Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (3): 184-188.DOI: 10.12016/j.issn.2096-1456.2021.03.007

• Review Articles • Previous Articles     Next Articles

Research progress on Streptococcus mutans phages in the prevention of dental caries

LI Yuhan1(),LI Jiaxin1,ZHANG Shiming1,ZHANG Yaohua1,LI Yuqing1,ZENG Jumei2()   

  1. 1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2. West China School of Public Health & West China Fourth Hospital, Sichuan University, Chengdu 610041, China
  • Received:2020-04-30 Revised:2020-06-21 Online:2021-03-20 Published:2021-01-12
  • Contact: Jumei ZENG
  • Supported by:
    National Natural Science Foundation of China(31870065);Fundamental Research Funds for the Central Universities(YJ201985);Undergraduate′s Platform for Innovation and Entrepreneurship Training Program of Sichuan university(201910611603)

变异链球菌噬菌体在龋病防治中的研究进展

李雨含1(),李嘉鑫1,张诗铭1,张耀华1,李雨庆1,曾菊梅2()   

  1. 1.口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院,四川 成都(610041)
    2.四川大学华西公共卫生学院/华西第四医院,四川 成都(610041)
  • 通讯作者: 曾菊梅
  • 作者简介:李雨含,本科在读,Emai:690067735@qq.com
  • 基金资助:
    国家自然科学基金项目(31870065);四川大学中央高校基本科研业务费(YJ201985);四川大学大学生创新创业训练计划(201910611603)

Abstract:

The Streptococcus mutans (S. mutans) phage, as one of the principal pathogenic bacteria of dental caries, is a main cause of the formation and development of dental caries due to its overproliferation in dental plaque biofilms. Bacterial viruses, also known as bacteriophages, have the capability of specifically infecting bacteria and effectively degrading bacterial biofilms. S. mutans phages, therefore, may prevent and control caries. Therapy based on phages has been applied in many fields, but the application of S. mutans phages in caries remains exploratory. This article will review the research progress of S. mutans phages in clinical caries prevention, aiming to provide a new idea for the clinical prevention of caries. The results of the literature review show that the living bacteriophage system has the advantages of high specificity, high affinity and good safety. However, due to its unstable structure, it can be processed into a more stable formulation by freeze-drying, spray drying, adding stability enhancers, or incorporating bacteriophages into ointments, biodegradable polymer matrices or particles to a certain extent to improve stability. The lysozyme produced by phages can digest the bacterial cell wall and release the assembled phage particles, which effectively cleave biofilms. In addition, the antigen binding fragment library for cariogenic pathogens was screened by phage display technology, and the purpose of caries prevention and treatment was achieved by passive immunization of antigen binding fragments. However, the host range of bacteriophages is narrow, so this kind of problem can be overcome by phage combined with traditional therapy or other drug use or cocktail therapy with multiple phages in clinical caries prevention and control.

Key words: Streptococcus mutans, bacteriophage, dental caries, prevention of dental caries, phage therapy, lyase, biofilm, cocktail therapy

摘要:

变异链球菌作为主要致龋病原菌之一,其在牙菌斑生物膜中的过度增殖,是龋病发生发展的重要原因。细菌病毒——噬菌体可特异性感染细菌并有效降解细菌生物膜,因此变异链球菌噬菌体可能用于防治龋病。基于噬菌体的疗法已在多个领域进行临床应用,但变异链球菌噬菌体在龋病中的应用仍处于探索阶段。本文对变异链球菌噬菌体在龋病防治方面的应用进行综述,旨在为龋病的临床预防工作提供新的思路。文献复习结果表明,活噬菌体制剂具有特异性强、亲和力高以及安全性良好的优点,但由于其结构较不稳定,可以通过冻干、喷雾干燥、添加稳定性增强剂等方式,或将噬菌体并入软膏、可生物降解聚合物基质或微粒中加工成较稳定的制剂,在一定程度上提高稳定性;噬菌体产生的裂解酶可以消化细菌细胞壁,从而释放组装好的噬菌体颗粒,其在裂解生物膜中也同样表现优异;通过噬菌体展示技术筛选出针对致龋病原体的抗原结合片段库,利用抗原结合片段的被动免疫也可达到防治龋病的目的。然而,噬菌体的宿主范围狭窄,因此在临床龋病防治工作中可以通过噬菌体联合传统治疗或其他药物使用,或使用多种噬菌体的鸡尾酒治疗。

关键词: 变异链球菌, 噬菌体, 龋病, 龋病防治, 噬菌体疗法, 裂解酶, 生物膜, 鸡尾酒治疗

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