Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (7): 427-432.doi: 10.12016/j.issn.2096-1456.2020.07.004

• Basic Study • Previous Articles     Next Articles

Inhibitory effect of celecoxib on Cal-27 tongue squamous cell carcinoma cell proliferation

CAO Shunshun,WANG Xiaolong(),SHU Chuanji,SHAO Jianjie   

  1. Department of Stomatology, Huangshi Central Hospital of Edong Medical Group, Affiliated Hospital of Hubei Institute of Technology, Huangshi 435002, China
  • Received:2019-03-15 Revised:2020-01-02 Online:2020-07-20 Published:2020-06-04
  • Contact: Xiaolong WANG E-mail:1244755325@qq.com

Abstract:

Objective To explore the inhibitory effect of celecoxib (CELE) on the proliferation of tongue squamous cell carcinoma Cal-27 cells and its mechanism. Methods A CCK-8 assay was used to investigate the cytotoxicity of different concentrations CELE(10, 20, 40, 60, 80, and 100 mol/L) at 24 and 48 h in Cal-27 cells. According to the concentration of CELE, samples were divided into a control group (0 μmol/L) and experimental groups (10, 20, and 40 μmol/L), and cell invasiveness was detected by the Transwell method. The expression levels of c-Myc and Cyclin D1 mRNA were detected with qPCR, and western blots were used to detect the expression of phosphate and tension homologue deleted on chromosome ten (PTEN), phospho-protein kinase B (p-AKT) (Thr308), c-Myc, cyclin D1 and other proteins in Cal-27 cells after 24 h of treatment with different doses of CELE (10, 20, and 40 μ mol/L) and after 6, 12, and 24 h of treatment with 40 μmol/L CELE. Results The different concentrations of CELE were able to inhibit the proliferation of Cal-27 cells, and the higher the concentration of CELE was, the more significant the inhibition of the proliferation of Cal-27 cells was. The cell survival rates of cells exposed to 40 μmol/L CELE were 80% and 75% after 24 and 48 h, respectively. In the four groups of patients, the number of invasive cells was compared, and the results in decreasing order were the control group, 10 μmol/L CELE, 20 μmol/L CELE, and 40 μmol/L CELE. The expression level of c-Myc, cyclin D1 mRNA and the protein in P-AKT (Thr308), c-Myc, and cyclin D1 significantly decreased and the expression of PTEN protein increased in the Cal-27 cells after administration of CELE at different concentrations. Conclusion CELE can inhibit the proliferation of Cal-27 cells, possibly through inhibition of the expression of proliferation signal factors, such as c-Myc and cyclin D1, by activating the PTEN signaling pathway.

Key words: celecoxib, tongue squamous cell carcinoma, tongue squamous cell carcinoma Cal-27, phosphate and tension homology deleted on chromsome ten, protein kinase B, c-Myc, Cyclin D1, proliferation, invasive

CLC Number: 

  • R78

Figure 1

CCK-8 assay to detect the effect of different concentration of CELE on the survival rate of Cal-27 cells CELE: celecoxib"

Figure 2

The effect of different concentrations of CELE on Cal-27 cell invasion (× 200) a: 10 μmol/L CELE group; b: 20 μmol/Lμmol/L CELE group; c: 40 μmol/L CELE group; d: the control group e: #: vs. the control group, P <0.05, ##: vs. the control group, P < 0.01; CELE: celecoxib"

Figure 3

The effect of CELE on c-Myc, Cyclin D1 mRNA expression in Cal-27 cells #: vs. the control group, P < 0.05, ##: vs. the control group, P < 0.01; CELE: celecoxib"

Figure 4

Effects of different concentrations of CELE on the expression of cell proliferation related proteins PTEN, p-Akt (Thr308), c-Myc and cyclin D1 in Cal-27 cells #: vs. the control group, P <0.05, ##: vs. the control group, P < 0.01; CELE: celecoxib; PTEN: phosphate and tension homology deleted on chromsome ten; p-AKT: phospho-protein kinase B"

Figure 5

Effects of 40 μmol/L CELE on the expression of PTEN, p-Akt (Thr308), c-Myc and cyclin D1 in Cal-27 cells at different time #: vs. the control group, P <0.05, ##: vs. the control group, P < 0.01; CELE: celecoxib; PTEN: phosphate and tension homology deleted on chromsome ten; p-AKT: phospho-protein kinase B"

[1] Tota JE, Anderson WF, Coffey C , et al. Rising incidence of oral tongue cancer among white men and women in the United States, 1973-2012[J]. Oral Oncol, 2017,67(1):146-152.
doi: 10.1016/j.oraloncology.2017.02.019
[2] Almangush A, Coletta RD, Bello IO , et al. A simple novel prognostic model for early stage oral tongue cancer[J]. Int J Oral Maxillofac Surg, 2015,44(2):143-150.
doi: 10.1016/j.ijom.2014.10.004
[3] 潘朝斌 . 舌鳞癌的临床综合序列治疗研究进展[J]. 口腔疾病防治, 2018,26(5):273-280.
Pan CB . Progress in the study of comprehensive sequence therapy of tongue squamous cell carcinoma[J]. J Prev Treat Stomatol Dis, 2018,26(5):273-280.
[4] Tian J, Guo F, Chen Y , et al. Nanoliposomal formulation encapsulating celecoxib and genistein inhibiting COX-2 pathway and Glut-1 receptors to prevent prostate cancer cell proliferation[J]. Cancer Lett, 2019,448(5):1-10.
doi: 10.1016/j.canlet.2019.01.002
[5] Tury S, Becette V, Assayag F , et al. Combination of COX-2 expression and PIK3CA mutation as prognostic and predictive markers for celecoxib treatment in breast cancer[J]. Oncotarget, 2016,7(51):85124-85141.
doi: 10.18632/oncotarget.v7i51
[6] Qiu Z, Zhang C, Zhou J , et al. Celecoxib alleviates AKT/c-Met-triggered rapid hepatocarcinogenesis by suppressing a novel COX-2/AKT/FASN cascade[J]. Mol Carcinog, 2019,58(1):31-41.
doi: 10.1002/mc.v58.1
[7] Xu HB, Shen FM, Lv QZ . Celecoxib enhanced the cytotoxic effect of cisplatin in chemo-resistant gastric cancer xenograft mouse models through a cyclooxygenase-2-dependent manner[J]. Eur J Pharmacol, 2016,776(2):1-8.
[8] Gulyas M, Mattsson J, Lindgren A , et al. COX-2 expression and effects of celecoxib in addition to standard chemotherapy in advanced non-small cell lung cancer[J]. Acta Oncol, 2018,57(2):244-250.
[9] Vene R, Tosetti F, Minghelli S , et al. Celecoxib increases EGF signaling in colon tumor associated fibroblasts, modulating EGFR expression and degradation[J]. Oncotarget, 2015,6(14):12310-12325.
doi: 10.18632/oncotarget.v6i14
[10] Ricciardiello L, Ahnen DJ, Lynch PM . Chemoprevention of hereditary colon cancers: time for new strategies[J]. Nat Rev Gastroenterol Hepatol, 2016,13(6):352-361.
doi: 10.1038/nrgastro.2016.56
[11] Mourad M, Jetmore T, Jategaonkar AA , et al. Epidemiological trends of head and neck cancer in the united states: a SEER population study[J]. J Oral Maxillofac Surg, 2017,75(12):2562-2572.
doi: 10.1016/j.joms.2017.05.008
[12] Zeng B, Li Y, Jiang F , et al. LncRNA GAS5 suppresses proliferation, migration, invasion, and epithelial-mesenchymal transition in oral squamous cell carcinoma by regulating the miR-21/PTEN axis[J]. Exp Cell Res, 2019,374(2):365-373.
doi: 10.1016/j.yexcr.2018.12.014
[13] Jia LF, Huang YP, Zheng YF , et al. miR-29b suppresses proliferation, migration, and invasion of tongue squamous cell carcinoma through PTEN-AKT signaling pathway by targeting Sp1[J]. Oral Oncol, 2014,50(11):1062-1071.
doi: 10.1016/j.oraloncology.2014.07.010
[14] He Y, Mingyan E, Wang C , et al. CircVRK1 regulates tumor progression and radioresistance in esophageal squamous cell carcinoma by regulating miR-624-3p/PTEN/PI3K/AKT signaling pathway[J]. Int J Biol Macromol, 2019,125(4):116-123.
doi: 10.1016/j.ijbiomac.2018.11.273
[15] Li L, Pilo GM, Li X , et al. Inactivation of fatty acid synthase impairs hepatocarcinogenesis driven by AKT in mice and humans[J]. J Hepatol, 2016,64(2):333-341.
doi: 10.1016/j.jhep.2015.10.004
[16] Liu R, Li Y, Tian L , et al. Gankyrin drives metabolic reprogramming to promote tumorigenesis, metastasis and drug resistance through activating beta-catenin/c-Myc signaling in human hepatocellular carcinoma[J]. Cancer Lett, 2019,443(2):34-46.
doi: 10.1016/j.canlet.2018.11.030
[17] Ji Y, Wang Z, Li Z , et al. Silencing IGF-II impairs C-myc and N-ras expressions of SMMC-7721 cells via suppressing FAK/PI3K/Akt signaling pathway[J]. Cytokine, 2017,90(1):44-53.
doi: 10.1016/j.cyto.2016.10.008
[18] 钟文德, 刘荣静, 管红兵 , 等. 黄芩苷对人舌鳞癌细胞SCC15的抑制作用[J]. 口腔疾病防治, 2019,27(4):226-230.
Zhong WD, Liu RJ, Guan HB , et al. Inhibitory effect of Baicalin on human tongue squamous cell carcinoma cell line SCC15[J]. J Prev Treat Stomatol Dis, 2019,27(4):226-230.
[19] Liu P, Ge M, Hu J , et al. A functional mammalian target of rapamycin complex 1 signaling is indispensable for c-Myc-driven hepatocarcinogenesis[J]. Hepatology, 2017,66(1):167-181.
doi: 10.1002/hep.v66.1
[20] Ramos-Garcia P, Gonzalez-Moles MA, Ayen A , et al. Asymmetrical proliferative pattern loss linked to cyclin D1 overexpression in adjacent non-tumour epithelium in oral squamous cell carcinoma[J]. Arch Oral Biol, 2019,97(1):12-17.
doi: 10.1016/j.archoralbio.2018.10.007
[1] WU Fayin,XU Haili. Effect and mechanism of allicin combined with 5-fluorouracil on proliferation and apoptosis of the MEC-1 cell line in mucoepidermoid carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(6): 355-360.
[2] ZHANG Sui,HE Dongning. Current status of immediate implant placement in the aesthetic zone of the anterior teeth [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 331-335.
[3] ZENG Fantao,YU Dongsheng. Knockdown of circ_0001273 inhibits the proliferation, migration and invasion of oral squamous cell carcinoma cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 153-157.
[4] SONG Zhifeng,FEI Fei. Clinical study of the minimally invasive extraction of impacted supernumerary teeth in 85 cases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 169-173.
[5] LIAN Keqian,ZHANG Xin,ZHOU Jieyu,LIAO Yanfen,SI Shanshan. Biocompatibility of bone marrow mesenchymal cells on polyetheretherketone and titanium surfaces in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(2): 73-78.
[6] ZHANG Qingbin,GUAN Hongbing. Gradient sequential treatment of temporomandibular joint disorders [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(1): 11-15.
[7] ZHOU Jiaqi,SHU Linjing,XIONG Yi,ZHANG Yixin,XIANG Lin,WU Yingying. Study on the role of FoxO1 in the regulation of osteoblastic metabolism by 1,25(OH)2D3 in a high glucose environment [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(1): 24-29.
[8] HE Jialin, XU Yan, XIE Xianzhe, WANG Tengfei, HUO Dongmei. Effect of platelet-rich fibrin extract on the proliferation of gingival fibroblasts [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(8): 490-495.
[9] Xinyu LIN,Weixiong CHEN,Xinyuan LEI,Zhanpeng OU,song FAN,Jinsong LI. Screening and identification of mitochondrial miRNAs related to chemotherapy resistance in tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 417-422.
[10] Zehong GUO,Yingyuan NING,Shulan XU,Peijun ZHU,Xianglong DING,Yan GAO. Effect of laser-etched pure titanium surface on early proliferation of MG63 cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 435-440.
[11] Fuhua YAN,Lingjun LI. Gingival recession and minimally invasive tunnel technique [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(6): 341-349.
[12] Xinyuan LEI,Xinyu LIN,Zhanpeng OU,Yi RUAN,Jinsong LI. Mitochondrial fission protein 1 determines mitochondrial fission and cisplatin sensitivity in tongue squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(6): 350-354.
[13] XU Shuaimei,ZENG Xiongqun,YUAN Peiyan,LIU Zhongjun,ZENG Shuguang. The role of APOD in the proliferation and migration of human dental pulp cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(6): 355-359.
[14] WANG Anxun. Abnormal glucose and lipid metabolism and oral squamous cell carcinoma [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 137-142.
[15] ZHAN Aiping,ZENG Liwei. Minimally invasive restoration of endodontically treated teeth [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 198-201.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] WU Hongyu,MA Xiaoxin,LU Haixia,FENG Xiping,GU Qin,YE Wei,XIE Yingxin,XIE Danshu,WANG Wenji. Investigation of dental caries and periodontal conditions in maintenance hemodialysis patients[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 313 -317 .
[2] ZHOU Tao,WU Peiyao,YANG Yuqing,CAO Zhiwei,XIE Liang. Research progress on the distribution of primary cilia and related signaling pathways involved in odontogenesis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 318 -321 .
[3] WANG Shipeng,ZHANG Andong,CHENG Li,CHEN Baoyong,WEN Li,LIU Huawei. Basal cell nevus syndrome: 4 case reports and a literature review[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(1): 41 -45 .
[4] ZOU Xiaolong,CHEN Yuan,WANG Yan,WANG Jiantao. Research progress on animal models of oral mucositis caused by radiotherapy and chemotherapy[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 322 -326 .
[5] CUI Ye,HUANG Ziru,WANG Chunlin,LIU Conghua,ZHANG Chao. Effect of three kinds of medium molecular weight proteins on the corrosion resistance of Ni-Ti and stainless steel arch wires[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(2): 83 -89 .
[6] ZHANG Ning,HU Yue,QIAO Chunyan,JI Xin,HAN Ruyu,SUN Lanfang,LI Minghe,HAN Chengmin. Mucoepidermoid carcinoma arising in Warthin’s tumor of the upper lip: a case report and review[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 250 -254 .
[7] LIU Qian,LAN Lufang,YAN Junyi,TIAN Weidong,GUO Shujuan. Research on the surface structure of a dentin matrix with complete demineralization and incomplete demineralization and the osteogenic property promotion of human periodontal ligament cells[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 159 -166 .
[8] LIN Lin,DUAN Ning,WANG Xiang,JIANG Hongliu,WANG Wenmei. Oral verrucous xanthoma in adolescents: a case report and literature review[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(5): 318 -320 .
[9] Shuyu CAI,Xiaoyue LIN,Jin LEI,Song GE. Effects of Porphyromonas gingivalis infection with different fimA genotypes on the secretion of IL-1β, IL-6, and TNF-α by human umbilical vein endothelial cells[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(6): 364 -369 .
[10] LU Weiying,LIU Ping,CHEN Jiawei,LIU Shuying,XU Pingping. Effects of X-ray irradiation on proliferation and RANTES expression of the mouse osteogenic precursor cell line MC3T3-E1[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(10): 621 -626 .
This work is licensed under a Creative Commons Attribution 3.0 License.