Journal of Prevention and Treatment for Stomatological Diseases ›› 2022, Vol. 30 ›› Issue (2): 97-102.DOI: 10.12016/j.issn.2096-1456.2022.02.004

• Basic Study • Previous Articles     Next Articles

Relationship between serum miR-199a-5p and miR-378a-3p levels and recurrence of infants with proliferative facial hemangioma after propranolol withdrawal

GUO Yan1(), ZHANG Kai2, FAN Xuhui3, LI Lifeng4()   

  1. 1. Department of Dental Emergency, Xi′an Jiaotong University Hospital of Stomatology, Xi′an 710001, China
    2. Department of Plastic Surgery, Hospital of Stomatology, Air Force Military Medical University, Xi′an 710032, China
    3. Department of Oral and Maxillofacial Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China
    4. Department of Traumatology and Plastic Surgery, Hospital of Stomatology, Xi′an Jiaotong University, Xi′an 710001, China
  • Received:2021-07-09 Revised:2021-08-25 Online:2022-02-20 Published:2021-11-25
  • Contact: LI Lifeng
  • Supported by:
    Shaanxi Province Social Development Science and Technology Project(2017SF10325)

血清miR-199a-5p、miR-378a-3p水平与婴幼儿增殖期面部血管瘤普萘洛尔停药后复发的关系

郭彦1(), 张凯2, 范戌辉3, 李立峰4()   

  1. 1.西安交通大学口腔医院口腔急诊科,陕西 西安(710001)
    2.空军军医大学口腔医院整形外科,陕西 西安(710032)
    3.河北医科大学第二医院口腔颌面外科,河北 石家庄(050000)
    4.西安交通大学口腔医院创伤整形外科,陕西 西安(710001)
  • 通讯作者: 李立峰
  • 作者简介:郭彦,医师,硕士,Email: gra567@126.com
  • 基金资助:
    陕西省社会发展科技攻关项目(2017SF10325)

Abstract:

Objective To investigate the relationship between serum miR-199a-5p and miR-378a-3p levels and the recurrence of infants with proliferative facial hemangioma relapsed after propranolol withdrawal in infants. Methods Ninety-three infants with proliferative facial hemangioma were selected, all of whom received propranolol treatment. The recurrence of the hemangioma after treatment was followed up, and the children were divided into a recurrence group (23 patients) and a nonrecurrence group (70 patients). Venous blood was collected before and after treatment, and the serum levels of miR-199a-5p and miR-378a-3p were detected by qRT-PCR, and the relationship between the serum levels of miR-199a-5p and miR-378a-3p and the recurrence of proliferative facial hemangioma relapsed after propranolol withdrawal in infants was analyzed. Results The serum expressions levels of miR-199a-5p and miR-378a-3p in non-relapsed group were increased after treatment compared with before treatment (P < 0.05), and there was no significant difference in the levels of miR-199a-5p and miR-378a-3p in the serum of the recurrence group after treatment compared with before treatment (P > 0.05). After treatment, the levels of miR-199a-5p and miR-378a-3p in the serum of the recurrence group were lower than those of the nonrecurrence group (P < 0.05). After treatment, the serum levels of miR-199a-5p and miR-378a-3p in patients with Ⅲ ~Ⅳ Norm grade hemangioma were higher than those in patients with Ⅰ~Ⅱ Norm grade hemangioma (P < 0.05). Logistic regression analysis showed that the low expression of miR-199a-5p, low expression of miR-378a-3p and tumor grade Ⅰ~Ⅱ after treatment were risk factors for the recurrence of proliferative facial hemangioma after propranolol withdrawal in infants (P < 0.05). Conclusion Low serum levels of miR-199a-5p and miR-378a-3p are associated with the recurrence of proliferative facial hemangioma after propranolol withdrawal in infants.

Key words: micro ribonucleic acid, miR-199a-5p, miR-378a-3p, propranolol, infant, hemangioma, recurrence, endothelial cell proliferation

摘要:

目的 探讨血清miR-199a-5p、miR-378a-3p水平与婴幼儿增殖期面部血管瘤普萘洛尔停药后复发的关系。方法 增殖期面部血管瘤患儿93例,均接受普萘洛尔治疗,随访治疗后血管瘤复发情况,并将患儿分为复发组(23例)和无复发组(70例)。治疗前、后采集静脉血,qRT-PCR检测血清miR-199a-5p、miR-378a-3p表达,分析血清miR-199a-5p、miR-378a-3p表达与增殖期面部血管瘤婴幼儿普萘洛尔停药后复发的关系。结果 无复发组治疗后血清miR-199a-5p、miR-378a-3p表达较治疗前增高(P<0.05),复发组治疗后血清miR-199a-5p、miR-378a-3p表达与治疗前比较差异无统计学意义(P>0.05)。复发组治疗后血清miR-199a-5p、miR-378a-3p表达低于无复发组(P<0.05)。治疗后瘤体分级Ⅲ~Ⅳ级患儿血清miR-199a-5p、miR-378a-3p表达高于Ⅰ~Ⅱ级患儿(P<0.05)。Logistic回归分析结果显示miR-199a-5p低表达、miR-378a-3p低表达、治疗后瘤体分级Ⅰ~Ⅱ级是增殖期面部血管瘤婴幼儿普萘洛尔停药后复发的危险因素(P<0.05)。结论 血清miR-199a-5p、miR-378a-3p低表达与增殖期面部血管瘤婴幼儿普萘洛尔停药后血管瘤复发有关。

关键词: 微小RNA, miR-199a-5p, miR-378a-3p, 普萘洛尔, 婴幼儿, 血管瘤, 复发, 内皮细胞增殖

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