Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (6): 383-387.doi: 10.12016/j.issn.2096-1456.2021.06.004

• Basic Study • Previous Articles     Next Articles

Expression of Bex1 and NF-kBp65 in tongue squamous cell carcinoma and its significance

WANG Qianqian1(),DING Dandan1,DING Xiang2,HAN Rui1,HAN Yingying1,ZHOU Meiyun1,XU Jincheng1()   

  1. 1. Department of Stomatology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China
    2. Department of Stomatology, the Second Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China
  • Received:2020-11-04 Revised:2020-12-23 Online:2021-06-20 Published:2021-04-12
  • Contact: Jincheng XU E-mail:1962580034@qq.com;xjch9999@163.com
  • Supported by:
    grants from Key Project of Natural Science Research in Anhui Province(KJ2016A480)

Abstract:

Objective To investigate the expression of brain expressed X-linked gene 1(Bex1) and nuclear factor-kBp65 (NF-kBp65) in tongue squamous cell carcinoma, and its significance. Methods Immunohistochemistry was used to detect the expression of Bex1 and NF-kBp65 in 60 tongue squamous cell carcinoma (TSCC) tissues and adjacent normal tissues, and the relationships between Bex1, NF-kBp65 and the clinicopathological features and prognosis of patients were analyzed. Results The positive expression rate of Bex1 in TSCC was 48.3% (29/60), which was significantly lower than that in adjacent normal tissues 88.3% (53/60) (x2=22.18, P < 0.01). The positive rate of Bex1 was negatively correlated with TNM stage, and the difference was statistically significant (P < 0.05). The positive rate of 63.3% (38/60) in TSCC was significantly higher than 20% (12/60) in adjacent normal tissues (x2=23.18, P < 0.01), the positive rate of NF-kBp65 was positively correlated with TNM stage, and the difference was statistically significant (P < 0.05). According to the Pearson correlation analysis results, the expression of Bex1 and NF-kBp65 in TSCC tissues was negatively correlated (r=-0.302, P=0.019). Kaplan-Meier survival curves showed that the survival rate of Bex1 positive patients was significantly higher than that of Bex1 negative patients. Conclusion In TSCC tissues, the low positive expression rate of Bex1 and the high positive expression rate of NF-kBp65 may promote tumor invasion and metastasis, and the negative expression of Bex1 may be related to the poor prognosis of patients.

Key words: tongue neoplasms, squamous cell carcinoma, invasion, metastasis, TNM stage, Bex1, NF-kBp65, immunohistochemistry, prognosis

CLC Number: 

  • R78

Figure 1

Immunohistochemical examination of Bex1 and NF-kBp65 expression in TSCC and adjacent normal tongue tissues (× 200) a: Bex1 was negatively expressed in cancer tissue; b: Bex1 was positively expressed in adjacent tissues; c: NF-kBp65 was positively expressed in cancer tissue; d: NF-kBp65 was negatively expressed in adjacent tissues; TSCC: tongue squamous cell carcinoma"

Table 1

The expression of Bex1 and NF-kBp65 in TSCC tissues and adjacent normal tissues n(%)"

Groups n Bex1 c2 P NF-kBp65 c2 P
TSCC tissues 60 29(48.3) 22.18 P<0.001 38(63.3) 23.18 < 0.001
Adjacent normal tissues 60 53(88.3) 12(20.0)

Table 2

Relationship between the expression of Bex1 and NF-kBp65 in TSCC and clinicopathologic features of patients with TSCC n(%)"

Clinicopathological variable n Bex1 positive P NF-kBp65 positive P
Gender Male 41 19(46.3) 27(70.7)
Female 19 10(52.6) 0.650 11(47.4) 0.081
Age (years) > 60 32 15(46.9) 17(53.1)
≤ 60 28 14(50.0) 0.809 21(75.0) 0.079
Tumor diameter(cm) < 2 21 16(76.2) 10(47.6)
≥ 2, ≤ 4 25 11(44.0) 18(72.0)
> 4 14 2(14.3 0.001 10(71.4) 0.179
TNM stage Ⅰ+Ⅱ 34 24(70.6) 16(47.1)
Ⅲ+Ⅳ 26 5(19.2) <0.001 22(84.6) 0.003
Histological grade 33 17(51.5) 16(48.5)
15 9(60.0) 12(80.0)
12 3(25.0) 0.168 10(83.3) 0.030
Lymph node metastasis No 41 26(63.4) 19(46.3)
Yes 19 3(15.8) 0.001 19(100) <0.001

Table 3

Correlation of Bex1 and NF-kBp65 expression in TSCC"

Bex1 NF-kBp65 Total r P
+ -
+ 14 15 29
- 24 7 31 -0.302 0.019
Total 38 22 60

Figure 2

Kaplan-Meier survival curves of Bex1-positive and Bex1-negative TSCC patients The blue line represents the survival curve of Bex1 positive patients, and the green line represents the survival curve of Bex1 negative patients; TSCC: tongue squamous cell carcinoma"

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