Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (6): 355-360.DOI: 10.12016/j.issn.2096-1456.2020.06.003
• Basic Study • Previous Articles Next Articles
Received:
2019-09-05
Revised:
2019-12-12
Online:
2020-06-20
Published:
2020-05-28
Contact:
Fayin WU
通讯作者:
吴发印
作者简介:
徐海丽,医师,硕士,Email: 906760828@qq.com
基金资助:
CLC Number:
WU Fayin,XU Haili. Effect and mechanism of allicin combined with 5-fluorouracil on proliferation and apoptosis of the MEC-1 cell line in mucoepidermoid carcinoma[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 355-360.
吴发印,徐海丽. 大蒜素联合5-氟尿嘧啶对黏液表皮样癌MEC-1细胞增殖与凋亡的作用及机制[J]. 口腔疾病防治, 2020, 28(6): 355-360.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2020.06.003
Groups | 0 mg/L | 25 mg/L | 50 mg/L | 75 mg/L | F | P |
---|---|---|---|---|---|---|
Allicin group | 0 | 4.692 ± 0.795 | 16.889 ± 0.9281) | 28.861 ± 1.3731)2) | 648.490 | 0.001 |
5-FU group | 0 | 5.133 ± 0.422 | 17.011 ± 2.2481) | 27.455 ± 1.4191)2) | 258.275 | 0.001 |
Combined drug group | 0 | 15.874 ± 1.0323)4) | 25.311 ± 1.2471)3)4) | 38.768 ± 4.0931)2)3)4) | 102.483 | 0.001 |
Table 1 Growth inhibition rate of MEC-1 cells 24 hours after treatment x ± s,n=5,%
Groups | 0 mg/L | 25 mg/L | 50 mg/L | 75 mg/L | F | P |
---|---|---|---|---|---|---|
Allicin group | 0 | 4.692 ± 0.795 | 16.889 ± 0.9281) | 28.861 ± 1.3731)2) | 648.490 | 0.001 |
5-FU group | 0 | 5.133 ± 0.422 | 17.011 ± 2.2481) | 27.455 ± 1.4191)2) | 258.275 | 0.001 |
Combined drug group | 0 | 15.874 ± 1.0323)4) | 25.311 ± 1.2471)3)4) | 38.768 ± 4.0931)2)3)4) | 102.483 | 0.001 |
Groups | 0 mg/L | 25 mg/L | 50 mg/L | 75 mg/L | F | P |
---|---|---|---|---|---|---|
Allicin group | 9.387 ± 0.443 | 10.933 ± 0.7941) | 14.040 ± 0.5051)2) | 16.963 ± 1.0091)3) | 107.840 | 0.001 |
5-FU group | 9.387 ± 0.443 | 11.423 ± 0.3161) | 13.120 ± 0.6071)2) | 15.770 ± 1.0291)3) | 84.804 | 0.001 |
Combined drug group | 9.387 ± 0.4434)5) | 13.020 ± 0.2041)4)5) | 17.397 ± 1.4851)2)4)5) | 22.383 ± 1.3351)3)4)5) | 149.104 | 0.001 |
Table 2 Apoptosis rate of MEC-1 cells after 24 h of treatment x ± s,n=5,%
Groups | 0 mg/L | 25 mg/L | 50 mg/L | 75 mg/L | F | P |
---|---|---|---|---|---|---|
Allicin group | 9.387 ± 0.443 | 10.933 ± 0.7941) | 14.040 ± 0.5051)2) | 16.963 ± 1.0091)3) | 107.840 | 0.001 |
5-FU group | 9.387 ± 0.443 | 11.423 ± 0.3161) | 13.120 ± 0.6071)2) | 15.770 ± 1.0291)3) | 84.804 | 0.001 |
Combined drug group | 9.387 ± 0.4434)5) | 13.020 ± 0.2041)4)5) | 17.397 ± 1.4851)2)4)5) | 22.383 ± 1.3351)3)4)5) | 149.104 | 0.001 |
Figure 1 Apoptosis rate of MEC-1 cells after 24 h of treatment different drug groups with 25 mg/L a: control group; b:allicin group, compared with the control group, the apoptosis rate increased; c: 5-FU group, compared with the control group, the apoptosis rate increased; d: combined drug group,compared with other groups, the apoptosis rate increased
Group | Bcl-2 | Bax | Bax/Bcl-2 |
---|---|---|---|
Control group | 0.907 ± 0.015 | 0.353 ± 0.153 | 0.390 ± 0.023 |
Allicin group | 0.580 ± 0.0261) | 1.027 ± 0.0211) | 1.773 ± 0.0891) |
5-FU group | 0.567 ± 0.0311) | 1.040 ± 0.0441) | 1.839 ± 0.1261) |
Combined drug group | 0.343 ± 0.0251)2)3) | 1.430 ± 0.0401)2)3) | 4.182 ± 0.3731)2)3) |
F | 258.289 | 575.773 | - |
P | 0.001 | 0.001 | 0.022 |
Table 3 Ratio of Bax and Bcl-2 protein expression and Bax/Bcl-2 expression in MEC-1 cells x ± s,n=3,%
Group | Bcl-2 | Bax | Bax/Bcl-2 |
---|---|---|---|
Control group | 0.907 ± 0.015 | 0.353 ± 0.153 | 0.390 ± 0.023 |
Allicin group | 0.580 ± 0.0261) | 1.027 ± 0.0211) | 1.773 ± 0.0891) |
5-FU group | 0.567 ± 0.0311) | 1.040 ± 0.0441) | 1.839 ± 0.1261) |
Combined drug group | 0.343 ± 0.0251)2)3) | 1.430 ± 0.0401)2)3) | 4.182 ± 0.3731)2)3) |
F | 258.289 | 575.773 | - |
P | 0.001 | 0.001 | 0.022 |
Figure 2 Expression of Bcl-2 and Bax protein by using allicin and 5-FU alone or in combination 1: allicin group; 2: 5-FU group; 3: combination group; 4: control group
[1] |
Zou X, Liang J, Sun J , et al. Allicin sensitizes hepatocellular cancer cells to anti-tumor activity of 5-fluorouracil through ROS-mediated mitochondrial pathway[J]. J Pharmacol Sci, 2016,131(4):233-240.
DOI URL |
[2] |
Rajasekaran K, Stubbs V, Chen J , et al. Mucoepidermoid carcinoma of the parotid gland a national cancer database study[J]. Am J Otolaryngol, 2018,39(3):321-326.
DOI URL |
[3] | Atarbashi S, Elahi M, Khani M , et al. Immunohistochemical analysis of B-cell lymphoma-2 in pleomorphic adenoma and mucoepidermoid carcinoma[J]. Dent Res J, 2014,11(2):257-263. |
[4] |
Janet-Ofelia GC, Rafael MV, Guillermo GA , et al. Mucoepidermoid carcinoma of the salivary glands: survival and prognostic factors[J]. J Maxillofac Oral Surg, 2017,16(4):431-437.
DOI URL |
[5] | Alfieri S, Granata R, Bergamini C , et al. Systemic therapy in metastatic salivary gland carcinomas: apathology-driven paradigm?[J]. Oral Oncol, 2017,66(3):58-63. |
[6] |
Yousef HN, Aboelwafa HR . The potential protective role of taurine against 5-fluorouracil induced nephrotoxicity in adult male rats[J]. Exp Toxicol Pathol, 2017,69(5):265-274.
DOI URL |
[7] | Jia Y, Du H, Yao M , et al. Chinese Herbal Medicine for myelo suppression induced by chemotherapy or radiotherapy: a systematic review of randomized controlled trials[J]. J Evid Based Complementary Altern Med, 2015,21(1):1-12. |
[8] |
Gruhlke MCH, Antelmann H, Bernhardt J , et al. The human allicin-proteome: S-thioallylation of proteins by the garlic defence substance allicin and its biological effects[J]. Free Radic Biol Med, 2019,131(1):144-153.
DOI URL |
[9] |
Leontiev R, Hohaus N, Jacob C , et al. A comparison of the antibacterial and antifungal activities of thiosulfinate analogues of allicin[J]. Sci Rep, 2018,8(1):6763.
DOI URL |
[10] | Louis XL, Murphy R, Thandapilly SJ , et al. Garlic extracts prevent oxidative stress, hypertrophy and apoptosis in cardiomyocytes: a role for nitric oxide and hydrogen sulfide[J]. BMC Complement Altern Med, 2012,12(1):140. |
[11] | Alex H, Andrea T, Berlin LR , et al. Garlic organosulfur compounds reduce inflammation and oxidative stress during dengue virus infection[J]. Viruses, 2017,9(7):159-169. |
[12] |
Bat-Chen W, Golan T, Peri I , et al. Allicin purified from fresh garlic cloves induces apoptosis in colon cancer cells via Nrf2[J]. Nutr Cancer, 2010,62(7):947-957.
DOI URL |
[13] |
Huang L, Song Y, Lian J , et al. Allicin inhibits the invasion of lung adenocarcinoma cells by altering tissue inhibitor of met alloproteinase/matrix met alloproteinase balance via reducing the activity of phosphoinositide 3-kinase/AKT signaling[J]. Oncol Lett, 2017,14(1):468-474.
DOI URL |
[14] | Li C, Jing H, Ma G , et al. Allicin induces apoptosis through activation of both intrinsic and Ex-trinsic pathways in glioma cells[J]. Mol Med Rep, 2018,17(4):5976-5981. |
[15] |
Chen H, Zhu B, Zhao L , et al. Allicin inhibits proliferation and invasion in vitro and in vivo via SHP-1-mediated STAT3 signaling in cholangiocarcinoma[J]. Cell Physiol Biochem, 2018,47(2):641-653.
DOI URL |
[16] |
Zhang X, Zhu Y, Duan W , et al. Allicininduces apoptosis of the MGC-803 human gastric carcinoma cell line through the p38 mitogen-activated protein kinase/caspase-3 signaling pathway[J]. Mol Med Rep, 2015,11(4):2755-2760.
DOI URL |
[17] | 杨丹, 颜琼, 罗德兰 , 等. 大蒜素对5-氟尿嘧啶治疗肝癌的增效作用及机制[J]. 中国中西医结合杂志, 2019,39(1):72-75. |
Yang D, Yan Q, Luo D , et al. Synergistic effects and mechanisms of allicin on 5-fluorouracil in the treatment of liver cancer[J]. Chin J Chin Wes Med, 2019,39(1):72-75. | |
[18] | 马海滨 . 大蒜素对人喉癌Hep-2细胞增殖、凋亡的影响[J]. 安徽医学. 2017. 21(7):289-292. |
MA HB . Effect of allicin on proliferation and apoptosis of Hep-2 cells in human laryngeal carcinoma[J]. Anhui Med, 2017,21(7):289-292. | |
[19] | 邵佳月, 孙秀威 . 大蒜素联合5-FU对结肠癌HT-29细胞凋亡的影响及机制[J]. 实用肿瘤学杂志, 2018,156(4):7-10. |
SHAO JY, SUN X . Effects and mechanisms of allicin combined with 5-fluorouracil on apoptosis of HT-29 cells in colon cancer[J]. J Appl Oncol, 2018,156(4):7-10. |
[1] | ZHENG Jiawen, ZHAO Qing. The effects of miRNA-155-5p and the circadian clock gene Bmal1 on proliferation and aging in mouse bone marrow mesenchymal stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(9): 630-637. |
[2] | LIU Yingkun, LI Jia, TAO Boqiang, MIN Fenghe, LIU Weiwei. Clinical analysis of 19 cases of maxillary local tissue defects repaired by buccal fat pad flaps [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(9): 658-662. |
[3] | ZHOU Wenkai, WANG Jiaxuan, WANG Yuanfeng, CHEN Meng, TAO Xingru, LIU Zheqi, ZHANG Xu, JI Tong, CAO Wei. Long non-coding RNA DUXAP9 promotes the proliferation and metastasis of head and neck squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(6): 381-389. |
[4] | SU Yutong, HOU Lan, JIANG Bing, ZHENG Genzi, LIU Yuan, WANG Yao. Effects of a red light-emitting diode on the proliferation and osteogenic differentiation of stem cells from the apical papilla [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(5): 321-329. |
[5] | TANG Xiaoyi, ZHANG Pan, WANG Kaiyan, NIU Lina, JIAO Kai, XIAO Yuhong. Research progress on the relationship between mitochondrial function and oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(3): 212-216. |
[6] | GE Wenbin, ZHANG Kun, LUO Shitong, ZHOU Zhi, LIU Yali. Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(2): 89-96. |
[7] | GUO Yan, ZHANG Kai, FAN Xuhui, LI Lifeng. Relationship between serum miR-199a-5p and miR-378a-3p levels and recurrence of infants with proliferative facial hemangioma after propranolol withdrawal [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(2): 97-102. |
[8] | GONG Shengkai, YANG Xiaoshan, DOU Geng, LI Zihan, LIU Siying, WANG Wei, LIU Shiyu. Dental pulp stem cell-derived apoptotic bodies regulate macrophage polarization and inflammatory response [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(1): 12-19. |
[9] | ZHONG qijian,JIN Tingting,PENG Yu,CHEN Weixiong,LI Jinsong. The effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 535-540. |
[10] | WANG Min,JIANG Nan,ZHU Songsong. A novel biomimetic micro/nano hierarchical interface of titanium enhances adhesion, proliferation and osteogenic differentiation of bone marrow mesenchymal cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(4): 226-233. |
[11] | SUN Jingxuan,LI Yanping,PAN Shuang,HE Lina,SUN Xiangyu,ZHANG Shuang,NIU Yumei. Effects of graphene on the proliferation, migration and morphology of dental pulp stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(10): 656-662. |
[12] | CHEN Yongju,HUANG Zixian,CHEN Rui,CHEN Weiliang. Research progress on HPV-positive oropharyngeal carcinoma sensitive to radiation therapy [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(1): 65-68. |
[13] | MA Lingzhi,SHI Jiaozhuang,GE Wenbin,ZHANG Kun,YU Bing,LIU Yali. Effect of miR-21 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(9): 569-574. |
[14] | XU Bin,BI Liangjia. Research progress of sonodynamic therapy in the field of stomatology [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(8): 535-539. |
[15] | CAO Shunshun,WANG Xiaolong,SHU Chuanji,SHAO Jianjie. Inhibitory effect of celecoxib on Cal-27 tongue squamous cell carcinoma cell proliferation [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(7): 427-432. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
This work is licensed under Creative Commons Attribution 3.0 License.