Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (7): 442-448.DOI: 10.12016/j.issn.2096-1456.2021.07.002

• Basic Study • Previous Articles     Next Articles

Comparison of platelet rich fibrin and acellular dermal matrix in repairing rabbits′ oral mucosal defects

WANG Chengyu1(),FAN Yawei2(),WANG Jue2   

  1. 1. Shanxi Medical University Stomatology Hospital, Taiyuan 030000, China
    2. First Hospital of Shanxi Medical University, Taiyuan 030000, China
  • Received:2020-11-02 Revised:2021-01-25 Online:2021-07-20 Published:2021-04-19
  • Contact: Yawei FAN
  • Supported by:
    Key Research and Development Projects of Shanxi Province(201903D321090)



  1. 1.山西医科大学口腔医学院·口腔医院,山西 太原(030000)
    2.山西医科大学第一医院口腔科,山西 太原(030000)
  • 通讯作者: 范亚伟
  • 作者简介:王承煜,学士,Email:
  • 基金资助:


Objective To investigate the effects of platelet-rich fibrin (PRF) and acellular dermal matrix (ADM) on the repair of oral mucosal defects and to provide the basis for soft tissue growth in oral implant operations.Methods Thirty-six healthy male Japanese big ear rabbits were randomly divided into the PRF group, ADM group, Autograft group (autologous connective tissue transplantation group) and Control group (blank control group); each group contained nine rabbits. Between the midline and the hard palate maxillary incisors, in an 8-mm location preparation and a 10-mm standard mucosa defect, the ADM group, PRF and Autograft group were implanted with ADM, autologous PRF and autologous cornification mucosa, respectively, whereas the control group had wound gauze compression processing at 7, 14, and 21 days to determine the wound healing rate in the area selected by HE staining. The inflammatory grade and average epithelial thickness were observed, and the results were statistically analyzed.Results Compared with the control group, the PRF, ADM and Autograft groups had significantly advanced wound healing (P < 0.05). The wound healing degree in the PRF group was similar to that of the ADM group at all time points (P > 0.05). The wound healing degree in the PRF and ADM groups was lower than that of the Autograft group at each time point (P < 0.05). HE staining results showed that compared with the control group, the levels of inflammation in the PRF group, ADM group and Autograft group were reduced, and the difference was statistically significant (P < 0.05). Nevertheless, there was no significant difference between the PRF, ADM and Autograft groups (P > 0.05). The epithelial thickness in the ADM group was similar to that in the Autograft group (P > 0.05). The epithelial thickness in the ADM group was higher than that in the PRF group at 7 d and 14 d (P < 0.05), but there was no significant difference at 21 d (P > 0.05).Conclusion PRF and ADM have similar healing effects in repairing oral mucosa defects, and they can be used as soft tissue augmentation materials instead of connective tissue transplantation.

Key words: acellular dermal matrix, platelet rich fibrin, autologous connective tissue transplantation, oral mucosa defect, dental implant, wound healing rate, inflammation level, epithelial thickness, animal experiment


目的 探讨富血小板纤维蛋白(platelet rich fibrin,PRF)和脱细胞真皮基质(acellular dermal matrix,ADM)对口腔黏膜缺损修复的效果,为口腔种植手术中实现软组织增量提供实验依据。方法 将36只健康日本雄性大耳兔随机分为PRF组,ADM组,Autograft组(自体结缔组织移植组)及Control组(空白对照组),每组9只,在兔硬腭距上颌门齿8 mm的中间位置制备10 mm标准黏膜缺损。ADM组、PRF组及Autograft组分别植入ADM、自体PRF以及自体黏膜,Control组对创面行纱布按压处理;分别于术后7、14、21 d进行创面愈合率观察,并于术区取材后行HE染色,观察其炎症等级和上皮平均厚度,并对结果进行统计分析。结果 随着时间的增加,PRF组、ADM组、Autograft组分别与Control组相比,可观察到显著促进创面的愈合(P < 0.05);PRF组与ADM组相比在任一时间点创面愈合程度无显著差异(P > 0.05);PRF组、ADM组各时间点创面愈合程度均比Autograft组较低(P < 0.05)。HE染色结果显示,与Control组相比,PRF组、ADM组和Autograft组的炎症等级均有减轻,差异具有统计学意义(P < 0.05);而PRF组、ADM组与Autograft组两两相比,炎症等级无显著差异(P > 0.05)。ADM组与Autograft组相比,上皮厚度无显著差异(P > 0.05);ADM组与PRF组比较,在7 d和14 d时ADM组的上皮厚度更高(P < 0.05),而在21 d时两者无显著性差异(P > 0.05)。结论 PRF与ADM用于口腔黏膜缺损修复,创面愈合效果相似,均可作为代替结缔组织移植的软组织增量材料。

关键词: 脱细胞真皮基质, 富血小板纤维蛋白, 自体结缔组织移植, 口腔黏膜缺损, 牙种植, 创面愈合率, 炎症等级, 上皮厚度, 动物实验

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