Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (4): 273-278.DOI: 10.12016/j.issn.2096-1456.2021.04.009

• Review Articles • Previous Articles     Next Articles

Effect of mechanical stimulation on the differentiation of stem cells in periodontal bone tissue engineering

LI Tianle(),CHANG Xinnan,QIU Xutong,FU Di,ZHANG Tao()   

  1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-05-22 Revised:2020-10-14 Online:2021-04-20 Published:2021-02-26
  • Contact: Tao ZHANG
  • Supported by:
    National Natural Science Foundation of China(81800947);Sichuan Science and Technology Program(2020YFS0176);Postdoctoral Science Foundation of China(2018M640930)

机械刺激对牙周骨组织工程干细胞分化的影响

李天乐(),常欣楠,仇旭童,付笛,张陶()   

  1. 口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院,四川 成都(610041)
  • 通讯作者: 张陶
  • 作者简介:李天乐,本科生,Email:786864569@qq.com
  • 基金资助:
    国家自然科学基金项目(81800947);四川省科技计划项目(2020YFS0176);中国博士后科学基金项目(2018M640930)

Abstract:

Currently, cell transplantation in combination with scaffold materials are one of the main strategies in periodontal bone tissue engineering. In periodontal bone tissues, the stiffness and spatial structure of tissues such as alveolar bone and cementum differ, and the difference in mechanical properties of scaffolds also has disparate effects on the proliferation and differentiation of stem cells. Accumulating evidence shows that mechanical stimulating factors such as matrix stiffness and scaffold topography modulate biological behaviors of various seeding cells, including adipose-derived stem cells and periodontal ligament stem cells. A hard matrix can promote cytoskeletal stretching of stem cells, leading to nuclear translocation of Yes-associated protein (YAP) and promoting osteogenic differentiation by upregulating alkaline phosphatase (ALP) and osteocalcin (OCN) via the Wnt/β-catenin pathway. The topologic structure of scaffolds can affect cell adhesion and cytoskeletal remodeling, increase the hardness of cells and promote the osteogenic differentiation of stem cells. In this paper, the effects of mechanical stimulation on the differentiation of stem cells in periodontal bone tissue engineering are reviewed.

Key words: periodontal bone tissue engineering, odontogenic stem cells, scaffold material, mechanical stimuli, matrix stiffness, topography, biological behavior, osteogenic differentiation

摘要:

目前,细胞移植及其与支架材料的联合应用仍是牙周骨组织工程的主要策略之一。牙周骨组织中,牙槽骨和牙骨质等组织的硬度及空间结构有明显差异,支架材料的不同机械性能对干细胞的增殖、分化等生物学行为也具有不同诱导作用。研究显示,支架材料的基质硬度和拓扑结构等机械刺激因素参与调控包括脂肪来源间充质干细胞、牙周膜干细胞等在内的种子细胞的多种生物学行为。较硬的基质可促使干细胞的细胞骨架重塑,导致Yes相关蛋白(Yes-associated protein,YAP)核易位而影响基因转录,并通过Wnt/β-catenin通路释放多种关键因子如碱性磷酸酶、骨钙素等,诱导其向成骨向分化。支架材料的拓扑结构也可影响干细胞的粘着力,促进细胞骨架重构并增加细胞硬度而促进其成骨向分化。笔者就机械刺激对牙周骨组织工程干细胞分化的影响作一综述。

关键词: 牙周骨组织工程, 牙源性干细胞, 支架材料, 机械刺激, 基质硬度, 拓扑结构, 生物学行为, 成骨分化

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