Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (12): 828-835.DOI: 10.12016/j.issn.2096-1456.2021.12.005
• Clinical Study • Previous Articles Next Articles
ZHANG Qian1(),CHEN Bin2,YAN Fuhua2(
)
Received:
2021-04-22
Revised:
2021-05-27
Online:
2021-12-20
Published:
2021-08-17
Contact:
Fuhua YAN
Supported by:
通讯作者:
闫福华
作者简介:
张倩,主治医师,博士,Email: 基金资助:
CLC Number:
ZHANG Qian,CHEN Bin,YAN Fuhua. Changes and clinical significance of four biomarkers in gingival crevicular fluid after nonsurgical periodontal therapy[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(12): 828-835.
张倩,陈斌,闫福华. 牙周基础治疗后龈沟液中4种生物标志物水平变化及临床意义[J]. 口腔疾病防治, 2021, 29(12): 828-835.
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Group | IL-6 (pg/site) | IL-10 (pg/site) | TNF-α (pg/site) | ALP (pg/site) |
---|---|---|---|---|
Control | 0.30(0.21,0.61) | 1.34(0.62,1.74) | 1.65(0.62,2.65) | 129.7(22.31,192.8) |
SPP | 2.05(1.16,2.82)1) | 0.62(0.29,0.89)1) | 2.90(2.45,4.16)1) | 272.1(210.3,519.8)1) |
DPP | 6.32(3.50,9.42)1)2) | 0.31(0.10,0.61)1)2) | 6.51(1.45,9.82)1)2) | 905.2(457.9,1744)1)2) |
Table 1 Levels of biomarkers in GCF at periodontitis sites in patients with severe chronic periodontitis at baseline and healthy sites in the control group Median (IQR)
Group | IL-6 (pg/site) | IL-10 (pg/site) | TNF-α (pg/site) | ALP (pg/site) |
---|---|---|---|---|
Control | 0.30(0.21,0.61) | 1.34(0.62,1.74) | 1.65(0.62,2.65) | 129.7(22.31,192.8) |
SPP | 2.05(1.16,2.82)1) | 0.62(0.29,0.89)1) | 2.90(2.45,4.16)1) | 272.1(210.3,519.8)1) |
DPP | 6.32(3.50,9.42)1)2) | 0.31(0.10,0.61)1)2) | 6.51(1.45,9.82)1)2) | 905.2(457.9,1744)1)2) |
Figure 1 Levels of IL-6, TNF-α, IL-10, and ALP in the GCF of deep-pocket and shallow-pocket sites at the baseline and 1, 3, 6 and 12 months after nonsurgical periodontal therapy a: IL-6; b: IL-10; c: TNF-α; d: ALP; *: compared with the baseline, P < 0.005. 1, 3, 6, and 12 months after nonsurgical therapy, the levels of TNF-α and ALP in GCF at the shallow- pocket (PD ≤ 4 mm) and deep-pocket (PD ≥ 6 mm) sites significantly decreased, the level of IL-10 significantly increased (P < 0.005), and the IL-6 level in GCF at the deep-pocket sites significantly decreased. IL-6: interleukin-6; IL-10: interleukin-10; TNF-α: tumor necrosis factor α; ALP: alkaline phosphatase; PD: probing depth
Figure 2 Clinical indicators of deep pocket and shallow pocket sites at the baseline and 1, 3, 6 and 12 months after nonsurgical periodontal therapy a: PD; b: CAL; c: PLI; d: SBI; *: compared with the baseline, P < 0.005. 1, 3, 6, and 12 months after nonsurgical therapy, PD and CAL in the GCF at the shallow-pocket (PD ≤ 4 mm) and deep-pocket (PD ≥ 6 mm) sites significantly decreased, and PLI and SBI in the GCF at the deep pocket sites significantly decreased; PD: probing depth; CAL: clinical attachment loss; PLI: plaque index; SBI: sulcus bleeding index; GCF: gingival crevicular fluid
Parameters | IL-6 | IL-10 | TNF-α | ALP |
---|---|---|---|---|
PD(mm) | P < 0.000 1 | P < 0.000 1 | P < 0.000 1 | P < 0.000 1 |
r=0.758 | r=-0.633 | r=0.697 | r=0.676 | |
CAL (mm) | P < 0.000 1 | P<0.000 1 | P < 0.000 1 | P < 0.000 1 |
r=0.703 | r=-0.594 | r=0.729 | r=0.687 | |
PLI | P=0.003 | P=0.007 | P=0.004 | P=0.003 |
r=0.374 | r=-0.256 | r=0.398 | r=0.372 | |
SBI | P=0.001 | P=0.004 | P=0.003 | P=0.002 |
r=0.477 | r=-0.389 | r=0.380 | r=0.459 |
Table 2 Correlation between periodontal parameters and the levels of biomarkers in GCF
Parameters | IL-6 | IL-10 | TNF-α | ALP |
---|---|---|---|---|
PD(mm) | P < 0.000 1 | P < 0.000 1 | P < 0.000 1 | P < 0.000 1 |
r=0.758 | r=-0.633 | r=0.697 | r=0.676 | |
CAL (mm) | P < 0.000 1 | P<0.000 1 | P < 0.000 1 | P < 0.000 1 |
r=0.703 | r=-0.594 | r=0.729 | r=0.687 | |
PLI | P=0.003 | P=0.007 | P=0.004 | P=0.003 |
r=0.374 | r=-0.256 | r=0.398 | r=0.372 | |
SBI | P=0.001 | P=0.004 | P=0.003 | P=0.002 |
r=0.477 | r=-0.389 | r=0.380 | r=0.459 |
Biomarkers and clinical parameters | 1 month/baseline | 3 months/1 month | 6 months/3 months | 12 months/6 months |
---|---|---|---|---|
sites/total, ratio | 8/120, 6.67% | 12/120, 10% | 10/120, 8.33% | 12/120, 10% |
IL-6(pg) | 6.72±1.071)/5.71±0.87 | 5.16±0.481)/4.18±0.45 | 6.31±0.891)/4.71±0.50 | 6.10±1.001)/4.46±0.74 |
TNF-α(pg) | 6.99±0.931)/5.67±0.62 | 4.72±0.611)/3.85±0.70 | 5.70±0.841)/4.08±0.49 | 5.54±0.781)/4.33±0.86 |
IL-10(pg) | 0.37±0.041)/0.50±0.03 | 0.42±0.061)/0.60±0.04 | 0.47±0.071)/0.77±0.10 | 0.80±0.171)/1.30±0.17 |
ALP(pg) | 1 372.45±221.141)/1 071.29±208.25 | 1 091.54±243.481)/514.36±35.28 | 672.39±103.691)/441.23±70.15 | 642.34±130.291)/343.27±52.36 |
CAL(mm) | 7.44±0.821)/5.31±0.75 | 7.12±0.611)/4.96±0.54 | 7.05±0.721)/4.75±0.75 | 7.33±0.721)/5.08±0.70 |
Table 3 Levels of biomarkers in GCF at the sites of periodontitis progression 1, 3, 6, and 12 months after nonsurgical periodontal therapy $\bar{x}±s$
Biomarkers and clinical parameters | 1 month/baseline | 3 months/1 month | 6 months/3 months | 12 months/6 months |
---|---|---|---|---|
sites/total, ratio | 8/120, 6.67% | 12/120, 10% | 10/120, 8.33% | 12/120, 10% |
IL-6(pg) | 6.72±1.071)/5.71±0.87 | 5.16±0.481)/4.18±0.45 | 6.31±0.891)/4.71±0.50 | 6.10±1.001)/4.46±0.74 |
TNF-α(pg) | 6.99±0.931)/5.67±0.62 | 4.72±0.611)/3.85±0.70 | 5.70±0.841)/4.08±0.49 | 5.54±0.781)/4.33±0.86 |
IL-10(pg) | 0.37±0.041)/0.50±0.03 | 0.42±0.061)/0.60±0.04 | 0.47±0.071)/0.77±0.10 | 0.80±0.171)/1.30±0.17 |
ALP(pg) | 1 372.45±221.141)/1 071.29±208.25 | 1 091.54±243.481)/514.36±35.28 | 672.39±103.691)/441.23±70.15 | 642.34±130.291)/343.27±52.36 |
CAL(mm) | 7.44±0.821)/5.31±0.75 | 7.12±0.611)/4.96±0.54 | 7.05±0.721)/4.75±0.75 | 7.33±0.721)/5.08±0.70 |
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