Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (7): 462-467.DOI: 10.12016/j.issn.2096-1456.2021.07.005
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WU Ju(),WANG Ling,LIU Xingrong(
)
Received:
2020-10-26
Revised:
2020-12-22
Online:
2021-07-20
Published:
2021-04-19
Contact:
Xingrong LIU
Supported by:
通讯作者:
刘兴容
作者简介:
吴菊,硕士研究生,Email: 基金资助:
CLC Number:
WU Ju,WANG Ling,LIU Xingrong. Inhibitory effect of baicalin on Streptococcus mutans UA159 in vitro [J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(7): 462-467.
吴菊,王玲,刘兴容. 黄芩苷对变异链球菌UA159体外的抑制作用[J]. 口腔疾病防治, 2021, 29(7): 462-467.
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Tube numer | Baicalin concentration (mg/mL) | ΔOD600值 | P |
---|---|---|---|
1 | 24 | 0.046 ± 0.007 | < 0.001 |
2 | 12 | 0.094 ± 0.001 | < 0.001 |
3 | 6 | 0.182 ± 0.004 | < 0.001 |
4 | 3 | 0.265 ± 0.002 | < 0.001 |
5 | 1.5 | 0.377 ± 0.003 | 0.406 |
6 | 0.75 | 0.377 ± 0.005 | 0.544 |
7 | 0.375 | 0.378 ± 0.008 | 0.595 |
8 | 24 | 0.020 ± 0.005 | < 0.001 |
9 | 0 | 0.377 ± 0.007 | 0.496 |
10 | 0 | 0.380 ± 0.005 | - |
Table 1 ΔOD600 value of Streptococcus mutans UA159 at different baicalin concentrations $\bar{x}\pm s$,n=3
Tube numer | Baicalin concentration (mg/mL) | ΔOD600值 | P |
---|---|---|---|
1 | 24 | 0.046 ± 0.007 | < 0.001 |
2 | 12 | 0.094 ± 0.001 | < 0.001 |
3 | 6 | 0.182 ± 0.004 | < 0.001 |
4 | 3 | 0.265 ± 0.002 | < 0.001 |
5 | 1.5 | 0.377 ± 0.003 | 0.406 |
6 | 0.75 | 0.377 ± 0.005 | 0.544 |
7 | 0.375 | 0.378 ± 0.008 | 0.595 |
8 | 24 | 0.020 ± 0.005 | < 0.001 |
9 | 0 | 0.377 ± 0.007 | 0.496 |
10 | 0 | 0.380 ± 0.005 | - |
Time(h) | Baicalin concentration | F | P | |||||||
---|---|---|---|---|---|---|---|---|---|---|
12 mg/mL | 6 mg/mL | 3 mg/mL | 1.5 mg/mL | 0 mg/mL | P1 | P2 | P3 | P4 | ||
4 | 0.123 ± 0.002 | 0.152 ± 0.002 | 0.184 ± 0.004 | 0.192 ± 0.003 | 0.211 ± 0.004 | 349.182 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
8 | 0.174 ± 0.005 | 0.236 ± 0.008 | 0.311 ± 0.005 | 0.375 ± 0.003 | 0.380 ± 0.002 | 895.601 | < 0.001 | < 0.001 | < 0.001 | 0.246 |
12 | 0.211 ± 0.005 | 0.315 ± 0.009 | 0.411 ± 0.003 | 0.458 ± 0.004 | 0.466 ± 0.003 | 1 180.943 | < 0.001 | < 0.001 | < 0.001 | 0.103 |
24 | 0.277 ± 0.003 | 0.327 ± 0.003 | 0.452 ± 0.005 | 0.466 ± 0.003 | 0.473 ± 0.002 | 2 197.682 | < 0.001 | < 0.001 | < 0.001 | 0.068 |
Table 2 ΔOD600 value of Streptococcus mutans UA159 at different concentrations of baicalin at different time points $\bar{x}\pm s$,n=3
Time(h) | Baicalin concentration | F | P | |||||||
---|---|---|---|---|---|---|---|---|---|---|
12 mg/mL | 6 mg/mL | 3 mg/mL | 1.5 mg/mL | 0 mg/mL | P1 | P2 | P3 | P4 | ||
4 | 0.123 ± 0.002 | 0.152 ± 0.002 | 0.184 ± 0.004 | 0.192 ± 0.003 | 0.211 ± 0.004 | 349.182 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
8 | 0.174 ± 0.005 | 0.236 ± 0.008 | 0.311 ± 0.005 | 0.375 ± 0.003 | 0.380 ± 0.002 | 895.601 | < 0.001 | < 0.001 | < 0.001 | 0.246 |
12 | 0.211 ± 0.005 | 0.315 ± 0.009 | 0.411 ± 0.003 | 0.458 ± 0.004 | 0.466 ± 0.003 | 1 180.943 | < 0.001 | < 0.001 | < 0.001 | 0.103 |
24 | 0.277 ± 0.003 | 0.327 ± 0.003 | 0.452 ± 0.005 | 0.466 ± 0.003 | 0.473 ± 0.002 | 2 197.682 | < 0.001 | < 0.001 | < 0.001 | 0.068 |
Baicalin concentration | Adhesion rate(%) | Adhesion inhibition rate(%) |
---|---|---|
12 mg/mL | 28.00 ± 0.36* | 33.83 ± 0.92△ |
6 mg/mL | 31.13 ± 0.47* | 25.77 ± 2.18△ |
3 mg/mL | 33.06 ± 0.20* | 22.18 ± 0.69△ |
1.5 mg/mL | 35.47 ± 0.40* | 16.18 ± 0.71△ |
0 mg/mL | 42.32 ± 0.45 | 0 |
Table 3 Effects of different concentrations of baicalin on adhesion of Streptococcus mutans UA159 $\bar{x}\pm s$,n=3
Baicalin concentration | Adhesion rate(%) | Adhesion inhibition rate(%) |
---|---|---|
12 mg/mL | 28.00 ± 0.36* | 33.83 ± 0.92△ |
6 mg/mL | 31.13 ± 0.47* | 25.77 ± 2.18△ |
3 mg/mL | 33.06 ± 0.20* | 22.18 ± 0.69△ |
1.5 mg/mL | 35.47 ± 0.40* | 16.18 ± 0.71△ |
0 mg/mL | 42.32 ± 0.45 | 0 |
Group | OD600 | P |
---|---|---|
0 h | 0.132 ± 0.003 | < 0.000 |
6 h | 0.247 ± 0.007 | < 0.000 |
12 h | 0.288 ± 0.006 | < 0.000 |
Solvent control | 0.376 ± 0.004 | 0.304 |
Bacteria control | 0.381 ± 0.007 | - |
Table 4 Effects of baicalin on biofilm formation of Streptococcus mutans UA159 $\bar{x}\pm s$,n=3
Group | OD600 | P |
---|---|---|
0 h | 0.132 ± 0.003 | < 0.000 |
6 h | 0.247 ± 0.007 | < 0.000 |
12 h | 0.288 ± 0.006 | < 0.000 |
Solvent control | 0.376 ± 0.004 | 0.304 |
Bacteria control | 0.381 ± 0.007 | - |
Figure 2 Effects of baicalin on the total number of Streptococcus mutans UA159 bacteria was observed under SEM a-b: bacteria control group, UA159 grew well; a: SEM (× 5 000); b: SEM (× 10 000); c-d: added 10 mg/mL baicalin at 0 h, UA159 grew in pairs; c: SEM (× 5 000); d: SEM (× 10 000); e-f: added 10 mg/ml baicalin at 6 h, the total number of UA159 decreased significantly; e: SEM (× 5 000); f: SEM (× 10 000); g-h: added 10 mg/mL baicalin at 12 h, the total number of UA159 decreased significantly; g: SEM (× 5 000); h: SEM (× 10 000); i-j: in the solvent control group, there were no significant differences compared with the bacteria control group; i: SEM (× 5 000); j: SEM (× 10 000); SEM: scanning electron microscope.
[1] |
Mathur VP, Dhillon JK. Dental caries: a disease which needs attention[J]. Indian J Pediatr, 2018,85(3):202-206. doi: 10.1007/s12098-017-2381-6.
DOI URL PMID |
[2] |
Bernabé E, Marcenes W. Can minimal intervention dentistry help in tackling the global burden of untreated dental caries?[J]. Br Dent J, 2020,229(7):487-491. doi: 10.1038/s41415-020-2155-9.
DOI URL PMID |
[3] |
Liao H, Ye J, Gao L, et al. The main bioactive compounds of Scutellaria baicalensis Georgi. for alleviation of inflammatory cytokines: a comprehensive review[J]. Biomed Pharmacother, 2021,133:110917. doi: 10.1016/j.biopha.2020.110917.
URL PMID |
[4] |
Zhang S, Hu B, Xu J, et al. Baicalin suppress growth and virulence-related factors of methicillin-resistant Staphylococcus aureus in vitro and vivo[J]. Microb Pathog, 2020,139:103899. doi: 10.1016/j.micpath.2019.103899.
DOI URL PMID |
[5] |
Zhu X, Mao Y, Guo M, et al. Enhancement of anti-acne effect of Scutellaria baicalensis extract by fermentation with symbiotic fungus Penicillium decumbens[J]. J Biosci Bioeng, 2020,130(5):457-463. doi: 10.1016/j.jbiosc.2020.06.008.
URL PMID |
[6] |
Luo J, Dong BY, Wang K, et al. Baicalin inhibits biofilm formation, attenuates the quorum sensing-controlled virulence and enhances Pseudomonas aeruginosa clearance in a mouse peritoneal implant infection model[J]. PloS one, 2017,12(4):e0176883. doi: 10.1371/journal.pone.0176883.
URL PMID |
[7] | Zhao Q, Chen XY, Martin C. Scutellaria baicalensis, the golden herb from the garden of Chinese medicinal plants[J]. Sci Bull (Beijing), 2016,61(18):1391-1398. doi: 10.1007/s11434-016-1136-5. |
[8] |
Saltos RN, Seoane PR, Aguilera GA, et al. Molecular and serological typing of Streptococcus mutans strains isolated from young Galician population: relationship with the oral health status[J]. Int Microbiol, 2020,23(4):589-596. doi: 10.1007/s10123-020-00132-2.
URL PMID |
[9] | Rincón-Rodríguez RJ, Parada-Sanchez MT, Bedoya-Correa CM, et al. Genetic diversity of Streptococcus mutans serotype c isolated from white spot and cavitated caries lesions from schoolchildren[J]. Arch Oral Biol, 2019,100:33-41. doi: 10.1016/j.archoralbio.2019.02.003. |
[10] | De A, Jorgensen A, Beatty WL, et al. Deficiency of MecA in Streptococcus mutans causes major defects in cell envelope biogenesis, cell division, and biofilm formation[J]. Front Microbiol, 2018,9:2130. doi: 10.3389/fmicb.2018.02130. |
[11] |
Dang MH, Jung JE, Lee DW, et al. Recovery of acid production in Streptococcus mutans biofilms after short-term fluoride treatment[J]. Caries Res, 2016,50(4):363-371. doi: 10.1159/000446408.
URL PMID |
[12] | Tsujii T, Kawada-Matsuo M, Migita H, et al. Antibacterial activity of phellodendron bark against Streptococcus mutans[J]. Microbiol Immunol, 2020,64(6):424-434. doi: 10.1111/1348-0421.12787. |
[13] |
Cui C, Xu H, Yang H, et al. Antibacterial activity of fruiting body extracts from culinary-medicinal winter mushroom, flammulina velutipes (agaricomycetes) against oral pathogen Streptococcus mutans[J]. Int J Med Mushrooms, 2020,22(2):115-124. doi: 10.1615/IntJMedMushrooms.
URL PMID |
[14] |
Elgamily H, Safy R, Makharita R. Influence of medicinal plant extracts on the growth of oral pathogens Streptococcus mutans and lactobacillus acidophilus: an in-vitro study[J]. Open Access Maced J Med Sci, 2019,7(14):2328-2334. doi: 10.3889/oamjms.2019.653.
URL PMID |
[15] | Lemos JA, Palmer SR, Zeng L, et al. The biology of Streptococcus mutans[J]. Microbiol Spectr, 2019,7(1):10. doi: 10.1128/microbiolspec.GPP3-0051-2018. |
[16] | 郭馨蔚, 赵洪岩, 杨瑶瑶, 等. 牙周炎菌群失调研究进展[J]. 口腔疾病防治, 2019,27(11):739-744. doi: 10.12016/j.issn.2096-1456.2019.11.010. |
Guo XW, Zhao HY, Yang YY, et al. Research progress of periodontitis and periodontal dysbiosis[J]. J Prev Treat Stomatol Dis, 2019,27(11):739-744. doi: 10.12016/j.issn.2096-1456.2019.11.010. |
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