Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (7): 458-462.doi: 10.12016/j.issn.2096-1456.2020.07.010

• Review Articles • Previous Articles     Next Articles

Research progress on cell sheet technology and its application in periodontal tissue regeneration

HE Mengjiao1,LI Lisheng2,CHEN Yuling1(),LUO Kai1   

  1. 1. School and Hospital of Stomatology, Fujian Medical University & Key Laboratory of Stomatology, Fujian Province University, Fuzhou 350002, China
    2. Department of Emergency Surgery, Fujian Provincial Hospital, Fuzhou 350001, China
  • Received:2018-12-28 Revised:2020-02-25 Online:2020-07-20 Published:2020-06-04
  • Contact: Yuling CHEN E-mail:chylucky@163.com

Abstract:

At present, conventional periodontal treatment cannot achieve complete and effective periodontal tissue regeneration. Cell sheet technology (CST) is a kind of cell transplantation method without scaffold material that can maintain complete extracellular matrix, important ion channels of cells, growth factor receptors, etc., and ensure the interaction between cells and the extracellular matrix. In this paper, the application and research progress of the cell sheet in the field of periodontal tissue regeneration are reviewed. Different types of seed cells can be prepared into monolayer cell sheet, multilayer cell sheet, cell sheet fragments and cell sheet polymers. Among them, the monalayer cell sheet is easily damaged and requires high deoperator; the multilayer cell sheet shows improved mechanical properties, but its thickness needs to be controlled to avoid cell necrosis. The cell sheet fragment can be used in the narrow space between the alveolar bone and root cementum to reduce the difficulty of operation and improve the mechanical properties of the cell sheet. Cell sheet polymers are three-dimensional structures that can provide strong mechanical support and improve the stability of the cell sheet, but the stability of their biological activity needs to be further improved. In methods for construction of the cell sheet, the antifibrosis and antiangiogenesis properties of the amniotic sheet have shown that this structure is suitable as the matrix of cell culture; the method of using a temperature-sensitive culture dish is simple and easy; continuous induction with vitamin C can retain some important proteins on the cell surface; and the magnetic tissue engineering method can increase cell adhesion and easily form a stable cell sheet. The above methods have their own characteristics. In clinical applications, monolayer cell sheet is mainly used for direct transplantation to the receiving site to construct periodontal tissue; multilayer cell sheet of the same or different species overlap and are then transplanted to the receiving site; and multilayer cell sheet of the same kind are wrapped with scaffold material and then transplanted to the receiving site to construct a three-dimensional structure. Overall, cell sheet technology has shown good potential in periodontal tissue regeneration.

Key words: cell sheet technology, monolayer cell sheet, multilayer cell sheet, cell sheet pellets, periodontal tissue regeneration, alveolar bone regeneration, cementum regeneration, tissue engineering, periodontitis, cell transplantation

CLC Number: 

  • R78
[1] Tassi SA, Sergio NZ, Misawa MYO , et al. Efficacy of stem cells on periodontal regeneration: systematic review of pre-clinical studies[J]. J Periodontal Res, 2017,52(5):793-812.
doi: 10.1111/jre.2017.52.issue-5
[2] Li M, Ma J, Gao Y , et al. Cell sheet technology: a promising strategy in regenerative medicine[J]. Cytotherapy, 2019,21(1):3-16.
doi: 10.1016/j.jcyt.2018.10.013
[3] Duncan TJ, Baba K, Oie Y , et al. A novel method using quantum dots for testing the barrier function of cultured epithelial cell sheets[J]. Invest Ophthalmol Vis Sci, 2015,56(4):2215-2223.
[4] Fujii Y, Kawase-Koga Y, Hojo H , et al. Bone regeneration by human dental pulp stem cells using a helioxanthin derivative and cell-sheet technology[J]. Stem Cell Res Ther, 2018,9(1):24.
doi: 10.1186/s13287-018-0783-7
[5] Na S, Zhang H, Huang F , et al. Regeneration of dental pulp/dentine complex with a three-dimensional and scaffold-free stem-cell sheet-derived pellet[J]. J Tissue Eng Regen Med, 2016,10(3):261-270.
doi: 10.1002/term.v10.3
[6] Akizuki T, Oda S, Komaki M , et al. Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs[J]. J Periodontal Res, 2005,40(3):245-251.
doi: 10.1111/jre.2005.40.issue-3
[7] Hasegawa M, Yamato M, Kikuchi A , et al. Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model[J]. Tissue Eng, 2005,11(3-4):469-478.
doi: 10.1089/ten.2005.11.469
[8] Mu S, Tee BC, Emam H , et al. Culture-expanded mesenchymal stem cell sheets enhance extraction-site alveolar bone growth: an animal study[J]. J Periodontal Res, 2018,53(4):514-524.
doi: 10.1111/jre.2018.53.issue-4
[9] Iwata T, Yamato M, Tsuchioka H , et al. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model[J]. Biomaterials, 2009,30(14):2716-2723.
doi: 10.1016/j.biomaterials.2009.01.032
[10] Ma G, Zhao JL, Mao M , et al. Scaffold-based delivery of bone marrow mesenchymal stem cell sheet fragments enhances new bone formation in vivo[J]. J Oral Maxillofac Surg, 2017,75(1):92-104.
doi: 10.1016/j.joms.2016.08.014
[11] Yang Z, Jin F, Zhang X , et al. Tissue engineering of cementum/periodontal-ligament complex using a novel three-dimensional pellet cultivation system for human periodontal ligament stem cells[J]. Tissue Eng Part C Methods, 2009,15(4):571-581.
doi: 10.1089/ten.tec.2008.0561
[12] 冯国纹, 余丽梅 . 人羊膜应用的研究进展[J]. 生物医学工程学杂志, 2014,31(4):930-934.
Feng GW, Yu LM . Research progress of human amniotic membrane applications[J]. J Biomedical Eng, 2014,31(4):930-934.
[13] Amemiya T, Nakamura T, Yamamoto T , et al. Immunohistochemical study of oral epithelial sheets cultureonamniotic membrane for oral mucosal reconstruction[J]. Biomed Mater Eng, 2010,20(1):37-45.
[14] Takizawa S, Yamamoto T, Honjo K I , et al. Transplantation of dental pulp-derived cell sheets cultured on human amniotic membrane induced to differentiate into bone[J]. Oral Dis, 2019,25(5):1352-1362.
doi: 10.1111/odi.2019.25.issue-5
[15] Okano T, Yamada N, Sakai H , et al. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide)[J]. J Biomed Mater Res, 1993,27(10):1243-1251.
doi: 10.1002/(ISSN)1097-4636
[16] 何梦娇, 江俊, 郑宝玉 , 等. 骨质疏松大鼠骨髓基质细胞膜片的体外构建研究[J]. 中国骨质疏松杂志, 2017,23(8):996-1001.
He MJ, Jiang J, Zheng BY , et al. Construction of osteoporotic rat bone marrow stromal cell sheet in vitro[J]. Chin J Osteoporos, 2017,23(8):996-1001.
[17] Lu Y, Zhang W, Wang J , et al. Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application[J]. Int J Oral Sci, 2019,11(2):1-13.
doi: 10.1038/s41368-018-0031-0
[18] Motoike S, Kajiya M, Komatsu N , et al. Clumps of mesenchymal stem cell/extracellular matrix complexes generated with xeno-free conditions facilitate bone regeneration via direct and indirect osteogenesis[J]. Int J Mol Sci, 2019,20(16):3970.
doi: 10.3390/ijms20163970
[19] Cho H, Tarafder S, Fogge M, Kao K, Lee CH . Periodontal ligament stem/progenitor cells with protein-releasing scaffolds for cementum formation and integration on dentin surface[J]. Connect Tissue Res, 2016,57(6):488-495.
doi: 10.1080/03008207.2016.1191478
[20] Xu Q, Li B, Yuan L , et al. Combination of platelet-rich plasma within periodontal ligament stem cell sheets enhances cell differentiation and matrix production[J]. J Tissue Eng Regen Med, 2017,11(3):627-636.
doi: 10.1002/term.1953
[21] Iwata T, Yamato M, Washio K , et al. Periodontal regeneration with autologous periodontal ligament-derived cell sheets-a safety and efficacy study in ten patients[J]. Regen Ther, 2018,9:38-44.
[22] Herraiz S, Buigues A, Díaz-García C , et al. Fertility rescue and ovarian follicle growth promotion by bone marrow stem cell infusion[J]. Fertil Steril, 2018,109(5):908-918.
[23] Lin J, Shao J, Juan L , et al. Enhancing bone regeneration by combining mesenchymal stem cell sheets with β-TCP/COL-I scaffolds[J]. J Biomed Mater Res B Appl Biomater, 2018,106(5):2037-2045.
doi: 10.1002/jbm.b.34003
[24] Akahane M, Shimizu T, Inagaki Y , et al. Implantation of bone marrow stromal cell sheets derived from old donors supports bone tissue formation[J]. Tissue Eng Regen Med, 2018,15(1):89-100.
doi: 10.1007/s13770-017-0088-4
[25] Li Q, Feng Y, Zhang D , et al. Development of prevascularized cell sheets using single bone marrow mesenchymal stem cell source for tissue regeneration[J]. J Stem Cell Res Ther, 2016,6:351.
[26] Safi IN, Hussein BMA, Al-Shammari AM . In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets[J]. Regen Ther, 2019,11:225-239.
[27] Zhang H, Liu S, Zhu B , et al. Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration[J]. Stem Cell Res Ther, 2016,7(1):168.
doi: 10.1186/s13287-016-0417-x
[28] 李欣, 金作林, 吴琼 , 等. 应用牙周膜干细胞—牙囊干细胞复合细胞膜片同种异体移植修复比格犬牙周组织缺损的研究[J]. 口腔疾病防治, 2016,24(4):204-210.
Li X, Jin ZL, Wu Q , et al. The study of the application of the periodontal membrane stem cells-dental follicle stem cells compoposite membrane allograft in the repairment of Beagle canine periodontal tissue defect[J]. J Prev Treat Stomatol Dis, 2016,24(4):204-210.
[1] QIN Qing,SONG Yang,LIU Jia,LI Qiang. Effects of casein kinase 2 interacting protein-1 on the osteogenic differentiation ability of human periodontal ligament stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(7): 421-426.
[2] WU Hongyu,MA Xiaoxin,LU Haixia,FENG Xiping,GU Qin,YE Wei,XIE Yingxin,XIE Danshu,WANG Wenji. Investigation of dental caries and periodontal conditions in maintenance hemodialysis patients [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 313-317.
[3] ZHANG Limu,LIN Xiaoping. Research progress on the mechanism of C-reactive protein mediated periodontitis and systemic diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 184-188.
[4] LIU Huimin,LI Xiangwei. Research progress of scaffolds for promoting the vascularization of regenerated dental pulp [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(3): 200-204.
[5] XIAO Wenlan,HU Chen,RONG Shengan,ZHU Chenyou,WU Yingying. Application of dentin in bone tissue engineering [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(2): 127-130.
[6] YE Qingsong, HU Fengting, LUO Lihua, Maria Troulis. Research and application of stem cell-derived exosomes in regenerative medicine [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(1): 1-10.
[7] LIU Xiaoyuan,ZHANG Kai,HAN Xiangzhen,ZUO Xinhui,LI Jun,HE Huiyu. Research status of 3D-printed composite PVA bone tissue engineering scaffolds [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(1): 52-55.
[8] LI Jiatong,ZHOU Xuedong,XU Xin,WANG Yan. Research progress of probiotics in the prevention and treatment of oral infectious diseases [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(9): 598-601.
[9] Gang WANG,Peng SUN,Juan LI,Shiguang HUANG. Expression of the receptor of advanced glycation end products in gingival tissue endothelial cells from type 2 diabetic rats with chronic periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(7): 428-434.
[10] DAI Qian,YU Yuanling,HUANG Shiguang. Macrophage IL-33 expression in macrophages of human chronic periapical periodontitis and apical cyst [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(5): 300-303.
[11] HU Huiting,YU Fenglin,ZHAO Yueping. Research progress on the factors influencing the biological characteristics of dental pulp stem cells [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 268-272.
[12] XU Xiongcheng,ZHONG Quan,LUO Kai. Application of 3D printing technology in preparation of scaffolds for periodontal tissue regeneration [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 189-193.
[13] ZHU Yanxia,Gegen tana. Research progress of IL-33/ST2 signaling pathway in bone metabolism [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 202-204.
[14] WANG Ye,LIN Xiaoping. Research progress on the common risk factors and related mechanisms of periodontitis and osteoporosis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 794-798.
[15] WANG Min, GE Song. Research progress on the T cell receptor CDR3 spectral sequence of periodontitis [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(12): 799-803.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] WU Hongyu,MA Xiaoxin,LU Haixia,FENG Xiping,GU Qin,YE Wei,XIE Yingxin,XIE Danshu,WANG Wenji. Investigation of dental caries and periodontal conditions in maintenance hemodialysis patients[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 313 -317 .
[2] ZHOU Tao,WU Peiyao,YANG Yuqing,CAO Zhiwei,XIE Liang. Research progress on the distribution of primary cilia and related signaling pathways involved in odontogenesis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 318 -321 .
[3] WANG Shipeng,ZHANG Andong,CHENG Li,CHEN Baoyong,WEN Li,LIU Huawei. Basal cell nevus syndrome: 4 case reports and a literature review[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(1): 41 -45 .
[4] ZOU Xiaolong,CHEN Yuan,WANG Yan,WANG Jiantao. Research progress on animal models of oral mucositis caused by radiotherapy and chemotherapy[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(5): 322 -326 .
[5] CUI Ye,HUANG Ziru,WANG Chunlin,LIU Conghua,ZHANG Chao. Effect of three kinds of medium molecular weight proteins on the corrosion resistance of Ni-Ti and stainless steel arch wires[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(2): 83 -89 .
[6] ZHANG Ning,HU Yue,QIAO Chunyan,JI Xin,HAN Ruyu,SUN Lanfang,LI Minghe,HAN Chengmin. Mucoepidermoid carcinoma arising in Warthin’s tumor of the upper lip: a case report and review[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 250 -254 .
[7] LIU Qian,LAN Lufang,YAN Junyi,TIAN Weidong,GUO Shujuan. Research on the surface structure of a dentin matrix with complete demineralization and incomplete demineralization and the osteogenic property promotion of human periodontal ligament cells[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(3): 159 -166 .
[8] LIN Lin,DUAN Ning,WANG Xiang,JIANG Hongliu,WANG Wenmei. Oral verrucous xanthoma in adolescents: a case report and literature review[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(5): 318 -320 .
[9] Shuyu CAI,Xiaoyue LIN,Jin LEI,Song GE. Effects of Porphyromonas gingivalis infection with different fimA genotypes on the secretion of IL-1β, IL-6, and TNF-α by human umbilical vein endothelial cells[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(6): 364 -369 .
[10] LU Weiying,LIU Ping,CHEN Jiawei,LIU Shuying,XU Pingping. Effects of X-ray irradiation on proliferation and RANTES expression of the mouse osteogenic precursor cell line MC3T3-E1[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(10): 621 -626 .
This work is licensed under a Creative Commons Attribution 3.0 License.