Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (5): 322-326.doi: 10.12016/j.issn.2096-1456.2020.05.010

• Review Articles • Previous Articles     Next Articles

Research progress on animal models of oral mucositis caused by radiotherapy and chemotherapy

ZOU Xiaolong1,CHEN Yuan1,WANG Yan1,WANG Jiantao2()   

  1. 1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;
    2. State key Laboratory of Biotherapy & Department of Lung Cancer Center and Department of Radiation Oncology, West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2019-02-15 Revised:2019-11-23 Online:2020-05-20 Published:2020-04-24
  • Contact: Jiantao WANG E-mail:wangjiantao@wchscu.cn

RichHTML

11

PDF (PC)

38

Abstract:

Inflammation of the oral mucosa induced by radiotherapy and/or chemotherapy may cause pain, difficulty speaking and swallowing, an increased risk of local and systemic infections, and even interrupt cancer treatment, which can seriously affect a patient′s quality of life. The pathogenesis of oral mucositis is complicated. There is still a lack of prevention and treatment modalities for oral mucositis in the clinic. Animal models play a vital role in exploring the pathogenesis of oral mucositis and developing better prevention and treatment methods. This article reviews the current research progress on the establishment and assessment of animal models of oral mucositis. The literature review results showed that animal models of oral mucositis have been established, such as mouse, rat, and gold hamster models. In the replication of animal models, radiotherapy-induced oral mucositis is generally induced by local single-dose or fractionated irradiation using X-ray equipment, either alone or in combination with the chemotherapy drugs 5-fluorouracil or cisplatin; cesium can also be used used as a radioactive source for local irradiation. Oral mucositis induced by the chemotherapy drug 5-fluorouracil alone is generally mild, so 5-fluorouracil was combined with mechanical trauma or acetic acid. The main methods for assessing oral mucositis are gross observation as well as histopathological observation.

Key words: oral mucositis, radiotherapy, chemotherapy, animal model, assessment, mouse, rat, 5-fluorouracil, cesium

CLC Number: 

  • R781.5
[1] Gruber S, Bozsaky E, Roitinger E , et al. Early inflammatory changes in radiation-induced oral mucositis: effect of pentoxifylline in a mouse model[J]. Strahlenther Onkol, 2017,193(6):499-507.
[2] de Araújo AA, Varela H, de Medeiros CA , et al. Azilsartan reduced TNF-α and IL-1β levels, increased IL-10 levels and upregulated VEGF, FGF, KGF, and TGF-α in an oral mucositis model[J]. PLoS One, 2015,10(2):e0116799.
[3] Gruber S, Hamedinger D, Bozsaky E , et al. Local hypoxia in oral mucosa (mouse) during daily fractionated irradiation - effect of pentoxifylline[J]. Radiother Oncol, 2015,116(3):404-408.
[4] Peterson DE, Boers-Doets CB, Bensadoun RJ , et al. Management of oral and gastrointestinal mucosal injury: ESMO clinical practice guidelines for diagnosis, treatment, and follow-up[J]. Ann Oncol, 2015,26(Suppl 5):v139-v151.
[5] Parkins CS, Fowler JF, Yu S . A murine model of lip epidermal/mucosal reactions to X-irradiation[J]. Radiother Oncol, 1983,1(2):159-165.
[6] Kowaliuk M, Bozsaky E, Gruber S , et al. Systemic administration of heparin ameliorates radiation-induced oral mucositis-preclinical studies in mice[J]. Strahlenther Onkol, 2018,194(7):686-692.
[7] Gruber S, Frings K, Kuess P , et al. Protective effects of systemic dermatan sulfate treatment in a preclinical model of radiation-induced oral mucositis[J]. Strahlenther Onkol, 2018,194(7):675-685.
[8] Frings K, Gruber S, Kuess P , et al. Modulation of radiation-induced oral mucositis by thalidomide: preclinical studies[J]. Strahlenther Onkol, 2016,192(8):561-568.
[9] Gruber S, Schmidt M, Bozsaky E , et al. Modulation of radiation-induced oral mucositis by pentoxifylline: preclinical studies[J]. Strahlenther Onkol, 2015,191(3):242-247.
[10] I T ,Sumita Y, Minamizato T, et al. Bone marrow-derived cell therapy for oral mucosal repair after irradiation[J]. J Dent Res, 2014,93(8):813-820.
[11] Maria OM, Syme A, Eliopoulos N , et al. Single-dose radiation-induced oral mucositis mouse model[J]. Front Oncol, 2016,6:154.
[12] Maria OM, Shalaby M, Syme A , et al. Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis[J]. Cytotherapy, 2016,18(9):1129-1145.
[13] Talwar S, House R, Sundaramurthy S , et al. Inhibition of caspases protects mice from radiation-induced oral mucositis and abolishes the cleavage of RNA-binding protein HuR[J]. J Biol Chem, 2014,289(6):3487-3500.
[14] Mangoni M, Sottili M, Gerini C , et al. A PPAR gamma agonist protects against oral mucositis induced by irradiation in a murine model[J]. Oral Oncol, 2017,64:52-58.
[15] Zhao JS ,Kim KA,de Vera J, et al.R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical Wnt/beta-catenin pathway[J]. Proc Natl Acad Sci U S A, 2009,106(7):2331-2336.
[16] Kim JH, Jung MH, Kim JP , et al. Alpha lipoic acid attenuates radiation-induced oral mucositis in rats[J]. Oncotarget, 2017,8(42):72739-72747.
[17] Nakashima T, Uematsu N, Sakurai K . Intra-oral administration of rebamipide liquid prevents tongue injuries induced by X-ray irradiation in rats[J]. Support Care Cancer, 2017,25(7):2205-2213.
[18] Wu X, Chen P, Sonis ST , et al. A novel Peptide to treat oral mucositis blocks endothelial and epithelial cell apoptosis[J]. Int J Radiat Oncol Biol Phys, 2012,83(3):e409-e415.
[19] Barbosa MM, de Araújo AA, de Araújo Júnior RF , et al. Telmisartan modulates the oral mucositis induced by 5-Fluorouracil in hamsters[J]. Front Physiol, 2018,9:1204.
[20] Harada K, Ferdous T, Kobayashi H , et al. Elemental diet accelerates the recovery from oral mucositis and dermatitis induced by 5-fluorouracil through the induction of fibroblast growth factor 2[J]. Integr Cancer Ther, 2018,17(2):423-430.
[21] Ribeiro SB, de Araújo AA, Araújo Júnior RF , et al. Protective effect of dexamethasone on 5-FU-induced oral mucositis in hamsters[J]. PLoS One, 2017,12(10):e0186511.
[22] Kim DR, Kim J, Oh JY , et al. Protective effect of Salvia miltiorrhiza Bunge on 5-fluorouracil-induced oral mucositis[J]. Int J Mol Med, 2017,40(1):39-46.
[23] Yoshino F, Yoshida A, Nakajima A , et al. Alteration of the redox state with reactive Oxygen species for 5-fluorouracil-induced oral mucositis in hamsters[J]. PLoS One, 2013,8(12):e82834.
[24] Molina Prats P, Gómez Garcia F, Martinez Diaz F , et al. The therapeutic effects of apigenin and dexamethasone on 5-fluorouracil-induced oral mucositis - a pilot study using a Syrian hamster model[J]. J Oral Pathol Med, 2017,46(2):142-147.
[25] Cotomacio CC, Campos L ,Nesadal De Souza D, et al.Dosimetric study of photobiomodulation therapy in 5-FU-induced oral mucositis in hamsters[J]. J Biomed Opt, 2017,22(1):18003.
[26] Koohi-Hosseinabadi O, Andisheh-Tadbir A, Bahadori P , et al. Comparison of the therapeutic effects of the dietary and topical forms of Zizyphus jujuba extract on oral mucositis induced by 5-fluorouracil: a golden hamster model[J]. J Clin Exp Dent, 2015,7(2):e304-e309.
[27] Shimamura Y, Takeuchi I, Terada H , et al. A mouse model for oral mucositis induced by cancer chemotherapy[J]. Anticancer Res, 2018,38(1):307-312.
[28] Chang CT, Hsiang CY, Ho TY , et al. Comprehensive assessment of host responses to 5-fluorouracil-induced oral mucositis through transcriptomic analysis[J]. PLoS One, 2015,10(8):e0135102.
[29] Patel A, Rajesh S, Chandrashekhar VM , et al. A rat model against chemotherapy plus radiation-induced oral mucositis[J]. Saudi Pharm J, 2013,21(4):399-403.
[30] Hitomi S, Ono K, Yamaguchi K , et al. The traditional Japanese medicine hangeshashinto alleviates oral ulcer-induced pain in a rat model[J]. Arch Oral Biol, 2016,66:30-37.
[31] Reda RI, Wen MM, El-Kamel AH . Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis[J]. Int J Nanomedicine, 2017,12:2335-2351.
[32] Bertolini M, Sobue T, Thompson A , et al. Chemotherapy induces oral mucositis in mice without additional noxious stimuli[J]. Transl Oncol, 2017,10(4):612-620.
[33] Sá OMS, Lopes NNF, Alves MTS , et al. Effects of glycine on collagen, PDGF, and EGF expression in model of oral mucositis[J]. Nutrients, 2018,10(10):1485.
[34] Wanzeler AMV, Júnior SMA, Gomes JT , et al. Therapeutic effect of andiroba oil (Carapa guianensis Aubl.) against oral mucositis: an experimental study in golden Syrian hamsters[J]. Clin Oral Investig, 2018,22(5):2069-2079.
[1] LI Mei, CHEN Yifan, CHENG Fanping, HOU Yarong, YIN Wenjuan, ZHAO Jianjiang, HUANG Shaohong. Administrative strategy of dental clinic during the epidemic of Corona Virus Disease 2019 [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 409-415.
[2] ZHANG Cheng,TAO Guiyu,HUANG Li,LV Chunxiao,LI Tiancheng,YIN Xing,ZOU Shujuan. Signal transducer and activator of transcription 3 positively modulates orthodontic tooth movement speed and alveolar bone mass [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 349-354.
[3] WU Fayin,XU Haili. Effect and mechanism of allicin combined with 5-fluorouracil on proliferation and apoptosis of the MEC-1 cell line in mucoepidermoid carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 355-360.
[4] ZHANG Yun,WANG Yanmei,HE Jiacai. Clinical effect of platelet-rich fibrin combined with bone substitute in alveolar ridge preservation [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 361-366.
[5] YANG Yang,YU Zhishuang,DUAN Xiaoyuan,WU Weili,DENG Yi,YAO Ji. Clinical analysis of skeletal malocclusion, axial inclination in patients with short root anomaly of the maxillary central incisors [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 367-371.
[6] XIAO Wenlan,HU Chen,RONG Sheng′an,QU Yili. Clinical application of autogenous dentin as a bone graft material [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 394-398.
[7] REN Lizhi,SUN Rui. New progress in the clinical application of GBR membrane materials [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 404-408.
[8] WANG Qi,LIANG Xinhua. Whole-process quality-based strategies for good clinical practice when using oral medical devices [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 279-284.
[9] WEN Dandan,LV Yalin. Effect of different doses of aspirin on the early osseointegration of titanium alloy implants in rats [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 285-291.
[10] ZHANG Dan,CAO Yubin,LIN Jie. Clinical observation of inferior alveolar nerve block combined with parecoxib sodium intravenous analgesia after mandible orthognaxillary surgery [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 303-306.
[11] ZHANG Sui,HE Dongning. Current status of immediate implant placement in the aesthetic zone of the anterior teeth [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 331-335.
[12] WANG Yamin,ZHOU Zhen,DAO Junfeng,CHEN Qiyue,LIU Wenjing,SONG Guangbao. Evaluation of the effect of concentrated growth factor in guided bone regeneration in maxillary anterior tooth defects [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(4): 236-240.
[13] CAO Cong,ZHOU Nan,ZHANG Kai,JIAN Fan,XU Baohua,MAN Yi. Summary of combined orthodontic and implant treatment in the patients with the congenital absence of maxillary lateral incisors [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(4): 241-245.
[14] DING Feng,SHI Shaojie,SONG Yingliang. Research progress of superhydrophilic implants [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(4): 252-256.
[15] YU Jianmin,MA Jian,QIAO Guangwei,HUANG Yongqing. Progress in evaluating the postoperative effect of cleft lip [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(4): 262-266.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Yuanhong LI, Xinyi FANG, Yu QIU, Lei CHENG. Experimental study on the effects of green tea on salivary flow rate and pH value[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 560 -564 .
[2] Chengzhang LI. Masticatory muscles in occlusion[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(12): 755 -760 .
[3] Mingyu SUN, Hanjiang WU. Research progresses in occult lymph node metastasis of oral squamous cancer[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(1): 61 -65 .
[4] Changtao LI, Jing WU, Li GUAN, Ruiyong WANG. Clinical application of E-MAX glass ceramic onlays in the restorations of the nonvital posterior teeth[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(9): 600 -604 .
[5] Hua ZHANG. The short-term efficacy of implant in the treatment of patients with dentition defect caused by chronic periodontitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(10): 652 -655 .
[6] Xiaodong SHEN, Weiran GUAN, Ronglian JI, Yibing WAN. Epidemiological investigation of dental health status of 6-8 years old children in Zhongkai Hi-Tech Zone of Huizhou[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(10): 664 -667 .
[7] Mengqi LI, Hai LAN. The research progress of anti-caries materials[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(10): 668 -671 .
[8] Tingting ZHU, Minxian MA. Aesthetic evaluation criteria and influencing factors of dental implant[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(10): 672 -676 .
[9] Gang ZHOU, Lingyu LI, Jie CHEN. Clinical report and literature review of right mandibular canine with two roots and two root canals[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(10): 661 -663 .
[10] Xiaowu YAO, Zizheng LU, Shaofei LIU. Analysis of Helicobacter pylori infection in patients with oral lichen planus[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(11): 729 -732 .

Copyright © Editorial Board of Journal of Prevention and Treatment for Stomatological Diseases

This work is licensed under a Creative Commons Attribution 3.0 License.

Address:Guangzhou City Jiangnan Road South 366, Editorial office ofJournal of Prevention and Treatment for Stomatological Diseases, zip code: 510280　Fax:020-34304115　E-mail：kqjbfz@vip.126.com

Supported by:Beijing Magtech

This work is licensed under a Creative Commons Attribution 3.0 License.