Journal of Prevention and Treatment for Stomatological Diseases ›› 2020, Vol. 28 ›› Issue (11): 698-704.DOI: 10.12016/j.issn.2096-1456.2020.11.003
• Basic Study • Previous Articles Next Articles
LIU Li(),LAN Lufang,GUO Shujuan(
),WU Yafei
Received:
2019-12-17
Revised:
2020-02-19
Online:
2020-11-20
Published:
2020-09-29
Contact:
Shujuan GUO
通讯作者:
郭淑娟
作者简介:
刘丽,硕士研究生在读,Email: 532544916@qq.com
基金资助:
CLC Number:
LIU Li,LAN Lufang,GUO Shujuan,WU Yafei. A study of susceptibility genes in rare monozygotic twins with generalized aggressive periodontitis[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2020, 28(11): 698-704.
刘丽,蓝露芳,郭淑娟,吴亚菲. 罕见同卵双胞胎侵袭性牙周炎患者易感基因研究[J]. 口腔疾病防治, 2020, 28(11): 698-704.
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Genes | Primer sequences |
---|---|
IL-1β-511 | F:5'-CCAAGAGATAGAGTCTCAGATGG-3' |
R:5'-GGACAAAGTGGAAGACACACAG-3' | |
IL-1β+3953 | F:5'-CTCAGGTGTCTCCAAGAAATCAAA-3' |
R:5'-GCTTTTTTGCTGTGAGTCCCG-3' | |
TNF-α-308 | F:5'-GGCAATGGTTTGAGGGC-3' |
R:5'-GAGGGCGGGAAAGAATCAT-3' | |
FcγR IIIb(NA1) | F:5'-CAGTGGTTTCACAATGTGAA-3' |
R:5'-CATGGACTTCTAGCACCG-3' | |
FcγR IIIb(NA2) | F:5'-CAATGGTACAGCGTGCTT-3' |
R:5'-CTGTACTCTCCACTGTCGTT-3' | |
VDR | F:5'-CAGAGCATGGACAGGGAGCAA-3' |
R:5'-GCAACTCCTCATGGCTGAGGTCTC-3' | |
ER | F:5'-CTGCCACCCTATCTGCTTTTCCTATTCTCC-3' |
R:5'-TCTTTCTCTGCCACCCTGGCGTGGATTATCTGA-3' |
Table 1 The primer sequences
Genes | Primer sequences |
---|---|
IL-1β-511 | F:5'-CCAAGAGATAGAGTCTCAGATGG-3' |
R:5'-GGACAAAGTGGAAGACACACAG-3' | |
IL-1β+3953 | F:5'-CTCAGGTGTCTCCAAGAAATCAAA-3' |
R:5'-GCTTTTTTGCTGTGAGTCCCG-3' | |
TNF-α-308 | F:5'-GGCAATGGTTTGAGGGC-3' |
R:5'-GAGGGCGGGAAAGAATCAT-3' | |
FcγR IIIb(NA1) | F:5'-CAGTGGTTTCACAATGTGAA-3' |
R:5'-CATGGACTTCTAGCACCG-3' | |
FcγR IIIb(NA2) | F:5'-CAATGGTACAGCGTGCTT-3' |
R:5'-CTGTACTCTCCACTGTCGTT-3' | |
VDR | F:5'-CAGAGCATGGACAGGGAGCAA-3' |
R:5'-GCAACTCCTCATGGCTGAGGTCTC-3' | |
ER | F:5'-CTGCCACCCTATCTGCTTTTCCTATTCTCC-3' |
R:5'-TCTTTCTCTGCCACCCTGGCGTGGATTATCTGA-3' |
Figure 1 Clinical data of the monozygotic twins at initial diagnosis a: younger sister's intra-oral photographs; b: younger sister's rotational panoramic radiograph; c: younger sister's diagram of Florida probing; d: elder sister's intra-oral photographs; e: elder sister's rotational panoramic radiograph; f: elder sister's diagram of Florida probing
Groups | PD(mm) | CAL(mm) | BOP | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Elder | Younger | t | P | Elder | Younger | t | P | Elder | Younger | |
Pre-therapy | 3.51 ± 1.17 | 4.65 ± 2.20 | 5.89 | <0.001 | 3.68 ± 1.29 | 6.73 ± 2.86 | 12.57 | <0.001 | 52% | 22% |
Post-treatment | 2.89 ± 0.93 | 3.79 ± 1.69 | 2.94 ± 1.00 | 6.66 ± 2.57 | 15% | 10% | ||||
Value of effect | 0.63 ± 0.92 | 0.86 ± 1.56 | 1.75 | 0.081 | 0.75 ± 0.87 | 0.07 ± 1.45 | 1.97 | 0.049 | ||
t | 5.38 | 4.02 | 5.97 | 0.22 | ||||||
P | <0.001 | <0.001 | <0.001 | 0.826 |
Table 2 Changes in clinical indicators of the monozygotic twins
Groups | PD(mm) | CAL(mm) | BOP | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Elder | Younger | t | P | Elder | Younger | t | P | Elder | Younger | |
Pre-therapy | 3.51 ± 1.17 | 4.65 ± 2.20 | 5.89 | <0.001 | 3.68 ± 1.29 | 6.73 ± 2.86 | 12.57 | <0.001 | 52% | 22% |
Post-treatment | 2.89 ± 0.93 | 3.79 ± 1.69 | 2.94 ± 1.00 | 6.66 ± 2.57 | 15% | 10% | ||||
Value of effect | 0.63 ± 0.92 | 0.86 ± 1.56 | 1.75 | 0.081 | 0.75 ± 0.87 | 0.07 ± 1.45 | 1.97 | 0.049 | ||
t | 5.38 | 4.02 | 5.97 | 0.22 | ||||||
P | <0.001 | <0.001 | <0.001 | 0.826 |
Figure 2 Clinical data of the monozygotic twins after 3 months of basic periodontal treatment a: younger sister's intra-oral photographs; b: younger sister's rotational panoramic radiograph; c: younger sister's diagram of Florida probing; d: elder sister's intra-oral photographs; e: elder sister's rotational panoramic radiograph; f: elder sister's diagram of Florida probing
Groups | PD ≤ 4 mm | 4 mm<PD<6 mm | PD ≥ 6 mm | |
---|---|---|---|---|
Elder | Pre-therapy | 44.6% | 38.1% | 6.5% |
Post-treatment | 14.3% | 13.7% | 0.6% | |
Reduced proportion | 30.3% | 24.4% | 5.9% | |
Younger | Pre-therapy | 64.3% | 25.0% | 39.3% |
Post-treatment | 52.3% | 35.1% | 17.3% | |
Reduced proportion | 12% | -15.1% | 22% |
Table 3 Changes of the ratio about PD in monozygotic twins
Groups | PD ≤ 4 mm | 4 mm<PD<6 mm | PD ≥ 6 mm | |
---|---|---|---|---|
Elder | Pre-therapy | 44.6% | 38.1% | 6.5% |
Post-treatment | 14.3% | 13.7% | 0.6% | |
Reduced proportion | 30.3% | 24.4% | 5.9% | |
Younger | Pre-therapy | 64.3% | 25.0% | 39.3% |
Post-treatment | 52.3% | 35.1% | 17.3% | |
Reduced proportion | 12% | -15.1% | 22% |
Figure 3 The results of PCR-RFLP gel electrophoresis X: younger sister of monozygotic twins; Y: elder sister of monozygotic twins. The results in the X sample are consistent with those in the Y sample
Candidate susceptibility | Genotype | |
---|---|---|
Younger | Elder | |
IL-1β-511 (Ⅰ/Ⅱ) | Ⅰ/Ⅱ | Ⅰ/Ⅱ |
IL-1β+3953 (Ⅰ/Ⅱ) | Ⅰ/Ⅰ | Ⅰ/Ⅰ |
TNF-α-308 (Ⅰ/Ⅱ) | Ⅱ/Ⅱ | Ⅱ/Ⅱ |
FcγR-IIIb (NA1/NA2) | NA1/NA2 | NA1/NA2 |
VDR (A/a, T/t) | A/a, T/t | A/a, T/t |
ER (X/x) | X/x | X/x |
Table 4 The genotype of the six candidate susceptibility genes
Candidate susceptibility | Genotype | |
---|---|---|
Younger | Elder | |
IL-1β-511 (Ⅰ/Ⅱ) | Ⅰ/Ⅱ | Ⅰ/Ⅱ |
IL-1β+3953 (Ⅰ/Ⅱ) | Ⅰ/Ⅰ | Ⅰ/Ⅰ |
TNF-α-308 (Ⅰ/Ⅱ) | Ⅱ/Ⅱ | Ⅱ/Ⅱ |
FcγR-IIIb (NA1/NA2) | NA1/NA2 | NA1/NA2 |
VDR (A/a, T/t) | A/a, T/t | A/a, T/t |
ER (X/x) | X/x | X/x |
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