属 3.20 × 10-5 0.001 0.011
类斯氏菌属 0 2.58 × 10-5 < 0.001
双歧杆菌属 0 3.50 ×10-5 0.005
奈瑟菌属 0.067 0.044 0.049
拟普雷沃菌属 0.006 0.003 0.032
消化链球菌属 0.001 4.39 × 10-4 0.011
卡托纳菌属 0.001 3.06 × 10-4 0.038
消化球菌属 4.55 × 10-4 1.47 × 10-4 0.001
河口微菌属 1.33 × 10-4 6.45 × 10-6 0.046

Table 4

Indicator species analysis of the three groups"

菌属 指示值 富集组 P
CF ECC SECC
鞘单胞菌属 0.097 0 0 CF 0.043
拟普雷沃菌属 0.255 0.494 0.242 ECC 0.01
消化球菌属 0.158 0.4881 0.118 ECC 0.002
卡托纳菌属 0.247 0.451 0.217 ECC 0.048
消化链球菌属 0.165 0.436 0.105 ECC 0.011
瘤胃菌科TCG.014 0.254 0.406 0.264 ECC 0.038
奇异菌属 0.052 0.141 0.539 SECC 0.007
韦荣菌属 0.269 0.293 0.437 SECC 0.027
巨球形菌属 0.032 0.016 0.479 SECC 0.011
斯卡多维亚菌属 0.004 0.022 0.400 SECC 0.002
乳杆菌属 8.45 × 10-5 0 0.249 SECC 0.004
双歧杆菌属 0 0 0.167 SECC 0.003

Figure 3

ROC curves of classification prediction of CF and ECC, CF and SECC"

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