Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (2): 73-78.DOI: 10.12016/j.issn.2096-1456.2020.02.002

• Basic Study • Previous Articles     Next Articles

Biocompatibility of bone marrow mesenchymal cells on polyetheretherketone and titanium surfaces in vitro

LIAN Keqian1,ZHANG Xin2(),ZHOU Jieyu1,LIAO Yanfen1,SI Shanshan3   

  1. 1. Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
    2. Department of Implantology and Prosthodontics, Stomatological Hospital, Southern Medical University, Guangzhou 511400, China
    3. Department of General Emergency, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China
  • Received:2019-05-23 Revised:2019-11-01 Online:2020-02-20 Published:2020-02-25
  • Contact: Xin ZHANG

骨髓间充质细胞在聚醚醚酮片和纯钛片表面的体外生物相容性比较

连克乾1,张昕2(),周捷宇1,廖燕芬1,司姗姗3   

  1. 1. 中山大学附属第一医院口腔科,广东 广州(510080)
    2. 南方医科大学口腔医院番禺院区种植修复科,广东 广州(511400)
    3. 南方医科大学口腔医院综合急诊科,广东 广州(510280)
  • 通讯作者: 张昕
  • 作者简介:连克乾,副教授,硕士,Email: liankeqian888@163.com
  • 基金资助:
    广东省科技计划项目(2010B031300020)

Abstract:

Objective To compare the in vitro biocompatibility of bone marrow mesenchymal cells on polyetheretherketone (PEEK) and titanium (Ti) surfaces. Methods PEEK and Ti foils with thicknesses of 1 mm and diameters of 10 mm were prepared. First, bone marrow mesenchymal cells were separated and purified by the whole bone marrow adherent culture method in vitro. Then, osteogenesis-induced bone marrow mesenchymal cells were cultivated on the surfaces of the PEEK and Ti foils. Scanning electron microscopy (SEM), the Alamar Blue test, an alkaline phosphatase (ALP) kit and Alizarin Red staining were used to analyze calcium nodules and compare the adhesion, proliferation and osteogenic differentiation ability of bone marrow mesenchymal cells on the surfaces of the PEEK and Ti foils. Results ① The morphology of the bone marrow mesenchymal cells cultured on the PEEK and Ti foils at 1 h, 4 h and 24 h showed no significant differences. ② In the 1 h, 3 h, 1 d and 3 d cultures of the bone marrow mesenchymal cells inoculated on the surfaces of the foils, the number of living cells in the PEEK group was greater than that in the Ti group (P < 0.05). ③ In the 7 d and 14 d osteogenesis-induced cultures of the inoculated bone marrow mesenchymal cells, the ALP activity of the PEEK group cells was significantly greater than that of the Ti group cells (P < 0.05). ④ Semiquantitative analysis after Alizarin Red staining showed that the mineralization degree of the bone marrow mesenchymal cells induced by osteoblasts was greater in the PEEK group than in the Ti group (P < 0.05). Conclusion PEEK has better in vitro biocompatibility than Ti and can better promote cell adhesion, proliferation and osteogenic differentiation compared with Ti, and so it is expected to become a new dental implant material.

Key words: bone marrow mesenchymal cells, polyetheretherketone, titanium, biocompatibility, dental implants, adhesion, proliferation, osteogenesis differentiation, alkaline phosphatase, mineralized nodule

摘要:

目的 比较骨髓间充质细胞在聚醚醚酮(polyetheretherketone, PEEK)和纯钛(titanium, Ti)表面的体外生物相容性。方法 制备厚度为1 mm,直径为10 mm的圆片状PEEK片和Ti片。将PEEK片和Ti片分别与SD乳鼠的骨髓间充质细胞在体外复合并定向成骨诱导培养,采用扫描电镜观察细胞黏附形态、Alamarblue检测细胞黏附与增殖能力、碱性磷酸酶(alkaline phosphatase,ALP)活性及茜素红染色的矿化结节半定量等方法检测成骨分化能力,对比骨髓间充质细胞在PEEK片和Ti片表面的细胞黏附、增殖及成骨分化的能力。结果 ①观察骨髓间充质细胞在PEEK片和Ti片表面培养1 h、4 h、24 h时的细胞形态无明显差异;② 骨髓间充质细胞接种于PEEK片和Ti片表面培养的1 h、3 h、1 d、3 d,PEEK组的活细胞数均高于Ti组(P < 0.05);③在骨髓间充质细胞接种于PEEK片和Ti片表面并成骨诱导矿化培养后的7 d、14 d,PEEK组的细胞ALP活性均高于Ti组的细胞ALP活性(P < 0.05);④成骨诱导的骨髓间充质细胞在PEEK片和Ti片表面接种培养后的7 d、14 d,经茜素红染色后半定量分析,PEEK组的矿化程度高于Ti组(P < 0.05)。结论 PEEK具有优于Ti的体外生物相容性,能更好地促进骨髓间充质细胞的黏附、增殖及成骨分化能力,有望成为牙种植体新材料。

关键词: 骨髓间充质细胞, 聚醚醚酮, 钛, 生物相容性, 牙种植体, 黏附, 增殖, 成骨分化, 碱性磷酸酶, 矿化结节

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