Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (4): 214-218.doi: 10.12016/j.issn.2096-1456.2020.04.002

• Basic Study • Previous Articles     Next Articles

Study on lncRNA ADAMTS9-AS2 promoting invasion and metastasis of salivary adenoid cystic carcinoma

WU Donghui1,ZHU Yunying1,LIANG Jianqiang1,LIN Zhaoyu2,LI Jinsong2()   

  1. 1. Department of Oral Surgery, Stomatology hospital of Haizhu district, Guangzhou 510220, China
    2. Department of Oral & Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510220, China
  • Received:2019-07-17 Revised:2019-11-07 Online:2020-04-20 Published:2020-03-31
  • Contact: Jinsong LI E-mail:lijinsong1967@163.com

Abstract:

Objective To investigate the role of lncRNAs in the invasion and metastasis of salivary adenoid cystic carcinoma (SACC) cells. Methods With SACC-LM as the experimental group and SACC83 as the control group, lncRNA chips were used to screen the differentially expressed lncRNAs. The differentially expressed lncRNAs were further verified by real-time quantitative RT-PCR (qRT-PCR). The invasion and migration abilities of the adenoid cystic carcinoma cell lines before and after transfection with lncRNA siRNAs were detected by invasion and migration experiments. The clinicopathological features and prognosis of patients with different expression of lncRNAs and SACC were analyzed. Results The microarray showed that ADAMTS9-AS2 was highly expressed in the SACC-LM cells. Real-time quantitative RT-PCR further confirmed that ADAMTS9-AS2 was significantly upregulated in the SACC-LM cells. Invasion and migration experiments showed that the invasion and migration were significantly reduced after the expression level of ADAMTS9-AS2 was downregulated (P < 0.001). Analysis of the clinicopathological data showed that ADAMTS9-AS2 was highly expressed in SACC. High expression of ADAMTS9-AS2 was associated with poor prognosis and a high tumor metastasis rate in SACC patients. Conclusion High expression of ADAMTS9-AS2 promotes the migration and invasion of SACC cells. ADAMTS9-AS2 is upregulated in the SACC tissues and is related to a high metastasis rate and poor prognosis.

Key words: long noncoding RNA, lncRNA ADAMTS9-AS2, salivary adenoid cystic carcinoma, adenoid cystic carcinoma cell line with high metastasis, lncRNA expression microarray, invasion, metastasis, RNA interference, epigenetics

CLC Number: 

  • R78

Figure 1

Microarray screening of lncRNA differentially expressed in SACC cell lines and validation ofqRT-PCR a: heat map depicting lncRNAs for which a two fold change in expression was found in the SACC-LM and SACC-83 cells; b: the expression levels of several significantly differentially expressed lncRNAs were validated by qRT-PCR"

Figure 2

The ADAMTS9-AS2 levels were examined in the SACC-LM cells transfected with siRNAs targeting ADAMTS9-AS2 or siRNA controls"

Figure 3

The SACC-LM cells transfected with siR-ADAMTS9-AS2 were subjected to chamber assays"

Figure 4

The ADAMTS9-AS2 expression levels were detected by qRT-PCR in the clinical SACC patients with metastasis or without metastasis and adjacent normal salivary tissues *P<0.05, **P<0.01"

Table 1

Correlation of clinicopathologic status and the expression of lncRNA ADAMTS9-AS2 in the SACC patients"

ADAMTS9-AS2(%)
Clinical Features Overexpression Low
Expression
Chi-Square
Value
P
Gender
Male
Female
24(47.1)
30(58.8)
27(52.9)
21(41.2)
1.417 0.234
Age
<50
≥ 50
22(52.4)
32(53.3)
20(47.6)
28(46.7)
0.009 0.924
Tumor size
T1-2
T3-4
21(41.2)
33(64.7)
30(58.8)
18(35.3)
5.667 0.017
Clinical stages
Ⅰ-Ⅱ
Ⅲ-Ⅳ
19(41.3)
35(62.5)
27(58.7)
21(37.5)
4.554 0.033
Distant Metastasis
No
Yes
17(40.5)
37(61.7)
25(59.5)
23(38.3)
4.453 0.035
Survival status
Survival
Death
15(30.6)
39(73.6)
34(69.4)
14(26.4)
18.872 0.000
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